Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0826 0.4405 0.0113 0.0122 0.0158 0.1174 0.0245 0.1271 0.0439 0.0291 0.1650 0.0314 0.0265 0.0075 0.0597 0.0186 0.1058
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0929 0.4673 0.0141 0.0134 0.0131 0.1362 0.0285 0.1323 0.0458 0.0327 0.1832 0.0266 0.0311 0.0079 0.0544 0.0190 0.1186 0.0399
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0818 0.5917 0.0117 0.0109 0.0172 0.1518 0.0268 0.1362 0.0359 0.0337 0.2299 0.0428 0.0255 0.0088 0.0848 0.0223 0.1259 0.0408
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0890 0.6452 0.0135 0.0130 0.0151 0.1508 0.0274 0.1585 0.0374 0.0341 0.2336 0.0367 0.0329 0.0091 0.0844 0.0249 0.1242 0.0335
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0763 0.4525 0.0112 0.0119 0.0114 0.1068 0.0199 0.1215 0.0275 0.0276 0.1536 0.0264 0.0239 0.0064 0.0603 0.0172 0.0876 0.0470
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0873 0.5025 0.0147 0.0122 0.0168 0.1214 0.0239 0.1290 0.0260 0.0330 0.1707 0.0271 0.0257 0.0085 0.0389 0.0168 0.0912 0.0260
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0791 0.4386 0.0114 0.0095 0.0153 0.1522 0.0262 0.1248 0.0479 0.0307 0.2262 0.0392 0.0267 0.0068 0.0827 0.0192 0.1279 0.0328
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0853 0.4762 0.0111 0.0123 0.0143 0.1350 0.0257 0.0448 0.0307 0.2193 0.0356 0.0327 0.0082 0.0714 0.0200 0.1412 0.0261
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0822 0.4202 0.0098 0.0107 0.0128 0.1074 0.1214 0.0387 0.0253 0.1656 0.0300 0.0256 0.0074 0.0590 0.0188 0.1027 0.0360
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0842 0.4525 0.0112 0.0120 0.0182 0.1201 0.0255 0.1217 0.0379 0.0307 0.1764 0.0291 0.0288 0.0079 0.0533 0.0176 0.1075 0.0296
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0806 0.5556 0.0110 0.0106 0.0138 0.1353 0.0247 0.1205 0.0344 0.0293 0.2028 0.0373 0.0246 0.0076 0.0807 0.0220 0.1223 0.0303
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0906 0.5618 0.0107 0.0122 0.0143 0.1312 0.0265 0.1186 0.0351 0.0299 0.2183 0.0323 0.0278 0.0084 0.0779 0.0231 0.1353 0.0252
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0781 0.4717 0.0095 0.0118 0.0114 0.1025 0.0230 0.1142 0.0272 0.0250 0.1605 0.0296 0.0243 0.0074 0.0671 0.0212 0.1082 0.0318
WM1552C 0.0000
uM
 48
hr
 6
Odyssey/Microvigene
 0.0842 0.4651 0.0120 0.0122 0.0183 0.1245 0.0242 0.1274 0.0296 0.0316 0.1818 0.0275 0.0271 0.0077 0.0514 0.0190 0.1046 0.0284
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0697 0.4167 0.0108 0.0096 0.0135 0.1335 0.0227 0.1098 0.0434 0.0289 0.2092 0.0339 0.0272 0.0070 0.0700 0.0191 0.1114 0.0299
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0841 0.4367 0.0102 0.0136 0.0168 0.1191 0.0254 0.1017 0.0402 0.0280 0.2092 0.0323 0.0295 0.0072 0.0701 0.0213 0.1261 0.0251
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0712 0.3436 0.0106 0.0115 0.0123 0.0926 0.0190 0.1015 0.0233 0.0265 0.1217 0.0209 0.0252 0.0069 0.0396 0.0163 0.0725 0.0184
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0783 0.3460 0.0120 0.0125 0.0167 0.1001 0.0202 0.1078 0.0178 0.0284 0.1272 0.0201 0.0277 0.0077 0.0383 0.0156 0.0787 0.0170
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0720 0.5348 0.0112 0.0095 0.0136 0.1318 0.0231 0.1138 0.0302 0.0307 0.2045 0.0332 0.0246 0.0074 0.0784 0.0239 0.1002 0.0302
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0811 0.5556 0.0120 0.0118 0.0176 0.1357 0.0251 0.1168 0.0299 0.0303 0.2110 0.0309 0.0272 0.0086 0.0717 0.0221 0.1094 0.0268
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0724 0.4739 0.0094 0.0109 0.0120 0.0977 0.0199 0.1114 0.0224 0.0266 0.1520 0.0268 0.0248 0.0072 0.0484 0.0178 0.0865 0.0238
WM1552C 0.0000
uM
 48
hr
 7
Odyssey/Microvigene
 0.0822 0.4608 0.0113 0.0116 0.0123 0.1167 0.0212 0.1202 0.0211 0.0312 0.1613 0.0233 0.0252 0.0083 0.0468 0.0185 0.0810 0.0258
WM1552C 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.0736 0.3300 0.0095 0.0101 0.0143 0.0931 0.0173 0.1206 0.0197 0.0256 0.1129 0.0211 0.0269 0.0069 0.0373 0.0148 0.0701 0.0167
WM1552C 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.0794 0.3623 0.0101 0.0111 0.0140 0.0885 0.0139 0.1114 0.0121 0.0241 0.1203 0.0216 0.0280 0.0076 0.0383 0.0142 0.0701 0.0128
WM1552C 0.0000
uM
 48
hr
 8
Odyssey/Microvigene
 0.0740 0.3817 0.0089 0.0109 0.0120 0.0884 0.0166 0.1130 0.0158 0.0253 0.1160 0.0201 0.0263 0.0073 0.0361 0.0141 0.0643 0.0133