Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0664 0.4651 0.0091 0.0107 0.0161 0.0866 0.0173 0.1647 0.0102 0.0254 0.1383 0.0160 0.0223 0.0062 0.0263 0.0081 0.0610 0.0122
WM1552C 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0752 0.4255 0.0099 0.0114 0.0164 0.0912 0.1894 0.0097 0.0264 0.1447 0.0180 0.0206 0.0071 0.0250 0.0095 0.0507 0.0112
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0815 0.4149 0.0094 0.0111 0.0102 0.0941 0.0174 0.1341 0.0289 0.0247 0.1217 0.0167 0.0394 0.0069 0.0298 0.0099 0.0625 0.0266
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0853 0.4386 0.0115 0.0142 0.0123 0.0905 0.0164 0.1661 0.0213 0.0258 0.1306 0.0159 0.0482 0.0085 0.0296 0.0109 0.0625 0.0175
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.1002 0.5208 0.0106 0.0132 0.0150 0.1441 0.0258 0.1779 0.0423 0.0298 0.2119 0.0278 0.0726 0.0076 0.0600 0.0135 0.1078 0.0433
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.1131 0.5556 0.0127 0.0135 0.0119 0.1421 0.0286 0.1848 0.0402 0.0324 0.2208 0.0291 0.0856 0.0087 0.0604 0.0168 0.1156 0.0320
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0996 0.4184 0.0105 0.0134 0.0099 0.0845 0.0181 0.1423 0.0211 0.0265 0.1250 0.0153 0.0631 0.0076 0.0394 0.0135 0.0697 0.0193
WM1552C 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.1068 0.4739 0.0133 0.0122 0.0117 0.1005 0.0176 0.1689 0.0193 0.0308 0.1272 0.0173 0.0657 0.0089 0.0317 0.0125 0.0641 0.0143
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0915 0.5465 0.0113 0.0122 0.0138 0.1736 0.0274 0.1733 0.0594 0.0307 0.2907 0.0337 0.0522 0.0083 0.0842 0.0206 0.1297 0.0302
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0976 0.5348 0.0126 0.0154 0.0128 0.1531 0.0284 0.1541 0.0574 0.0306 0.2882 0.0327 0.0622 0.0081 0.0760 0.0190 0.1439 0.0253
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0873 0.5076 0.0102 0.0129 0.0117 0.1287 0.0251 0.1661 0.0481 0.0266 0.2155 0.0250 0.0473 0.0076 0.0554 0.0169 0.1133 0.0338
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0935 0.5405 0.0131 0.0137 0.0178 0.1389 0.0272 0.1812 0.0477 0.0301 0.2320 0.0212 0.0496 0.0083 0.0602 0.0179 0.1117 0.0262
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.1062 0.5814 0.0122 0.0128 0.0122 0.1835 0.0281 0.1748 0.0475 0.0319 0.2890 0.0362 0.0774 0.0091 0.1133 0.0259 0.1439 0.0297
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.1082 0.6098 0.0136 0.0138 0.0172 0.1733 0.1792 0.0474 0.0353 0.2959 0.0351 0.0943 0.0092 0.0918 0.0229 0.1427 0.0284
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.1013 0.5465 0.0099 0.0120 0.0128 0.1346 0.0273 0.1637 0.0387 0.0291 0.2304 0.0242 0.0677 0.0076 0.0779 0.0208 0.1199 0.0328
WM1552C 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.1040 0.5848 0.0134 0.0117 0.0157 0.1631 0.0263 0.0418 0.2577 0.0285 0.0733 0.0096 0.0598 0.0190 0.1112 0.0270
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0852 0.5000 0.0129 0.0125 0.0139 0.1698 0.0259 0.1664 0.0550 0.0309 0.2762 0.0335 0.0501 0.0079 0.0750 0.0170 0.1337 0.0284
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0912 0.5291 0.0113 0.0145 0.0185 0.1506 0.0283 0.1580 0.0527 0.0309 0.2809 0.0313 0.0551 0.0088 0.0730 0.0222 0.1495 0.0265
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0818 0.4831 0.0100 0.0117 0.0118 0.1134 0.0196 0.1449 0.0371 0.0264 0.1704 0.0191 0.0431 0.0072 0.0480 0.0154 0.0868 0.0263
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0871 0.4717 0.0121 0.0129 0.0169 0.1229 0.0233 0.1531 0.0344 0.0287 0.1802 0.0177 0.0444 0.0077 0.0456 0.0150 0.0881 0.0237
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0977 0.5556 0.0126 0.0125 0.0126 0.1698 0.0269 0.1495 0.0439 0.0324 0.2525 0.0338 0.0737 0.0084 0.0963 0.0195 0.1269
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.1075 0.5556 0.0125 0.0135 0.0180 0.1608 0.0281 0.1565 0.0435 0.0358 0.2667 0.0295 0.0759 0.0082 0.0863 0.0207 0.1326 0.0292
WM1552C 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0948 0.4785 0.0099 0.0136 0.0116 0.1098 0.0219 0.1405 0.0324 0.0267 0.1792 0.0263 0.0633 0.0080 0.0556 0.0138 0.1075 0.0279