Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0890 0.5155 0.0122 0.0123 0.0136 0.1335 0.0279 0.1151 0.0477 0.0301 0.2075 0.0366 0.0322 0.0077 0.0698 0.0200 0.1287 0.0296
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0817 0.4878 0.0107 0.0116 0.0127 0.1208 0.0260 0.1259 0.0416 0.0279 0.1658 0.0329 0.0284 0.0073 0.0565 0.0206 0.1063 0.0452
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0887 0.4975 0.0124 0.0123 0.0177 0.1357 0.0277 0.1383 0.0431 0.0320 0.1938 0.0298 0.0314 0.0087 0.0558 0.0179 0.1093 0.0338
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0798 0.5952 0.0107 0.0120 0.0129 0.1506 0.0265 0.1335 0.0351 0.0300 0.2310 0.0408 0.0254 0.0075 0.0994 0.0275 0.1290 0.0356
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0869 0.6173 0.0128 0.0124 0.0185 0.1482 0.0300 0.1266 0.0340 0.0350 0.2439 0.0389 0.0294 0.0079 0.1012 0.0251 0.1314 0.0310
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0751 0.5128 0.0099 0.0118 0.0124 0.1093 0.0231 0.1157 0.0279 0.0275 0.1712 0.0274 0.0237 0.0064 0.0608 0.0217 0.1130 0.0352
WM1552C 0.0000
uM
 48
hr
 2
Odyssey/Microvigene
 0.0809 0.5495 0.0127 0.0118 0.0138 0.1377 0.0240 0.1425 0.0307 0.0365 0.1969 0.0322 0.0253 0.0085 0.0584 0.0217 0.1031 0.0286
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0776 0.5208 0.0128 0.0096 0.0145 0.1536 0.0266 0.1318 0.0461 0.0324 0.2268 0.0395 0.0298 0.0083 0.0817 0.0219 0.1078 0.0287
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0861 0.5102 0.0115 0.0113 0.0185 0.1410 0.0287 0.1227 0.0443 0.0308 0.2375 0.0413 0.0325 0.0079 0.0776 0.0258 0.1235 0.0273
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0747 0.4237 0.0107 0.0125 0.1277 0.0250 0.1198 0.0391 0.0285 0.1890 0.0344 0.0282 0.0076 0.0699 0.0250 0.1109 0.0311
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0834 0.4329 0.0126 0.0116 0.0177 0.1326 0.0263 0.1279 0.0396 0.0315 0.2058 0.0307 0.0295 0.0083 0.0654 0.0202 0.1153 0.0308
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0776 0.6289 0.0117 0.0106 0.0150 0.1520 0.0264 0.1250 0.0341 0.0314 0.2227 0.0384 0.0260 0.0081 0.1020 0.0232 0.1201 0.0303
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0867 0.6061 0.0112 0.0127 0.0182 0.1524 0.0284 0.1311 0.0327 0.0344 0.2404 0.0360 0.0280 0.0085 0.0915 0.0232 0.1287 0.0298
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0745 0.5076 0.0101 0.0121 0.0123 0.1041 0.0233 0.1151 0.0249 0.0253 0.1686 0.0313 0.0226 0.0072 0.0535 0.0175 0.1048 0.0290
WM1552C 0.0000
uM
 48
hr
 3
Odyssey/Microvigene
 0.0815 0.4831 0.0121 0.0115 0.0155 0.1351 0.0252 0.1387 0.0291 0.0348 0.1880 0.0294 0.0253 0.0093 0.0531 0.0195 0.0970 0.0281
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0760 0.4854 0.0124 0.0093 0.0182 0.1445 0.0239 0.1177 0.0459 0.0322 0.2083 0.0359 0.0285 0.0074 0.0809 0.0209 0.0943 0.0328
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0851 0.4926 0.0114 0.0108 0.0172 0.1364 0.0269 0.1075 0.0448 0.0299 0.2137 0.0332 0.0313 0.0072 0.0740 0.0221 0.1164 0.0258
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0739 0.4219 0.0088 0.0107 0.0115 0.1024 0.0207 0.1117 0.0313 0.0267 0.1439 0.0283 0.0258 0.0070 0.0484 0.0162 0.0877 0.0301
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0812 0.3922 0.0104 0.0109 0.0157 0.1117 0.0225 0.1110 0.0304 0.0287 0.1423 0.0274 0.0266 0.0080 0.0513 0.0163 0.0907 0.0284
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0762 0.6024 0.0107 0.0094 0.0134 0.1555 0.0253 0.1171 0.0330 0.0327 0.2304 0.0408 0.0264 0.0075 0.0997 0.0223 0.1082 0.0271
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0883 0.5780 0.0119 0.0108 0.0180 0.1587 0.0265 0.1178 0.0315 0.0323 0.2381 0.0358 0.0294 0.0090 0.0834 0.0258 0.1181 0.0254
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0748 0.5405 0.0101 0.0099 0.0122 0.1203 0.0228 0.1094 0.0268 0.0272 0.1815 0.0261 0.0240 0.0070 0.0656 0.0182 0.1214 0.0273
WM1552C 0.0000
uM
 48
hr
 4
Odyssey/Microvigene
 0.0810 0.5263 0.0127 0.0114 0.0134 0.1460 0.0260 0.0280 0.0350 0.2012 0.0303 0.0205 0.0089 0.0639 0.0211 0.1112 0.0293
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0748 0.4785 0.0117 0.0098 0.0129 0.1518 0.0271 0.1152 0.0466 0.0284 0.2237 0.0384 0.0260 0.0074 0.0646 0.0174 0.1323 0.0454
WM1552C 0.0000
uM
 48
hr
 5
Odyssey/Microvigene
 0.0845 0.5263 0.0116 0.0131 0.0186 0.1427 0.0258 0.1490 0.0508 0.0328 0.2188 0.0357 0.0366 0.0090 0.0791 0.0237 0.1323 0.0352