Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 65.04
WM115-P10
E10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.89 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 62.75
WM115-P10
E11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 865.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.83
WM115-P10
E2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 783.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 112.07
WM115-P10
E3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 564.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 119.19
WM115-P10
E4
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 138.23
WM115-P10
E5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 854.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.16 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 177.75
WM115-P10
E6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 855.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.12 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 131.33
WM115-P10
E7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 773.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 66.64
WM115-P10
E8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 627.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 181.70 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 55.58
WM115-P10
E9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 174.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 187.84
WM115-P10
F10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 54.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 162.53
WM115-P10
F11
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 461.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 179.92
WM115-P10
F2
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 495.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 166.97
WM115-P10
F3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 427.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 166.64
WM115-P10
F4
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 444.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.41
WM115-P10
F5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.56 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.56
WM115-P10
F6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 781.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 175.40
WM115-P10
F7
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 56.65
WM115-P10
F8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 42.90
WM115-P10
F9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 52.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 137.72
WM115-P10
G10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 67.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 50.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.52
WM115-P10
G11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.25
WM115-P10
G2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 146.15
WM115-P10
G3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 161.81
WM115-P10
G4