Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0596 0.2967 0.0052 0.0420 0.0305 0.1006 0.0214 0.0252 0.0458 0.0259 0.0344 0.0201 0.0249 0.0538 0.0169
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0602 0.3472 0.0051 0.0069 0.0632 0.0353 0.1046 0.0235 0.0298 0.0480 0.0318 0.0265 0.0179 0.0287 0.0431 0.0191
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0747 0.2933 0.0039 0.0065 0.0423 0.0293 0.0992 0.0195 0.0220 0.0389 0.0273 0.0186 0.0110 0.0283 0.0387 0.0179
WM-115 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.0678 0.3571 0.0060 0.0066 0.0537 0.0413 0.1131 0.0258 0.0251 0.0401 0.0299 0.0221 0.0174 0.0254 0.0418 0.0208
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.2833 0.0048 0.0051 0.0400 0.0341 0.0568 0.0235 0.0185 0.0377 0.0223 0.0169 0.0114 0.0277 0.0480 0.0314
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0664 0.3096 0.0056 0.0056 0.0547 0.0399 0.0633 0.0236 0.0210 0.0352 0.0255 0.0231 0.0079 0.0286 0.0412
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0629 0.2315 0.0043 0.0045 0.0388 0.0377 0.0525 0.0285 0.0183 0.0288 0.0248 0.0164 0.0082 0.0289 0.0409 0.0338
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0589 0.2545 0.0054 0.0050 0.0457 0.0415 0.0610 0.0273 0.0196 0.0290 0.0266 0.0171 0.0098 0.0303 0.0362 0.0305
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0581 0.2890 0.0038 0.0065 0.0356 0.0321 0.0841 0.0180 0.0192 0.0388 0.0285 0.0196 0.0171 0.0221 0.0459 0.0186
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0575 0.3155 0.0055 0.0074 0.0489 0.0404 0.0918 0.0213 0.0215 0.0427 0.0317 0.0219 0.0129 0.0239 0.0461 0.0194
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0578 0.2326 0.0046 0.0052 0.0390 0.0337 0.0608 0.0176 0.0189 0.0284 0.0241 0.0137 0.0070 0.0235 0.0410 0.0316
WM-115 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.0525 0.2632 0.0056 0.0046 0.0486 0.0415 0.0663 0.0235 0.0200 0.0344 0.0284 0.0173 0.0098 0.0277 0.0414 0.0306
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0508 0.3610 0.0147 0.0590 0.0339 0.1028 0.0176 0.0208 0.0361 0.0287 0.0112 0.0150 0.0244 0.0463
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0625 0.3356 0.0066 0.0090 0.0754 0.0333 0.1026 0.0222 0.0381 0.0272 0.0115 0.0113 0.0295 0.0450 0.0159
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0471 0.3096 0.0047 0.0070 0.0711 0.0343 0.0850 0.0158 0.0224 0.0358 0.0278 0.0103 0.0219 0.0276 0.0409 0.0173
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0519 0.3436 0.0070 0.0092 0.1017 0.0333 0.0914 0.0147 0.0274 0.0411 0.0299 0.0124 0.0144 0.0274 0.0501 0.0159
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0520 0.3509 0.0093 0.0109 0.0954 0.0425 0.1488 0.0184 0.0235 0.0425 0.0271 0.0133 0.0227 0.0336 0.0562 0.0210
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0619 0.4149 0.0106 0.0113 0.1038 0.0494 0.1508 0.0207 0.0260 0.0380 0.0338 0.0141 0.0205 0.0277 0.0684 0.0211
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0563 0.3175 0.0084 0.0097 0.0784 0.0428 0.1230 0.0149 0.0194 0.0373 0.0313 0.0126 0.0152 0.0308 0.0636 0.0192
WM-115 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.0619 0.3953 0.0100 0.0099 0.0868 0.0406 0.1290 0.0147 0.0198 0.0412 0.0265 0.0142 0.0207 0.0283 0.0699 0.0208
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0490 0.3802 0.0114 0.0733 0.0376 0.1634 0.0188 0.0336 0.0357 0.0300 0.0147 0.0159 0.0238 0.0429 0.0372
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0607 0.3049 0.0108 0.0096 0.0836 0.0336 0.1112 0.0147 0.0252 0.0378 0.0327 0.0140 0.0168 0.0294 0.0512 0.0140
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0496 0.3030 0.0105 0.1129 0.0348 0.1105 0.0281 0.0387 0.0271 0.0289 0.0689 0.0321
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0514 0.3279 0.0106 0.0125 0.1179 0.0345 0.1188 0.0248 0.0282 0.0359 0.0328 0.0159 0.0191 0.0283 0.0587 0.0130
WM-115 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.0507 0.3460 0.0104 0.0127 0.1126 0.0367 0.1264 0.0182 0.0266 0.0457 0.0335 0.0212 0.0262 0.0577 0.0231