Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 355.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.08 NGFR rabbit IgG(H+L) / donkey NGFR 1689.83
WM115-P7
F10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.19 NGFR rabbit IgG(H+L) / donkey NGFR 1560.44
WM115-P7
F11
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.39 NGFR rabbit IgG(H+L) / donkey NGFR 1612.47
WM115-P7
F2
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 633.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.00 NGFR rabbit IgG(H+L) / donkey NGFR 1445.64
WM115-P7
F3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.14 NGFR rabbit IgG(H+L) / donkey NGFR 1178.82
WM115-P7
F4
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 606.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.68 NGFR rabbit IgG(H+L) / donkey NGFR 959.04
WM115-P7
F5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 951.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.75 NGFR rabbit IgG(H+L) / donkey NGFR 1296.97
WM115-P7
F6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 879.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.65 NGFR rabbit IgG(H+L) / donkey NGFR 1217.65
WM115-P7
F7
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 456.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.25 NGFR rabbit IgG(H+L) / donkey NGFR 1262.01
WM115-P7
F8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.45 NGFR rabbit IgG(H+L) / donkey NGFR 1423.79
WM115-P7
F9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 262.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 104.56 NGFR rabbit IgG(H+L) / donkey NGFR 2218.58
WM115-P7
G10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.22 NGFR rabbit IgG(H+L) / donkey NGFR 1893.18
WM115-P7
G11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.82 NGFR rabbit IgG(H+L) / donkey NGFR 2334.75
WM115-P7
G2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 608.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1797.50
WM115-P7
G3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.20 NGFR rabbit IgG(H+L) / donkey NGFR 1201.41
WM115-P7
G4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 594.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 982.67
WM115-P7
G5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 822.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.48 NGFR rabbit IgG(H+L) / donkey NGFR 1657.73
WM115-P7
G6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 938.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.08 NGFR rabbit IgG(H+L) / donkey NGFR 1268.21
WM115-P7
G7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 1552.62
WM115-P7
G8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.65 NGFR rabbit IgG(H+L) / donkey NGFR 1548.22
WM115-P7
G9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2909.88
WM115-P8
B10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2839.07
WM115-P8
B11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 901.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3152.18
WM115-P8
B2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 721.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3230.38
WM115-P8
B3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1118.36
WM115-P8
B4