Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 421.06 | p-Rb(S807/S811) | 154.67 | p-ERK(T202/Y204) | 537.34 | 2 | Colo858-signal1 | G10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 447.88 | p-Rb(S807/S811) | 161.30 | p-ERK(T202/Y204) | 568.39 | 2 | Colo858-signal1 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1526.80 | p-Rb(S807/S811) | 101.09 | p-ERK(T202/Y204) | 29.24 | 2 | Colo858-signal1 | G2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 1676.60 | p-Rb(S807/S811) | 60.57 | p-ERK(T202/Y204) | 20.44 | 2 | Colo858-signal1 | G3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 1044.90 | p-Rb(S807/S811) | 48.21 | p-ERK(T202/Y204) | 22.38 | 2 | Colo858-signal1 | G4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 983.23 | p-Rb(S807/S811) | 56.72 | p-ERK(T202/Y204) | 28.24 | 2 | Colo858-signal1 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 743.36 | p-Rb(S807/S811) | 68.92 | p-ERK(T202/Y204) | 51.05 | 2 | Colo858-signal1 | G6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 458.83 | p-Rb(S807/S811) | 152.02 | p-ERK(T202/Y204) | 459.90 | 2 | Colo858-signal1 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 456.27 | p-Rb(S807/S811) | 159.28 | p-ERK(T202/Y204) | 556.01 | 2 | Colo858-signal1 | G9 |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 163.91 | p-Rb(S807/S811) | 54.08 | p-S6(S235/S236 | 23.01 | 1 | Colo858-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 165.33 | p-Rb(S807/S811) | 58.60 | p-S6(S235/S236 | 48.12 | 1 | Colo858-signal2 | B4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.45 | p-Rb(S807/S811) | 54.34 | p-S6(S235/S236 | 77.03 | 1 | Colo858-signal2 | B5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 204.59 | p-Rb(S807/S811) | 83.78 | p-S6(S235/S236 | 220.01 | 1 | Colo858-signal2 | B6 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 307.92 | p-Rb(S807/S811) | 162.01 | p-S6(S235/S236 | 403.15 | 1 | Colo858-signal2 | B8 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 341.90 | p-Rb(S807/S811) | 169.94 | p-S6(S235/S236 | 414.55 | 1 | Colo858-signal2 | B9 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 254.30 | p-Rb(S807/S811) | 163.98 | p-S6(S235/S236 | 398.31 | 2 | Colo858-signal2 | C10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 377.25 | p-Rb(S807/S811) | 161.02 | p-S6(S235/S236 | 380.19 | 2 | Colo858-signal2 | C11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 259.21 | p-Rb(S807/S811) | 68.62 | p-S6(S235/S236 | 16.39 | 2 | Colo858-signal2 | C2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 150.06 | p-Rb(S807/S811) | 51.74 | p-S6(S235/S236 | 21.56 | 2 | Colo858-signal2 | C3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 152.45 | p-Rb(S807/S811) | 55.46 | p-S6(S235/S236 | 57.68 | 2 | Colo858-signal2 | C4 |