Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 381.76
Colo858-P4
G5
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 489.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.38
Colo858-P4
G6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.34
Colo858-P4
G7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.05
Colo858-P4
G8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 257.41
Colo858-P4
G9
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1923.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 239.88 NGFR rabbit IgG(H+L) / donkey NGFR 87.10
Colo858-P7
B10
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1425.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 200.45 NGFR rabbit IgG(H+L) / donkey NGFR 73.01
Colo858-P7
B11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3260.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 400.81 NGFR rabbit IgG(H+L) / donkey NGFR 142.45
Colo858-P7
B2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3359.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.82 NGFR rabbit IgG(H+L) / donkey NGFR 133.84
Colo858-P7
B3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2805.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 343.41 NGFR rabbit IgG(H+L) / donkey NGFR 118.72
Colo858-P7
B4
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3068.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.57 NGFR rabbit IgG(H+L) / donkey NGFR 101.45
Colo858-P7
B5
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2264.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.71 NGFR rabbit IgG(H+L) / donkey NGFR 101.71
Colo858-P7
B6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2022.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.98 NGFR rabbit IgG(H+L) / donkey NGFR 131.68
Colo858-P7
B7
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3107.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 379.72 NGFR rabbit IgG(H+L) / donkey NGFR 160.75
Colo858-P7
B8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3266.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 411.78 NGFR rabbit IgG(H+L) / donkey NGFR 137.05
Colo858-P7
B9
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 838.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.35 NGFR rabbit IgG(H+L) / donkey NGFR 802.84
Colo858-P7
C10
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 752.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.15 NGFR rabbit IgG(H+L) / donkey NGFR 792.07
Colo858-P7
C11
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1575.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 255.59 NGFR rabbit IgG(H+L) / donkey NGFR 300.64
Colo858-P7
C2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1344.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.89 NGFR rabbit IgG(H+L) / donkey NGFR 147.29
Colo858-P7
C3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1375.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.63 NGFR rabbit IgG(H+L) / donkey NGFR 642.79
Colo858-P7
C4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1215.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.44 NGFR rabbit IgG(H+L) / donkey NGFR 547.76
Colo858-P7
C5
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 918.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.74 NGFR rabbit IgG(H+L) / donkey NGFR 383.73
Colo858-P7
C6
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1044.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.15 NGFR rabbit IgG(H+L) / donkey NGFR 177.98
Colo858-P7
C7
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1450.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.96 NGFR rabbit IgG(H+L) / donkey NGFR 177.02
Colo858-P7
C8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1101.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.56 NGFR rabbit IgG(H+L) / donkey NGFR 152.71
Colo858-P7
C9