Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 150.91 | p-Rb(S807/S811) | 54.62 | p-S6(S235/S236 | 93.11 | 2 | Colo858-signal2 | C5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 174.16 | p-Rb(S807/S811) | 73.24 | p-S6(S235/S236 | 166.46 | 2 | Colo858-signal2 | C6 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 274.60 | p-Rb(S807/S811) | 140.30 | p-S6(S235/S236 | 340.20 | 2 | Colo858-signal2 | C7 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 349.40 | p-Rb(S807/S811) | 166.03 | p-S6(S235/S236 | 385.58 | 2 | Colo858-signal2 | C8 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 301.75 | p-Rb(S807/S811) | 164.37 | p-S6(S235/S236 | 394.95 | 2 | Colo858-signal2 | C9 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 280.73 | p-Rb(S807/S811) | 169.28 | p-S6(S235/S236 | 409.65 | 1 | Colo858-signal2 | D10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 323.83 | p-Rb(S807/S811) | 165.88 | p-S6(S235/S236 | 385.36 | 1 | Colo858-signal2 | D11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 315.08 | p-Rb(S807/S811) | 253.54 | p-S6(S235/S236 | 620.64 | 1 | Colo858-signal2 | D2 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 355.48 | p-Rb(S807/S811) | 234.13 | p-S6(S235/S236 | 567.56 | 1 | Colo858-signal2 | D3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 332.96 | p-Rb(S807/S811) | 207.97 | p-S6(S235/S236 | 501.48 | 1 | Colo858-signal2 | D4 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 367.65 | p-Rb(S807/S811) | 192.54 | p-S6(S235/S236 | 440.93 | 1 | Colo858-signal2 | D5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 319.80 | p-Rb(S807/S811) | 180.35 | p-S6(S235/S236 | 422.77 | 1 | Colo858-signal2 | D6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 302.14 | p-Rb(S807/S811) | 172.24 | p-S6(S235/S236 | 407.88 | 1 | Colo858-signal2 | D7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 288.25 | p-Rb(S807/S811) | 170.31 | p-S6(S235/S236 | 401.43 | 1 | Colo858-signal2 | D8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 293.86 | p-Rb(S807/S811) | 167.77 | p-S6(S235/S236 | 396.05 | 1 | Colo858-signal2 | D9 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 321.22 | p-Rb(S807/S811) | 160.77 | p-S6(S235/S236 | 385.74 | 2 | Colo858-signal2 | E10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 357.02 | p-Rb(S807/S811) | 174.95 | p-S6(S235/S236 | 420.53 | 2 | Colo858-signal2 | E11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 370.03 | p-Rb(S807/S811) | 260.57 | p-S6(S235/S236 | 614.60 | 2 | Colo858-signal2 | E2 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 340.93 | p-Rb(S807/S811) | 241.66 | p-S6(S235/S236 | 583.63 | 2 | Colo858-signal2 | E3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 343.78 | p-Rb(S807/S811) | 196.27 | p-S6(S235/S236 | 463.89 | 2 | Colo858-signal2 | E4 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 302.44 | p-Rb(S807/S811) | 181.09 | p-S6(S235/S236 | 433.45 | 2 | Colo858-signal2 | E5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 370.36 | p-Rb(S807/S811) | 181.15 | p-S6(S235/S236 | 424.43 | 2 | Colo858-signal2 | E6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 333.07 | p-Rb(S807/S811) | 173.37 | p-S6(S235/S236 | 412.91 | 2 | Colo858-signal2 | E7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 311.93 | p-Rb(S807/S811) | 166.54 | p-S6(S235/S236 | 387.41 | 2 | Colo858-signal2 | E8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 |