Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0908 0.8130 0.0151 0.0150 0.0217 0.2160 0.0424 0.2551 0.0449 0.0355 0.4049 0.0397 0.0334 0.0088 0.1062 0.0233 0.1684 0.0239
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0861 0.6711 0.0117 0.0144 0.0213 0.1669 0.0302 0.2174 0.0392 0.0330 0.3311 0.0352 0.0284 0.0079 0.0953 0.0233 0.1236 0.0339
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0922 0.7692 0.0159 0.0135 0.0292 0.1931 0.0324 0.2463 0.0399 0.0372 0.3774 0.0324 0.0341 0.0092 0.0696 0.0219 0.1199 0.0237
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0779 0.7692 0.0110 0.0118 0.0179 0.1996 0.0371 0.2392 0.0335 0.0343 0.3922 0.0432 0.0280 0.0084 0.1120 0.0174 0.1374 0.0217
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0833 0.8065 0.0122 0.0126 0.0163 0.1733 0.0371 0.1799 0.0322 0.0336 0.4115 0.0411 0.0333 0.0092 0.1056 0.0200 0.1536 0.0198
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0691 0.6579 0.0103 0.0130 0.0156 0.1407 0.0303 0.2169 0.0284 0.0295 0.2618 0.0327 0.0295 0.0082 0.0543 0.0172 0.1024 0.0240
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0817 0.7092 0.0117 0.0138 0.0208 0.1407 0.0324 0.2212 0.0265 0.0319 0.2890 0.0288 0.0305 0.0077 0.0532 0.0138 0.1094 0.0200
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0717 0.6849 0.0130 0.0115 0.0230 0.2242 0.0331 0.2611 0.0449 0.0339 0.4630 0.0478 0.0271 0.0071 0.1258 0.0227 0.1894 0.0250
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0777 0.6993 0.0126 0.0149 0.0273 0.1946 0.0371 0.2551 0.0426 0.0345 0.5102 0.0456 0.0292 0.0081 0.1218 0.0237 0.2033 0.0243
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0812 0.6579 0.0112 0.0131 0.0207 0.1808 0.0318 0.2427 0.0402 0.0287 0.4219 0.0406 0.0257 0.0079 0.1038 0.0238 0.1898 0.0265
WM1552C 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.0847 0.6993 0.0127 0.0119 0.0277 0.2088 0.0336 0.2577 0.0438 0.0370 0.4630 0.0325 0.0300 0.0084 0.0956 0.0225 0.1818 0.0242
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0746 0.7246 0.0130 0.0120 0.0180 0.1898 0.0340 0.2416 0.0303 0.0343 0.3759 0.0409 0.0294 0.0077 0.0818 0.0176 0.1304 0.0237
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0835 0.7813 0.0107 0.0139 0.0230 0.1701 0.0360 0.2137 0.0299 0.0321 0.3788 0.0381 0.0341 0.0079 0.0856 0.0215 0.1511 0.0222
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0741 0.6579 0.0104 0.0117 0.0151 0.1391 0.0279 0.2123 0.0264 0.0299 0.2809 0.0328 0.0271 0.0078 0.0603 0.0156 0.1179 0.0240
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0820 0.6536 0.0120 0.0132 0.0195 0.1548 0.0308 0.2193 0.0255 0.0304 0.3068 0.0273 0.0288 0.0088 0.0649 0.0182 0.1178 0.0238
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0772 0.7092 0.0132 0.0128 0.0228 0.1988 0.0331 0.2273 0.0404 0.0360 0.3861 0.0407 0.0273 0.0085 0.1125 0.0249 0.1348 0.0248
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0842 0.7143 0.0124 0.0135 0.0279 0.1862 0.0360 0.2252 0.0398 0.0361 0.4065 0.0365 0.0318 0.0090 0.0961 0.0242 0.1441 0.0243
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0716 0.5714 0.0103 0.0116 0.0183 0.1464 0.0273 0.1946 0.0310 0.0279 0.2933 0.0289 0.0251 0.0081 0.0653 0.0159 0.1146 0.0219
WM1552C 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0779 0.5952 0.0126 0.0130 0.0211 0.1695 0.0277 0.2247 0.0339 0.0332 0.3135 0.0260 0.0251 0.0091 0.0597 0.0184 0.1148 0.0253
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0722 0.6173 0.0106 0.0109 0.0186 0.1198 0.0245 0.2257 0.0203 0.0296 0.2217 0.0251 0.0264 0.0072 0.0407 0.0096 0.0708 0.0267
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0793 0.6250 0.0104 0.0123 0.0164 0.1087 0.0201 0.1969 0.0174 0.0262 0.2193 0.0228 0.0263 0.0070 0.0367 0.0113 0.0708 0.0188
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0730 0.6098 0.0098 0.0117 0.0132 0.1085 0.0197 0.2222 0.0145 0.0288 0.1972 0.0202 0.0265 0.0073 0.0338 0.0103 0.0680 0.0184
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0810 0.5587 0.0106 0.0128 0.0178 0.1119 0.0205 0.2183 0.0135 0.0303 0.2058 0.0192 0.0276 0.0078 0.0409 0.0108 0.0726 0.0150
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0668 0.4587 0.0094 0.0107 0.0212 0.0966 0.0189 0.1828 0.0192 0.0260 0.1783 0.0206 0.0210 0.0058 0.0324 0.0095 0.0639 0.0175
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0723 0.4425 0.0094 0.0128 0.0200 0.0999 0.0172 0.1653 0.0170 0.0258 0.1855 0.0189 0.0223 0.0066 0.0330 0.0092 0.0641 0.0135