Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0536 0.3953 0.0090 0.0132 0.1339 0.0423 0.1499 0.0196 0.0297 0.0410 0.0146 0.0259 0.0273 0.0675 0.0186
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0584 0.3290 0.0078 0.0097 0.0827 0.0373 0.1179 0.0155 0.0219 0.0373 0.0333 0.0127 0.0194 0.0261 0.0551 0.0176
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0534 0.3846 0.0091 0.0106 0.0971 0.0404 0.1351 0.0212 0.0257 0.0396 0.0316 0.0137 0.0242 0.0261 0.0555 0.0221
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0636 0.3937 0.0084 0.0112 0.0819 0.1316 0.0173 0.0342 0.0396 0.0293 0.0182 0.0169 0.0301 0.0401 0.0284
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0672 0.3425 0.0097 0.0971 0.0389 0.1357 0.0182 0.0249 0.0413 0.0340 0.0148 0.0173 0.0280 0.0630 0.0153
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0565 0.3401 0.0103 0.0105 0.1091 0.0362 0.1271 0.0161 0.0274 0.0410 0.0269 0.0277 0.0242 0.0301 0.0512 0.0319
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0670 0.3745 0.0091 0.0111 0.1156 0.0320 0.1513 0.0162 0.0292 0.0352 0.0291 0.0139 0.0150 0.0296 0.0609 0.0169
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0500 0.3413 0.0110 0.0119 0.1075 0.0342 0.1191 0.0170 0.0301 0.0419 0.0306 0.0266 0.0271 0.0299 0.0746 0.0274
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0440 0.3802 0.0123 0.1377 0.0436 0.1342 0.0264 0.0302 0.0426 0.0341 0.0176 0.0262 0.0289 0.0254 0.0188
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0556 0.2916 0.0084 0.0100 0.0833 0.0340 0.1112 0.0165 0.0232 0.0380 0.0321 0.0122 0.0158 0.0308 0.0577 0.0176
WM-115 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.0569 0.3861 0.0103 0.0106 0.0996 0.0474 0.1471 0.0227 0.0272 0.0404 0.0334 0.0167 0.0221 0.0300 0.0748 0.0208
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0589 0.3690 0.0073 0.0087 0.0878 0.0349 0.1433 0.0142 0.0256 0.0368 0.0130 0.0177 0.0304 0.0629
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0554 0.3460 0.0111 0.0095 0.0956 0.0434 0.1346 0.0168 0.0237 0.0378 0.0336 0.0123 0.0203 0.0289 0.0602 0.0186
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0523 0.3571 0.0114 0.0119 0.1136 0.0335 0.1271 0.0174 0.0276 0.0425 0.0299 0.0220 0.0243 0.0328 0.0701 0.0280
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0629 0.4464 0.0107 0.0110 0.1133 0.0396 0.1198 0.0176 0.0286 0.0340 0.0344 0.0142 0.0134 0.0274 0.0616 0.0198
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0456 0.3509 0.0066 0.1004 0.0320 0.1095 0.0182 0.0267 0.0372 0.0329 0.0138 0.0262 0.0493 0.0156
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0448 0.3937 0.0081 0.0134 0.1314 0.0413 0.1174 0.0217 0.0298 0.0440 0.0371 0.0181 0.0259 0.0289 0.0518 0.0193
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0583 0.3165 0.0068 0.0121 0.0781 0.0349 0.0995 0.0234 0.0229 0.0365 0.0306 0.0152 0.0287 0.0569 0.0192
WM-115 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.0543 0.3817 0.0093 0.0132 0.0965 0.1423 0.0273 0.0422 0.0346 0.0155 0.0205 0.0299 0.0581 0.0219
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0436 0.3378 0.0125 0.0143 0.0639 0.0329 0.1223 0.0168 0.0217 0.0350 0.0260 0.0129 0.0183 0.0249 0.0348
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0619 0.3226 0.0083 0.0109 0.0811 0.0298 0.0927 0.0228 0.0240 0.0388 0.0301 0.0161 0.0147 0.0272 0.0511 0.0146
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0433 0.3077 0.0089 0.0111 0.0934 0.0322 0.1044 0.0164 0.0246 0.0415 0.0311 0.0132 0.0257 0.0257 0.0676 0.0169
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0479 0.3521 0.0126 0.0117 0.1339 0.0373 0.1188 0.0195 0.0295 0.0465 0.0333 0.0161 0.0221 0.0273 0.0564 0.0181
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0549 0.3906 0.0084 0.0122 0.1223 0.0352 0.1401 0.0171 0.0265 0.0449 0.0331 0.0111 0.0239 0.0484 0.0165
WM-115 0.0000
uM
 24
hr
 5
Odyssey/Microvigene
 0.0637 0.4546 0.0086 0.0137 0.1172 0.0434 0.1488 0.0199 0.0282 0.0367 0.0341 0.0147 0.0225 0.0294 0.0614 0.0192