Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0782 0.2268 0.0762 0.0134 0.0159 0.0310 0.0033 0.5882 0.0122 0.0346 0.2710 0.0280 0.0177 0.0047 0.0537 0.0133 0.0084 0.2283 0.1103
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0794 0.2169 0.0846 0.0100 0.0113 0.0283 0.0035 0.0084 0.5952 0.0136 0.0342 0.2994 0.0206 0.0045 0.0593 0.0152 0.0064 0.1733 0.1161
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0852 0.3333 0.0930 0.0217 0.0128 0.0356 0.0035 0.0068 0.7299 0.0200 0.0502 0.3155 0.0313 0.0291 0.0073 0.0641 0.0200 0.0091 0.2801 0.1057
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0842 0.3068 0.0899 0.0121 0.0121 0.0301 0.0037 0.0084 0.7576 0.0196 0.0473 0.2841 0.0323 0.0305 0.0055 0.0642 0.0129 0.0076 0.2227 0.0881
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0941 0.2688 0.0636 0.0103 0.0107 0.0346 0.0030 0.0082 0.6536 0.0165 0.0282 0.2809 0.0347 0.0391 0.0040 0.0638 0.0121 0.0079 0.2268 0.0895
LOXIMVI 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0889 0.1931 0.0702 0.0114 0.0114 0.0303 0.6173 0.0163 0.0281 0.3367 0.0317 0.0458 0.0046 0.0719 0.0127 0.0071 0.1799 0.1016
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0724 0.2639 0.0722 0.0128 0.0403 0.0030 0.0064 0.6803 0.0299 0.2985 0.0277 0.0207 0.0033 0.0787 0.0177 0.0078 0.2475 0.1185
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0746 0.2375 0.0758 0.0108 0.0117 0.0338 0.0035 0.0071 0.6211 0.0139 0.0267 0.2591 0.0224 0.0223 0.0035 0.0567 0.0111 0.0076 0.2193 0.0740
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0806 0.2632 0.0612 0.0105 0.0153 0.0349 0.0033 0.0052 0.6536 0.0160 0.0405 0.3906 0.0293 0.0287 0.0037 0.0673 0.0130 0.0075 0.2519 0.1701
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0890 0.2252 0.0722 0.0097 0.0177 0.0344 0.0037 0.5747 0.0148 0.0334 0.3300 0.0329 0.0044 0.0787 0.0068 0.2584 0.1613
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0829 0.2070 0.0657 0.0103 0.0161 0.0347 0.0030 0.0061 0.5714 0.0204 0.0294 0.3012 0.0323 0.0305 0.0038 0.0588 0.0114 0.0076 0.2488 0.1312
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0865 0.1923 0.0609 0.0092 0.0114 0.0296 0.0033 0.0071 0.5465 0.0197 0.0271 0.2801 0.0327 0.0292 0.0039 0.0595 0.0138 0.0062 0.2336 0.0856
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.1009 0.3165 0.0657 0.0109 0.0149 0.0324 0.0039 0.0052 0.8333 0.0173 0.0387 0.3333 0.0333 0.0342 0.0043 0.0763 0.0126 0.0096 0.2941
LOXIMVI 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.1111 0.3185 0.0759 0.0124 0.0143 0.0282 0.0047 0.0068 0.7042 0.0158 0.0343 0.3425 0.0346 0.0415 0.0784 0.0164 0.0086 0.2907 0.2020
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.1073 0.2310 0.1565 0.0230 0.0260 0.0370 0.0029 0.0166 0.9901 0.0383 0.0316 0.3745 0.0386 0.0273 0.0033 0.0816 0.0032 0.0092 0.2558 0.1658
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.1091 0.2294 0.1414 0.0239 0.0188 0.0270 0.0031 0.0062 0.9615 0.0945 0.0266 0.3155 0.0335 0.0271 0.0035 0.0677 0.0136 0.0088 0.2359 0.0940
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.1073 0.3226 0.0777 0.0161 0.0158 0.0329 0.0038 0.0069 0.9091 0.0209 0.0384 0.3953 0.0371 0.0463 0.0044 0.0726 0.0125 0.0088 0.3077 0.3086
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.1082 0.3175 0.0657 0.0127 0.0147 0.0249 0.0046 0.0071 0.9346 0.0201 0.0382 0.3367 0.0319 0.0439 0.0049 0.0668 0.0136 0.0087 0.2994 0.1724
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
LOXIMVI 0.0000
uM
 10
hr
 8
InnoScan/Mapix
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1326 0.1395 0.0723 0.0093 0.0110 0.0398 0.0030 0.0054 0.4310 0.0208 0.0257 0.3165 0.0328 0.0344 0.0046 0.0737 0.0126 0.0072 0.2933 0.1178
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1344 0.1916 0.0810 0.0109 0.0130 0.0383 0.0054 0.0095 0.4717 0.0261 0.0314 0.3460 0.0349 0.0379 0.0057 0.0781 0.0153 0.0078 0.3690 0.1093
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1377 0.1789 0.0818 0.0105 0.0105 0.0360 0.0032 0.0065 0.5000 0.0208 0.0384 0.2994 0.0354 0.0359 0.0046 0.0825 0.0120 0.0091 0.3185 0.1466