Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0746 0.2924 0.0947 0.0166 0.0235 0.0418 0.0035 0.0094 1.0204 0.0166 0.0440 0.4630 0.0297 0.0231 0.0037 0.0670 0.0170 0.0098 0.2416 0.2110
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0792 0.2933 0.0884 0.0151 0.0203 0.0310 0.0041 0.0110 0.9709 0.0169 0.0498 0.3802 0.0271 0.0220 0.0045 0.0637 0.0184 0.0101 0.2481 0.0810
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0711 0.0660 0.0155 0.0160 0.0372 0.0032 0.0069 0.8065 0.0179 0.0390 0.4386 0.0316 0.0192 0.0039 0.0525 0.0140 0.0090 0.2128 0.1808
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0748 0.2793 0.0636 0.0116 0.0168 0.0246 0.0046 0.0104 0.6579 0.0180 0.0362 0.3125 0.0268 0.0172 0.0045 0.0513 0.0176 0.0096 0.2075 0.1012
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0687 0.2976 0.0733 0.0144 0.0185 0.0317 0.0039 0.0078 0.8130 0.0390 0.0546 0.3774 0.0264 0.0249 0.0040 0.0546 0.0154 0.0086 0.1795 0.2445
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0682 0.2817 0.0671 0.0118 0.0180 0.0231 0.0049 0.0098 0.7042 0.0127 0.0539 0.2732 0.0221 0.0265 0.0053 0.0494 0.0177 0.0099 0.1658 0.1287
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0672 0.2857 0.0637 0.0132 0.0160 0.0282 0.0048 0.0082 0.7937 0.0199 0.0615 0.4785 0.0298 0.0377 0.0042 0.0517 0.0164 0.0089 0.1822 0.2247
LOXIMVI 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0696 0.2660 0.0623 0.0166 0.0185 0.0222 0.0122 0.5814 0.0328 0.0561 0.3745 0.0236 0.0285 0.0054 0.0533 0.0214 0.0092 0.1789 0.1637
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1300 0.4386 0.1290 0.0184 0.0168 0.0560 0.0060 0.0130 0.9615 0.0221 0.0715 0.4505 0.0479 0.0404 0.0060 0.0786 0.0254 0.0124 0.3448 0.1984
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1374 0.4785 0.1389 0.0192 0.0250 0.0515 0.0164 1.0101 0.0277 0.0707 0.4329 0.0428 0.0440 0.0086 0.0845 0.0286 0.0129 0.3012 0.1191
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1060 0.2667 0.0931 0.0188 0.0172 0.0407 0.0042 0.0086 0.8065 0.0163 0.0507 0.3906 0.0396 0.0250 0.0054 0.0666 0.0152 0.0085 0.2506 0.1437
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0987 0.2427 0.1029 0.0138 0.0325 0.0044 0.0118 0.8065 0.0149 0.0494 0.4065 0.0384 0.0261 0.0680 0.0345 0.0080 0.2475 0.1181
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1067 0.3012 0.0907 0.0149 0.0105 0.0395 0.0038 0.0095 0.8333 0.0205 0.0461 0.3175 0.0374 0.0319 0.0057 0.0574 0.0151 0.0108 0.2924 0.1468
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1038 0.3003 0.0935 0.0123 0.0126 0.0336 0.0046 0.0118 0.9009 0.0231 0.0483 0.3175 0.0430 0.0422 0.0062 0.0620 0.0172 0.0087 0.2475 0.1014
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1153 0.2188 0.0835 0.0112 0.0110 0.0339 0.0041 0.0068 0.7576 0.0152 0.0498 0.3268 0.0429 0.0296 0.0045 0.0687 0.0110 0.0098 0.2762 0.2217
LOXIMVI 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.1103 0.2132 0.0663 0.0112 0.0113 0.0299 0.0048 0.0080 0.6944 0.0169 0.0435 0.3559 0.0430 0.0272 0.0060 0.0664 0.0148 0.0109 0.2353 0.2008
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1067 0.5405 0.1421 0.0158 0.0224 0.0518 0.0067 0.0124 0.9174 0.0232 0.0784 0.4132 0.0436 0.0402 0.0075 0.0767 0.0242 0.0140 0.3817 0.1531
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1041 0.5376 0.1439 0.0178 0.0206 0.0470 0.0064 0.0146 0.9346 0.0232 0.0746 0.3968 0.0432 0.0417 0.0061 0.0887 0.0211 0.0155 0.2916 0.1156
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0738 0.2591 0.0776 0.0118 0.0112 0.0383 0.0030 0.0061 0.6711 0.0184 0.0389 0.2688 0.0271 0.0212 0.0046 0.0532 0.0115 0.0083 0.2155 0.0985
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0685 0.2445 0.0790 0.0181 0.0115 0.0338 0.0042 0.0091 0.6329 0.0117 0.0382 0.2762 0.0272 0.0239 0.0051 0.0615 0.0133 0.0066 0.1667 0.1116
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1034 0.4902 0.1191 0.0171 0.0143 0.0473 0.0052 0.0103 0.9804 0.0280 0.0706 0.3831 0.0443 0.0499 0.0073 0.0705 0.0223 0.0126 0.3472 0.1304
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.1006 0.4717 0.1149 0.0154 0.0149 0.0406 0.0062 0.0139 0.8850 0.0284 0.0714 0.3831 0.0561 0.0468 0.0070 0.0752 0.0223 0.0098 0.2611 0.1220
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0890 0.2288 0.0660 0.0103 0.0097 0.0372 0.0029 0.0051 0.6536 0.0169 0.0323 0.2762 0.0359 0.0326 0.0036 0.0590 0.0101 0.0151 0.1980 0.1157
LOXIMVI 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0882 0.2041 0.0789 0.0118 0.0338 0.0057 0.0076 0.5848 0.0286 0.0299 0.3165 0.0618 0.0303 0.0092 0.0678 0.0332 0.0079 0.1802 0.1372
LOXIMVI 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.1040 0.5102 0.1274 0.0173 0.0475 0.0059 0.0148 0.9709 0.0333 0.0716 0.4405 0.0434 0.0491 0.0049 0.0793 0.0282 0.0136 0.3559 0.1541