Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.52 NGFR rabbit IgG(H+L) / donkey NGFR 1131.58
WM115-P7
C5
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 862.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.60 NGFR rabbit IgG(H+L) / donkey NGFR 1551.63
WM115-P7
C6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 927.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.21 NGFR rabbit IgG(H+L) / donkey NGFR 1352.24
WM115-P7
C7
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.52 NGFR rabbit IgG(H+L) / donkey NGFR 1444.39
WM115-P7
C8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 420.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.56 NGFR rabbit IgG(H+L) / donkey NGFR 1308.11
WM115-P7
C9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.16 NGFR rabbit IgG(H+L) / donkey NGFR 2395.84
WM115-P7
D10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.86 NGFR rabbit IgG(H+L) / donkey NGFR 2118.67
WM115-P7
D11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 632.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 2351.87
WM115-P7
D2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 562.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.92 NGFR rabbit IgG(H+L) / donkey NGFR 2073.06
WM115-P7
D3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.52 NGFR rabbit IgG(H+L) / donkey NGFR 1693.04
WM115-P7
D4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 557.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.39 NGFR rabbit IgG(H+L) / donkey NGFR 1122.76
WM115-P7
D5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 639.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.26 NGFR rabbit IgG(H+L) / donkey NGFR 2031.39
WM115-P7
D6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 824.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.30 NGFR rabbit IgG(H+L) / donkey NGFR 1644.62
WM115-P7
D7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.16 NGFR rabbit IgG(H+L) / donkey NGFR 1724.50
WM115-P7
D8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.93 NGFR rabbit IgG(H+L) / donkey NGFR 1722.13
WM115-P7
D9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 339.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 NGFR rabbit IgG(H+L) / donkey NGFR 1247.51
WM115-P7
E10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.06 NGFR rabbit IgG(H+L) / donkey NGFR 1300.91
WM115-P7
E11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 949.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 212.74 NGFR rabbit IgG(H+L) / donkey NGFR 916.12
WM115-P7
E2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 875.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.54 NGFR rabbit IgG(H+L) / donkey NGFR 783.21
WM115-P7
E3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.51 NGFR rabbit IgG(H+L) / donkey NGFR 1122.90
WM115-P7
E4
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.65 NGFR rabbit IgG(H+L) / donkey NGFR 868.81
WM115-P7
E5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 957.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.32 NGFR rabbit IgG(H+L) / donkey NGFR 1193.29
WM115-P7
E6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 996.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.28 NGFR rabbit IgG(H+L) / donkey NGFR 1205.16
WM115-P7
E7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 914.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.27 NGFR rabbit IgG(H+L) / donkey NGFR 1096.13
WM115-P7
E8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 849.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.62 NGFR rabbit IgG(H+L) / donkey NGFR 923.15
WM115-P7
E9