Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.2967 0.0814 0.0160 0.0156 0.0353 0.0041 0.0062 0.7752 0.0150 0.3497 0.0356 0.0193 0.0045 0.0686 0.0119 0.0093 0.2105 0.2994
LOXIMVI 0.0000
uM
 5
hr
 1
InnoScan/Mapix
 0.0817 0.2525 0.0763 0.0158 0.0183 0.0378 0.0052 0.0075 0.6536 0.0148 0.0546 0.3788 0.0371 0.0197 0.0063 0.0734 0.0463 0.0110 0.1949 0.2410
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0691 0.4926 0.1134 0.0184 0.0201 0.0407 0.0047 0.0085 0.6711 0.0192 0.0721 0.3623 0.0338 0.0211 0.0055 0.0582 0.0216 0.2058 0.1629
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0680 0.4444 0.1178 0.0171 0.0191 0.0353 0.0053 0.0092 0.7092 0.0205 0.0696 0.3472 0.0356 0.0246 0.0049 0.0599 0.0183 0.0084 0.1585 0.1309
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0590 0.2577 0.0650 0.0104 0.0137 0.0308 0.0029 0.0053 0.5682 0.0160 0.0402 0.2591 0.0246 0.0146 0.0040 0.0478 0.0106 0.0072 0.1490 0.1282
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0593 0.2364 0.0709 0.0107 0.0149 0.0349 0.0040 0.0077 0.5682 0.0166 0.0380 0.3012 0.0264 0.0153 0.0049 0.0483 0.0151 0.0062 0.1155 0.1418
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0598 0.3425 0.0881 0.0189 0.0151 0.0317 0.0035 0.0057 0.5618 0.0160 0.0489 0.2841 0.0288 0.0204 0.0051 0.0498 0.0134 0.0094 0.1499 0.1368
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0583 0.2950 0.0832 0.0136 0.0148 0.0275 0.0043 0.0074 0.5882 0.0158 0.0470 0.2732 0.0271 0.0202 0.0049 0.0653 0.0133 0.0070 0.1166 0.1229
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0697 0.2433 0.0582 0.0098 0.0170 0.0333 0.0037 0.0059 0.5714 0.0405 0.2695 0.0277 0.0210 0.0033 0.0544 0.0117 0.0071 0.1261 0.2242
LOXIMVI 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0725 0.2079 0.0729 0.0110 0.0315 0.0067 0.0100 0.4926 0.0138 0.0399 0.3559 0.0219 0.0076 0.1172 0.1783
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0574 0.4000 0.1005 0.0140 0.0226 0.0358 0.0047 0.0093 0.6369 0.0185 0.0617 0.3378 0.0308 0.0220 0.0039 0.0540 0.0230 0.0105 0.2049 0.1898
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0634 0.3774 0.0983 0.0129 0.0161 0.0325 0.0056 0.0100 0.5848 0.0178 0.0560 0.2959 0.0271 0.0236 0.0050 0.0576 0.0199 0.0093 0.1822 0.1183
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0543 0.2551 0.0662 0.0102 0.0159 0.0267 0.0028 0.0055 0.5917 0.0120 0.0452 0.2577 0.0199 0.0145 0.0034 0.0466 0.0115 0.0081 0.1592 0.1302
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0552 0.2545 0.0657 0.0111 0.0159 0.0231 0.0041 0.0081 0.4546 0.0131 0.0402 0.2457 0.0193 0.0187 0.0051 0.0487 0.0157 0.0077 0.1511 0.1125
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0629 0.3378 0.0907 0.0128 0.0271 0.0321 0.0038 0.0072 0.6803 0.0544 0.3290 0.0347 0.0238 0.0039 0.0525 0.0166 0.0096 0.1876 0.1600
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0644 0.3040 0.0890 0.0147 0.0264 0.0287 0.0054 0.0098 0.5747 0.0233 0.0472 0.2933 0.0240 0.0259 0.0054 0.0569 0.0186 0.0080 0.1859 0.1047
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0537 0.2681 0.0590 0.0096 0.0151 0.0291 0.0030 0.0051 0.6024 0.0116 0.0475 0.2433 0.0217 0.0174 0.0032 0.0473 0.0107 0.0077 0.1629 0.1608
LOXIMVI 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0581 0.2933 0.0684 0.0121 0.0155 0.0269 0.0055 0.0077 0.4425 0.0132 0.0511 0.2571 0.0215 0.0227 0.0068 0.0190 0.0092 0.1681 0.1701
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0700 0.3390 0.1066 0.0173 0.0208 0.0371 0.0037 0.0080 1.0000 0.0167 0.0478 0.3953 0.0301 0.0191 0.0039 0.0582 0.0164 0.0093 0.2208 0.1873
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0799 0.3413 0.0952 0.0153 0.0192 0.0283 0.0047 0.0102 0.8403 0.0163 0.0459 0.3086 0.0275 0.0214 0.0086 0.0549 0.0227 0.0112 0.2128 0.0901
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0799 0.2907 0.0660 0.0118 0.0193 0.0316 0.0031 0.0061 0.7576 0.0117 0.0409 0.3731 0.0282 0.0194 0.0035 0.0494 0.0129 0.0079 0.1727 0.2041
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0802 0.2841 0.0598 0.0111 0.0187 0.0242 0.0038 0.0090 0.5556 0.0127 0.0347 0.2809 0.0228 0.0164 0.0044 0.0480 0.0150 0.0093 0.1905 0.1076
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0803 0.3906 0.1140 0.0192 0.0206 0.0324 0.0048 0.0087 0.9259 0.0194 0.0621 0.4115 0.0338 0.0298 0.0051 0.0564 0.0231 0.0108 0.2079 0.2128
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0799 0.3597 0.1006 0.0164 0.0204 0.0267 0.0053 0.0110 0.7407 0.0186 0.0548 0.3215 0.0268 0.0311 0.0062 0.0551 0.0229 0.0115 0.1992 0.1076
LOXIMVI 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0671 0.2667 0.0657 0.0128 0.0163 0.0266 0.0047 0.0097 0.7692 0.0478 0.3460 0.0270 0.0332 0.0037 0.0535 0.0115 0.0089 0.1880 0.2114