Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0707 0.2695 0.0644 0.0114 0.0153 0.0248 0.5236 0.0134 0.0450 0.3937 0.0245 0.0286 0.0056 0.0521 0.0177 0.0099 0.1802 0.1822
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0974 0.5025 0.1333 0.0153 0.0283 0.0545 0.0073 0.0128 0.8130 0.0241 0.0782 0.4630 0.0442 0.0325 0.0065 0.0678 0.0377 0.0123 0.2336
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.1098 0.5263 0.1391 0.0182 0.0239 0.0479 0.0072 0.0138 0.8265 0.0270 0.0822 0.4292 0.0396 0.0344 0.0084 0.0707 0.0326 0.0131 0.2024 0.1692
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0760 0.2688 0.0805 0.0110 0.0136 0.0339 0.0034 0.0070 0.6623 0.0148 0.0473 0.3401 0.0276 0.0156 0.0044 0.0532 0.0132 0.0084 0.1689 0.1405
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0709 0.2604 0.0804 0.0118 0.0156 0.0293 0.0037 0.0086 0.7042 0.0153 0.0460 0.3623 0.0257 0.0170 0.0055 0.0576 0.0143 0.0071 0.1531 0.1087
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0891 0.3663 0.1011 0.0148 0.0174 0.0366 0.0055 0.0078 0.7937 0.0183 0.0627 0.3623 0.0329 0.0219 0.0071 0.0597 0.0219 0.0100 0.2169 0.2004
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0914 0.3367 0.1009 0.0143 0.0190 0.0347 0.0049 0.0099 0.8000 0.0192 0.0649 0.3333 0.0315 0.0239 0.0070 0.0591 0.0187 0.0166 0.1730 0.1341
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0710 0.2717 0.0642 0.0099 0.0123 0.0454 0.0030 0.0063 0.5682 0.0125 0.0461 0.2916 0.0345 0.0165 0.0039 0.0518 0.0122 0.0088 0.1862 0.1709
LOXIMVI 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0621 0.2525 0.0662 0.0110 0.0149 0.0311 0.0039 0.0079 0.5780 0.0113 0.0549 0.3096 0.0255 0.0173 0.0052 0.0528 0.0152 0.0102 0.1656 0.1647
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0822 0.5435 0.1351 0.0166 0.0281 0.0495 0.0063 0.0123 0.7813 0.0308 0.0777 0.4274 0.0404 0.0294 0.0061 0.0676 0.0265 0.0136 0.2786 0.1842
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0814 0.5102 0.1357 0.0187 0.0215 0.0542 0.0064 0.0120 0.8772 0.0241 0.0715 0.3937 0.0390 0.0289 0.0056 0.0669 0.0191 0.0100 0.2268 0.1227
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0573 0.3226 0.0680 0.0104 0.0143 0.0282 0.0032 0.0062 0.5618 0.0136 0.0405 0.2584 0.0232 0.0150 0.0041 0.0498 0.0109 0.0088 0.1629 0.1359
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0543 0.2941 0.0653 0.0190 0.0142 0.0289 0.0039 0.0083 0.5917 0.0138 0.0383 0.2625 0.0229 0.0173 0.0044 0.0584 0.0121 0.0068 0.1232 0.1466
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0778 0.4762 0.1223 0.0265 0.0219 0.0420 0.0057 0.0098 0.7194 0.0241 0.0758 0.4032 0.0359 0.0301 0.0095 0.0649 0.0234 0.0110 0.2310 0.1391
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0764 0.4444 0.1201 0.0147 0.0212 0.0392 0.0062 0.0119 0.7407 0.0213 0.0715 0.3650 0.0365 0.0321 0.0067 0.0664 0.0206 0.0107 0.1754 0.1227
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0576 0.2924 0.0626 0.0105 0.0137 0.0357 0.0039 0.0084 0.5405 0.0119 0.0489 0.2890 0.0236 0.0215 0.0039 0.0589 0.0120 0.0084 0.1497 0.1513
LOXIMVI 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0581 0.2941 0.0864 0.0157 0.0312 0.0316 0.0067 0.0118 0.5376 0.0118 0.0446 0.2874 0.0309 0.0202 0.0056 0.0620 0.0334 0.0086 0.1244 0.2208
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0728 0.4651 0.1104 0.0143 0.0429 0.0058 0.0105 0.7692 0.0216 0.0677 0.3937 0.0355 0.0273 0.0041 0.0594 0.0106 0.2421 0.1866
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0735 0.4219 0.1105 0.0143 0.0186 0.0380 0.0063 0.0111 0.7092 0.0213 0.0588 0.3425 0.0288 0.0279 0.0046 0.0584 0.0193 0.0096 0.2198 0.1059
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0534 0.3279 0.0660 0.0229 0.0195 0.0283 0.0032 0.0063 0.6211 0.0143 0.0416 0.2762 0.0214 0.0157 0.0035 0.0467 0.0131 0.0088 0.1692 0.1350
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0566 0.3096 0.0653 0.0107 0.0147 0.0241 0.0040 0.0089 0.5102 0.0168 0.0426 0.2674 0.0202 0.0186 0.0046 0.0481 0.0155 0.0077 0.1712 0.1126
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0680 0.3215 0.0902 0.0136 0.0207 0.0332 0.0038 0.0074 0.6944 0.0253 0.0539 0.3345 0.0254 0.0204 0.0044 0.0541 0.0174 0.0108 0.1845 0.1550
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0664 0.3012 0.0881 0.0294 0.0154 0.0283 0.0041 0.0082 0.6410 0.0155 0.0458 0.2786 0.0230 0.0222 0.0049 0.0535 0.0151 0.0074 0.1818 0.1038
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0555 0.2439 0.0603 0.0107 0.0197 0.0315 0.0031 0.0058 0.6250 0.0147 0.0482 0.2674 0.0268 0.0183 0.0034 0.0583 0.0121 0.0086 0.1745 0.1645
LOXIMVI 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0584 0.2747 0.0666 0.0104 0.0163 0.0249 0.0041 0.0089 0.4878 0.0120 0.0466 0.2786 0.0396 0.0210 0.0055 0.0480 0.0164 0.0082 0.1681 0.1745