Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0238 0.1468 0.0255 0.0103 0.0033 0.0036 0.0025 0.0035 0.0034 0.0977 0.0359 0.0216 0.0324 0.0062 0.0043 0.0035 0.0046 0.0039 0.0043 0.0673 0.0358
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0297 0.1835 0.0266 0.0126 0.0043 0.0053 0.0029 0.0038 0.0041 0.0788 0.0410 0.0204 0.0466 0.0065 0.0046 0.0037 0.0055 0.0063 0.0048 0.0747 0.0554
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0241 0.2283 0.0494 0.0238 0.0084 0.0046 0.0040 0.0056 0.0046 0.0314 0.0429 0.0238 0.0495 0.0108 0.0053 0.0064 0.0068 0.0075 0.0051 0.0783 0.0335
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0323 0.2519 0.0518 0.0294 0.0089 0.0064 0.0040 0.0052 0.0053 0.0342 0.0526 0.0233 0.0680 0.0106 0.0051 0.0060 0.0082 0.0088 0.0056 0.0832 0.0486
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0277 0.1597 0.0244 0.0136 0.0035 0.0036 0.0024 0.0036 0.0038 0.1242 0.0298 0.0163 0.0354 0.0088 0.0049 0.0047 0.0051 0.0045 0.0049 0.0762 0.0403
WM1552C 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0388 0.2151 0.0359 0.0186 0.0052 0.0065 0.0029 0.0080 0.0056 0.0917 0.0441 0.0193 0.0591 0.0100 0.0088 0.0075 0.0050 0.0093 0.0055 0.0863 0.0619
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0240 0.2020 0.0413 0.0190 0.0084 0.0051 0.0038 0.0048 0.0049 0.0493 0.0447 0.0198 0.0494 0.0103 0.0051 0.0047 0.0068 0.0087 0.0058 0.0741 0.0335
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0333 0.2375 0.0411 0.0225 0.0078 0.0063 0.0038 0.0043 0.0048 0.0601 0.0536 0.0171 0.0604 0.0106 0.0055 0.0041 0.0072 0.0087 0.0064 0.0796 0.0455
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0353 0.3817 0.0745 0.0469 0.0109 0.0089 0.0062 0.0082 0.0075 0.0320 0.0745 0.0349 0.0812 0.0226 0.0081 0.0096 0.0101 0.0137 0.0079 0.1172 0.0548
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.4386 0.0793 0.0604 0.0109 0.0101 0.0059 0.0076 0.0083 0.0342 0.0912 0.0337 0.1185 0.0225 0.0077 0.0087 0.0105 0.0176 0.0089 0.1229 0.0749
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0313 0.1949 0.0353 0.0206 0.0049 0.0052 0.0029 0.0044 0.0051 0.0466 0.0366 0.0193 0.0439 0.0095 0.0052 0.0045 0.0061 0.0063 0.0053 0.0788 0.0413
WM1552C 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0364 0.2169 0.0448 0.0234 0.0054 0.0064 0.0029 0.0040 0.0057 0.0360 0.0498 0.0237 0.0658 0.0102 0.0054 0.0053 0.0062 0.0106 0.0056 0.0860 0.0592
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0344 0.4082 0.0831 0.0369 0.0131 0.0128 0.0077 0.0098 0.0117 0.0319 0.1100 0.0425 0.1073 0.0222 0.0107 0.0084 0.0151 0.0238 0.0111 0.1217 0.0640
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0491 0.5291 0.0841 0.0476 0.0149 0.0139 0.0077 0.0113 0.0118 0.0320 0.1462 0.0455 0.1592 0.0264 0.0079 0.0092 0.0129 0.0285 0.0100 0.1669 0.0807
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0265 0.2165 0.0520 0.0219 0.0083 0.0069 0.0056 0.0056 0.0060 0.0255 0.0554 0.0217 0.0590 0.0119 0.0059 0.0048 0.0073 0.0115 0.0058 0.0740 0.0414
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0300 0.3322 0.0578 0.0245 0.0082 0.0072 0.0036 0.0060 0.0058 0.0257 0.0733 0.0238 0.0850 0.0102 0.0049 0.0044 0.0074 0.0114 0.0052 0.0881 0.0455
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0418 0.5051 0.0828 0.0423 0.0224 0.0143 0.0085 0.0139 0.0121 0.0283 0.1079 0.0464 0.1233 0.0257 0.0122 0.0112 0.0179 0.0506 0.0120 0.1541 0.0811
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0549 0.6410 0.1133 0.0561 0.0243 0.0125 0.0070 0.0129 0.0121 0.0327 0.1387 0.0548 0.1764 0.0253 0.0093 0.0116 0.0140 0.0307 0.0124 0.1916 0.0963
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0296 0.1873 0.0362 0.0173 0.0049 0.0041 0.0038 0.0046 0.0048 0.0397 0.0360 0.0187 0.0466 0.0090 0.0055 0.0047 0.0067 0.0068 0.0070 0.0836 0.0461
WM1552C 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0324 0.2278 0.0359 0.0167 0.0049 0.0050 0.0033 0.0049 0.0059 0.0425 0.0462 0.0238 0.0615 0.0084 0.0048 0.0052 0.0065 0.0082 0.0054 0.0890 0.0528
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0330 0.3906 0.0775 0.0354 0.0140 0.0116 0.0070 0.0114 0.0105 0.0301 0.1062 0.0375 0.1016 0.0222 0.0095 0.0055 0.0108 0.0206 0.0102 0.1131 0.0585
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0398 0.5025 0.0769 0.0387 0.0161 0.0113 0.0071 0.0092 0.0080 0.0325 0.1192 0.0398 0.1316 0.0203 0.0100 0.0055 0.0128 0.0195 0.0105 0.1267 0.0676
WM1552C 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0234 0.1957 0.0436 0.0199 0.0055 0.0053 0.0037 0.0054 0.0044 0.0267 0.0504 0.0202 0.0441 0.0086 0.0047 0.0042 0.0061 0.0105 0.0050 0.0717 0.0347