Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1451 0.1595 0.0844 0.0098 0.0131 0.0289 0.0038 0.0085 0.5155 0.0210 0.0387 0.3215 0.0400 0.0337 0.0055 0.0786 0.0131 0.0091 0.2985 0.1020
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1135 0.1277 0.0699 0.0080 0.0111 0.0398 0.0033 0.0058 0.3718 0.0141 0.0237 0.2899 0.0288 0.0273 0.0044 0.0659 0.0131 0.0074 0.2874 0.0974
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1175 0.1302 0.0714 0.0077 0.0142 0.0319 0.0039 0.0098 0.3953 0.0152 0.0300 0.3236 0.0312 0.0267 0.0053 0.0715 0.0147 0.0077 0.2755 0.0805
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1274 0.1639 0.0761 0.0091 0.0133 0.0375 0.0043 0.0082 0.4098 0.0142 0.0327 0.3984 0.0421 0.0314 0.0044 0.0926 0.0144 0.0099 0.3345 0.1618
LOXIMVI 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.1462 0.1362 0.0835 0.0096 0.0167 0.0348 0.0058 0.0087 0.4202 0.0152 0.0331 0.4484 0.0449 0.0292 0.0057 0.0947 0.0103 0.2985 0.1364
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1068 0.2096 0.1078 0.0112 0.0119 0.0387 0.0041 0.0075 0.5128 0.0225 0.0441 0.2849 0.0313 0.0308 0.0050 0.0708 0.0154 0.3891 0.0852
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1049 0.2227 0.1112 0.0119 0.0125 0.0356 0.0038 0.0084 0.5465 0.0235 0.0442 0.2725 0.0319 0.0349 0.0049 0.0707 0.0132 0.0079 0.3040 0.0621
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0975 0.1423 0.0792 0.0093 0.0098 0.0326 0.0027 0.0052 0.4237 0.0172 0.0281 0.2262 0.0262 0.0275 0.0042 0.0613 0.0097 0.0071 0.2571 0.0592
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0932 0.1299 0.0785 0.0094 0.0110 0.0287 0.0039 0.0078 0.4115 0.0174 0.0280 0.2513 0.0302 0.0350 0.0044 0.0642 0.0106 0.0060 0.2045 0.0630
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0790 0.1653 0.0854 0.0087 0.0099 0.0295 0.0029 0.0057 0.3937 0.0131 0.0352 0.2294 0.0241 0.0190 0.0047 0.0560 0.0118 0.0079 0.2985 0.0755
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0770 0.1543 0.0810 0.0089 0.0101 0.0265 0.0034 0.0075 0.4032 0.0135 0.0338 0.2222 0.0240 0.0188 0.0045 0.0577 0.0113 0.0061 0.2347 0.0654
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1044 0.1403 0.0673 0.0069 0.0114 0.0326 0.0026 0.0069 0.3509 0.0117 0.0256 0.2577 0.0303 0.0208 0.0036 0.0645 0.0111 0.0074 0.2193 0.0951
LOXIMVI 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1022 0.1033 0.0736 0.0083 0.0123 0.0309 0.0055 0.0107 0.3413 0.0147 0.0221 0.2924 0.0294 0.0192 0.0050 0.0762 0.0124 0.0079 0.1992 0.0973
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1037 0.2558 0.1126 0.0114 0.0187 0.0416 0.0048 0.0097 0.5291 0.0285 0.0550 0.3311 0.0320 0.0457 0.0047 0.0773 0.0181 0.0100 0.4202 0.0849
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1097 0.2416 0.1122 0.0116 0.0133 0.0375 0.0051 0.0112 0.4785 0.0255 0.0490 0.2933 0.0307 0.0492 0.0049 0.0779 0.0175 0.0081 0.3663 0.0626
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0919 0.1605 0.0845 0.0090 0.0116 0.0305 0.0027 0.0049 0.4739 0.0197 0.0330 0.2433 0.0227 0.0277 0.0037 0.0603 0.0113 0.0076 0.2762 0.0482
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0917 0.1502 0.0835 0.0101 0.0121 0.0263 0.0036 0.0079 0.4098 0.0211 0.0335 0.2392 0.0236 0.0331 0.0052 0.0597 0.0131 0.0065 0.2347 0.0491
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0970 0.2703 0.1149 0.0113 0.0161 0.0405 0.0039 0.0082 0.4587 0.0221 0.0526 0.3257 0.0309 0.0284 0.0047 0.0711 0.0184 0.0116 0.4000 0.0940
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1020 0.2439 0.1138 0.0133 0.0143 0.0348 0.0050 0.0119 0.4525 0.0198 0.0488 0.2976 0.0283 0.0299 0.0059 0.0745 0.0174 0.0087 0.3636 0.0794
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0809 0.1221 0.0669 0.0076 0.0106 0.0294 0.0028 0.0046 0.3704 0.0105 0.0254 0.2747 0.0239 0.0168 0.0041 0.0573 0.0100 0.0092 0.2660 0.0526
LOXIMVI 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0840 0.1174 0.0691 0.0090 0.0115 0.0285 0.0039 0.0074 0.2833 0.0141 0.0254 0.2299 0.0229 0.0196 0.0052 0.0570 0.0144 0.0069 0.2075 0.0634
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1166 0.2203 0.1221 0.0155 0.0180 0.0406 0.0036 0.0086 0.8130 0.0257 0.0468 0.3650 0.0347 0.0427 0.0044 0.0786 0.0162 0.0097 0.4566 0.1170
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1252 0.2227 0.1179 0.0133 0.0147 0.0324 0.0045 0.0104 0.6803 0.0251 0.0460 0.3049 0.0298 0.0468 0.0047 0.0733 0.0172 0.0103 0.4405 0.0693
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1075 0.1321 0.0774 0.0107 0.0121 0.0290 0.0026 0.0056 0.5465 0.0163 0.0279 0.2571 0.0247 0.0395 0.0033 0.0628 0.0106 0.0072 0.2725 0.0879
LOXIMVI 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1047 0.1230 0.0727 0.0101 0.0123 0.0244 0.0032 0.0086 0.4065 0.0165 0.0262 0.2320 0.0238 0.0325 0.0039 0.0645 0.0118 0.0072 0.2695 0.0646