Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0831 0.7692 0.0129 0.0146 0.0211 0.2128 0.0383 0.2433 0.0441 0.0353 0.3953 0.0467 0.0303 0.0084 0.1239 0.0264 0.1490 0.0329
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0932 0.8333 0.0135 0.0154 0.0270 0.2041 0.0421 0.2288 0.0455 0.0394 0.4184 0.0428 0.0332 0.0088 0.0912 0.0242 0.1518 0.0293
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0817 0.6757 0.0117 0.0129 0.0198 0.1534 0.0321 0.2119 0.0351 0.0289 0.3077 0.0290 0.0271 0.0074 0.0711 0.0212 0.1182 0.0307
WM1552C 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0896 0.7246 0.0141 0.0137 0.0231 0.1845 0.0328 0.2632 0.0386 0.0392 0.3460 0.0346 0.0306 0.0095 0.0618 0.0208 0.1098 0.0236
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0789 0.8065 0.0139 0.0118 0.0188 0.2079 0.0356 0.2551 0.0306 0.0366 0.4098 0.0483 0.0309 0.0076 0.0999 0.0259 0.1482 0.0214
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0868 0.8621 0.0130 0.0137 0.0235 0.1852 0.0383 0.2427 0.0307 0.0364 0.4115 0.0435 0.0355 0.0097 0.1120 0.0228 0.1692 0.0225
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0803 0.7407 0.0101 0.0119 0.0149 0.1631 0.0331 0.2217 0.0305 0.0322 0.3345 0.0390 0.0328 0.0078 0.1188 0.0188 0.1585 0.0212
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0848 0.7634 0.0127 0.0134 0.0225 0.1748 0.0368 0.2457 0.0315 0.0375 0.3534 0.0359 0.0335 0.0089 0.0945 0.0235 0.1567 0.0218
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0813 0.7519 0.0141 0.0139 0.0203 0.2146 0.0353 0.2494 0.0406 0.0358 0.4219 0.0484 0.0307 0.0087 0.1332 0.0304 0.1718 0.0256
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0926 0.7634 0.0131 0.0153 0.0290 0.2062 0.0367 0.2538 0.0375 0.0374 0.4274 0.0438 0.0318 0.0094 0.1359 0.0240 0.1795 0.0263
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0793 0.6579 0.0100 0.0124 0.0179 0.1608 0.0307 0.2165 0.0372 0.0301 0.3401 0.0372 0.0273 0.0092 0.0901 0.0151 0.1397 0.0224
WM1552C 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0865 0.6494 0.0131 0.0130 0.0200 0.1996 0.0325 0.2618 0.0414 0.0395 0.3704 0.0331 0.0289 0.0092 0.0890 0.0255 0.1330 0.0232
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0720 0.6452 0.0094 0.0119 0.0203 0.1341 0.0277 0.1859 0.0237 0.0342 0.2247 0.0302 0.0267 0.0062 0.0483 0.0121 0.0797 0.0285
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0788 0.6024 0.0106 0.0137 0.0182 0.1264 0.0279 0.1689 0.0205 0.0315 0.2058 0.0292 0.0289 0.0079 0.0364 0.0121 0.0840 0.0224
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0726 0.6329 0.0101 0.0121 0.0138 0.1029 0.0216 0.1692 0.0169 0.0295 0.1639 0.0225 0.0270 0.0075 0.0338 0.0110 0.0698 0.0194
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0797 0.5682 0.0113 0.0133 0.0174 0.1125 0.0214 0.1799 0.0164 0.0324 0.1600 0.0214 0.0267 0.0072 0.0322 0.0122 0.0670 0.0178
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0826 0.8130 0.0171 0.0135 0.0236 0.2336 0.0384 0.2451 0.0460 0.0407 0.4202 0.0501 0.0321 0.0085 0.1541 0.0257 0.1490 0.0294
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0955 0.7299 0.0147 0.0144 0.0292 0.2268 0.0377 0.2262 0.0440 0.0427 0.4367 0.0418 0.0100 0.1372 0.0309 0.1529 0.0278
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0791 0.6849 0.0122 0.0115 0.0189 0.1613 0.0311 0.2028 0.0376 0.0331 0.3195 0.0293 0.0292 0.0081 0.0759 0.0148 0.1206 0.0218
WM1552C 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0885 0.7042 0.0156 0.0137 0.0215 0.2041 0.0364 0.2695 0.0400 0.0453 0.3460 0.0342 0.0243 0.0102 0.0726 0.0215 0.1217 0.0264
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0754 0.7246 0.0129 0.0116 0.0170 0.1736 0.0349 0.2247 0.0313 0.0302 0.3521 0.0420 0.0257 0.0074 0.0700 0.0164 0.1258 0.0264
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0828 0.8000 0.0133 0.0146 0.0198 0.1645 0.0283 0.2299 0.0302 0.0332 0.3584 0.0379 0.0354 0.0086 0.0805 0.0188 0.1289 0.0238
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0837 0.6757 0.0118 0.0150 0.0185 0.1223 0.0288 0.2257 0.0265 0.0319 0.2342 0.0299 0.0266 0.0079 0.0557 0.0152 0.1088
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0898 0.7194 0.0122 0.0157 0.0160 0.1403 0.0315 0.2488 0.0258 0.0338 0.2463 0.0278 0.0312 0.0079 0.0444 0.0135 0.1136 0.0231
WM1552C 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.0851 0.7299 0.0135 0.0134 0.0257 0.2066 0.0357 0.2257 0.0471 0.0343 0.4000 0.0489 0.0271 0.0093 0.1023 0.0174 0.1610 0.0282