Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
MMAC-SF 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 1.1751 2.1231 0.4854 0.2155 0.3125 0.6494 0.8696 0.5682 0.9434 0.9434 1.0695 0.6579 0.5465 0.6250 0.2353 0.6667 0.4608 0.4808 1.0526 0.8547
MMAC-SF 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 1.0000 1.6779 0.4184 0.2208 0.2347 0.5405 0.6757 0.4608 0.8265 0.8000 0.9009 0.6135 0.4525 0.5814 0.2110 0.7246 0.4695 0.3021 0.8772 0.7299
MMAC-SF 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 1.1038 1.7065 0.4292 0.2273 0.2646 0.6410 0.7752 0.5128 0.8547 0.8772 0.9524 0.6135 0.5128 0.5405 0.2320 0.6757 0.3378 0.9346 0.7246
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.1806 3.3445 0.5556 0.2545 0.3745 0.6410 1.0000 0.6211 1.3812 1.0718 1.3908 0.7463 0.2083 0.7092 0.4386 0.5848 1.1507 0.8403
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.1641 3.0211 0.5587 0.2564 0.3584 0.6098 0.8772 0.5650 1.3298 1.1211 1.3089 0.7353 0.5405 0.6849 0.2427 0.8130 0.4854 1.0977 0.7752
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.0256 2.0161 0.4566 0.2304 0.2732 0.5650 0.7407 0.5405 1.0858 0.9434 1.0341 0.6536 0.5051 0.6579 0.2299 0.7519 0.4505 0.3906 0.9901 0.7813
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.9901 1.9802 0.4546 0.2475 0.2874 0.5988 0.8130 0.5952 1.0438 0.9434 1.0352 0.5917 0.5208 0.6250 0.2174 0.7463 0.4630 0.3846 1.0267 0.7194
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.1261 2.7174 0.5076 0.2519 0.3257 0.6757 0.8265 0.5882 1.1521 0.9709 1.1933 0.6849 0.5525 0.6849 0.2299 0.6849 0.4608 0.4951 1.0173 0.8403
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.0764 2.5000 0.5435 0.2336 0.3215 0.7042 0.8696 0.5376 1.1574 0.9804 1.1325 0.6623 0.5495 0.6897 0.2320 0.7874 0.5076 0.4951 1.0235 0.7937
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.0881 2.2727 0.5000 0.2375 0.2899 0.6329 0.7752 0.5376 1.0989 0.8929 1.1351 0.6623 0.5348 0.6667 0.2247 0.7353 0.4739 0.4049 1.0870 0.7937
MMAC-SF 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 1.1587 2.3981 0.5263 0.2513 0.3425 0.6667 0.9174 0.5618 1.1364 0.9524 1.1442 0.6579 0.5556 0.6623 0.2387 0.7246 0.4608 0.4785 1.1074 0.7752
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 1.1628 3.0303 0.5128 0.2513 0.3584 0.6993 1.0000 1.2240 1.0953 1.2077 0.6711 0.5376 0.7143 0.2370 0.6803 0.4926 0.4854 0.9901 0.9434
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 1.0834 2.4938 0.5465 0.2416 0.3345 0.5650 0.8547 0.4651 1.0571 1.0183 1.1779 0.6061 0.5181 0.6494 0.2141 0.8197 0.5319 0.5181 1.0537 0.8475
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.9804 2.2523 0.4878 0.2283 0.2725 0.5263 0.8000 0.4673 0.9259 0.8547 1.0142 0.6135 0.4630 0.6211 0.2500 0.7299 0.4546 0.4167 0.8696 0.7299
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 1.0000 2.1978 0.5051 0.2398 0.3003 0.5618 0.9091 0.4608 0.9709 0.8696 1.0000 0.6289 0.4926 0.5952 0.2141 0.7407 0.4587 0.4292 0.9709 0.7813
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 1.0194 2.2989 0.5025 0.2370 0.3125 0.6369 0.8621 0.4695 0.9615 0.8850 1.0299 0.5650 0.4630 0.6369 0.2198 0.7092 0.4484 0.4464 0.8475 0.7813
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.9804 2.0747 0.4854 0.2212 0.2976 0.5682 0.8333 0.3953 0.9009 0.9009 1.0684 0.5780 0.4762 0.5814 0.2304 0.7519 0.4695 0.4219 0.9174 0.7692
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.8696 1.7153 0.4292 0.2169 0.2457 0.5650 0.6849 0.4310 0.7874 0.7937 0.8696 0.5525 0.4292 0.5587 0.2062 0.7634 0.4274 0.3300 0.8197 0.6667
MMAC-SF 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.9434 1.7212 0.4484 0.2315 0.2801 0.6024 0.7874 0.4255 0.8197 0.8547 0.8929 0.6494 0.4673 0.5618 0.2119 0.6993 0.4149 0.3788 0.9259 0.7092
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.1710 3.0120 0.5405 0.2597 0.3597 0.6211 0.9524 0.5618 1.2723 0.9804 1.1173 0.6536 0.5405 0.2174 0.6897 0.5076 0.5000 1.0010 0.9259
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.1628 2.6954 0.5405 0.2188 0.3460 0.5848 0.8772 0.4808 1.0331 0.9524 1.1614 0.7042 0.4878 0.6452 0.2160 0.7576 0.5025 0.5348 0.8265
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.1429 2.7027 0.5319 0.2451 0.3279 0.5682 0.8772 0.5917 1.1198 0.9434 1.0834 0.6757 0.4902 0.6289 0.2119 0.6944 0.5291 0.4695 0.9709 0.8265
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.2346 2.8011 0.5814 0.2370 0.3509 0.6211 0.9901 0.5814 0.9615 0.9709 1.1390 0.7143 0.5714 0.6289 0.2066 0.6993 0.4673 0.5181 1.0070 0.8265
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.1223 2.4213 0.4926 0.2092 0.3247 0.6757 0.8475 0.5525 1.0593 0.9346 1.0438 0.6289 0.5236 0.6135 0.2070 0.6757 0.4878 0.4386 0.9615 0.8547
MMAC-SF 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 1.1325 2.4272 0.4951 0.2227 0.3345 0.6757 0.8621 0.4854 0.8696 0.9346 1.0881 0.6711 0.5291 0.6329 0.2155 0.6667 0.4673 0.4762 1.0163 0.8333