Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 288.89 | p-Rb(S807/S811) | 152.75 | p-S6(S235/S236 | 379.48 | 1 | Colo858-signal2 | F10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 336.86 | p-Rb(S807/S811) | 161.69 | p-S6(S235/S236 | 384.38 | 1 | Colo858-signal2 | F11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 416.80 | p-Rb(S807/S811) | 107.61 | p-S6(S235/S236 | 19.41 | 1 | Colo858-signal2 | F2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 256.94 | p-Rb(S807/S811) | 69.35 | p-S6(S235/S236 | 26.20 | 1 | Colo858-signal2 | F3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 168.97 | p-Rb(S807/S811) | 57.67 | p-S6(S235/S236 | 47.25 | 1 | Colo858-signal2 | F4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 155.41 | p-Rb(S807/S811) | 59.98 | p-S6(S235/S236 | 99.31 | 1 | Colo858-signal2 | F5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 191.90 | p-Rb(S807/S811) | 81.85 | p-S6(S235/S236 | 199.11 | 1 | Colo858-signal2 | F6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 219.70 | p-Rb(S807/S811) | 143.66 | p-S6(S235/S236 | 371.59 | 1 | Colo858-signal2 | F7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 337.75 | p-Rb(S807/S811) | 156.22 | p-S6(S235/S236 | 357.37 | 1 | Colo858-signal2 | F8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 311.80 | p-Rb(S807/S811) | 160.43 | p-S6(S235/S236 | 378.06 | 1 | Colo858-signal2 | F9 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 300.67 | p-Rb(S807/S811) | 162.97 | p-S6(S235/S236 | 433.52 | 2 | Colo858-signal2 | G10 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 376.71 | p-Rb(S807/S811) | 165.48 | p-S6(S235/S236 | 397.13 | 2 | Colo858-signal2 | G11 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 525.79 | p-Rb(S807/S811) | 128.17 | p-S6(S235/S236 | 40.46 | 2 | Colo858-signal2 | G2 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 271.62 | p-Rb(S807/S811) | 67.91 | p-S6(S235/S236 | 39.37 | 2 | Colo858-signal2 | G3 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 164.14 | p-Rb(S807/S811) | 55.90 | p-S6(S235/S236 | 45.82 | 2 | Colo858-signal2 | G4 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 125.76 | p-Rb(S807/S811) | 58.82 | p-S6(S235/S236 | 110.32 | 2 | Colo858-signal2 | G5 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 194.38 | p-Rb(S807/S811) | 84.75 | p-S6(S235/S236 | 196.38 | 2 | Colo858-signal2 | G6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 239.08 | p-Rb(S807/S811) | 140.42 | p-S6(S235/S236 | 380.85 | 2 | Colo858-signal2 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 327.85 | p-Rb(S807/S811) | 162.48 | p-S6(S235/S236 | 410.06 | 2 | Colo858-signal2 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 343.94 | p-Rb(S807/S811) | 170.97 | p-S6(S235/S236 | 450.82 | 2 | Colo858-signal2 | G9 |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | Ki-67 | 2154.80 | p-Rb(S807/S811) | 145.55 | p-cJun(S73) | 28.64 | 1 | Colo858-signal3 | B10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | Ki-67 | 801.46 | p-Rb(S807/S811) | 44.01 | p-cJun(S73) | 45.24 | 1 | Colo858-signal3 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | Ki-67 | 772.11 | p-Rb(S807/S811) | 44.73 | p-cJun(S73) | 66.84 | 1 | Colo858-signal3 | B3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 |