Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0479 0.3021 0.0023 0.0024 0.0045 0.0488 0.0728 0.0163 0.0106 0.1389 0.0073 0.0133 0.0237 0.0039 0.0047 0.0032 0.0153 0.0075 0.0026 0.0218 0.0199
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0471 0.3333 0.0022 0.0023 0.0040 0.0452 0.0541 0.0121 0.0121 0.1227 0.0072 0.0130 0.0241 0.0042 0.0044 0.0028 0.0117 0.0081 0.0026 0.0217 0.0347
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0957 0.3378 0.0034 0.0034 0.0131 0.1284 0.0933 0.0249 0.0251 0.0978 0.0190 0.0321 0.0447 0.0089 0.0061 0.0054 0.0199 0.0276 0.0039 0.0519 0.0255
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.1110 0.3817 0.0035 0.0031 0.0098 0.1063 0.0765 0.0196 0.0243 0.1046 0.0194 0.0320 0.0412 0.0083 0.0060 0.0051 0.0151 0.0214 0.0035 0.0575 0.0296
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0705 0.2967 0.0027 0.0030 0.0053 0.0697 0.0683 0.0211 0.0127 0.1002 0.0095 0.0171 0.0249 0.0048 0.0087 0.0039 0.0124 0.0087 0.0027 0.0358 0.0173
WM-115 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0756 0.3571 0.0031 0.0042 0.0063 0.0716 0.0679 0.0187 0.0098 0.1319 0.0084 0.0157 0.0246 0.0057 0.0084 0.0032 0.0153 0.0116 0.0025 0.0335
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.1330 0.3906 0.0037 0.0038 0.0107 0.0925 0.1214 0.0315 0.0184 0.0992 0.0188 0.0386 0.0318 0.0084 0.0084 0.0042 0.0154 0.0211 0.0038 0.0534 0.0330
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0961 0.4425 0.0039 0.0041 0.0131 0.0875 0.1233 0.0221 0.0195 0.1095 0.0178 0.0344 0.0286 0.0064 0.0088 0.0044 0.0171 0.0230 0.0039 0.0673 0.0189
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0683 0.3333 0.0034 0.0033 0.0056 0.0571 0.0808 0.0262 0.0124 0.0961 0.0102 0.0199 0.0195 0.0038 0.0080 0.0034 0.0154 0.0099 0.0031 0.0390 0.0250
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0704 0.4292 0.0040 0.0033 0.0077 0.0591 0.0981 0.0247 0.0124 0.0996 0.0117 0.0214 0.0229 0.0051 0.0067 0.0037 0.0181 0.0109 0.0029 0.0514 0.0260
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0733 0.3774 0.0033 0.0028 0.0048 0.0362 0.0659 0.0212 0.0113 0.0866 0.0091 0.0149 0.0169 0.0038 0.0066 0.0035 0.0115 0.0070 0.0031 0.0325 0.0234
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0529 0.4464 0.0035 0.0028 0.0049 0.0459 0.0821 0.0177 0.0134 0.0926 0.0083 0.0172 0.0199 0.0038 0.0065 0.0032 0.0131 0.0068 0.0027 0.0458 0.0208
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
WM-115 0.0000
uM
 48
hr
 1
InnoScan/Mapix
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1350 0.3559 0.0049 0.0045 0.0108 0.1177 0.1692 0.0427 0.0295 0.0959 0.0258 0.0424 0.0330 0.0092 0.0158 0.0038 0.0234 0.0305 0.0043 0.0850 0.0256
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1192 0.3861 0.0047 0.0041 0.0109 0.1104 0.1342 0.0379 0.0296 0.1089 0.0256 0.0428 0.0320 0.0082 0.0120 0.0040 0.0233 0.0270 0.0043 0.0863 0.0319
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.1029 0.3195 0.0028 0.0030 0.0082 0.0857 0.0743 0.0192 0.0184 0.0913 0.0160 0.0292 0.0319 0.0069 0.0087 0.0035 0.0142 0.0202 0.0033 0.0682 0.0179
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0990 0.3937 0.0039 0.0029 0.0099 0.0913 0.0903 0.0206 0.0180 0.0994 0.0172 0.0297 0.0314 0.0071 0.0077 0.0044 0.0164 0.0204 0.0035 0.0826 0.0209
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0488 0.3115 0.0024 0.0031 0.0048 0.0482 0.0637 0.0167 0.0095 0.0704 0.0061 0.0152 0.0177 0.0036 0.0077 0.0030 0.0117 0.0083 0.0027 0.0386 0.0194
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0419 0.3663 0.0030 0.0025 0.0056 0.0513 0.0605 0.0171 0.0098 0.0838 0.0061 0.0145 0.0172 0.0035 0.0079 0.0032 0.0103 0.0087 0.0027 0.0417 0.0164
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
WM-115 0.0000
uM
 48
hr
 2
InnoScan/Mapix
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1134 0.3268 0.0032 0.0039 0.0116 0.1046 0.0935 0.0242 0.0259 0.0959 0.0203 0.0381 0.0307 0.0075 0.0094 0.0037 0.0159 0.0207 0.0036 0.0981 0.0228
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.1174 0.3571 0.0040 0.0037 0.0100 0.1105 0.0806 0.0210 0.0228 0.1044 0.0198 0.0350 0.0324 0.0061 0.0091 0.0038 0.0158 0.0171 0.0036 0.0966 0.0189
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0775 0.3021 0.0032 0.0030 0.0072 0.0812 0.0763 0.0218 0.0137 0.0864 0.0101 0.0228 0.0214 0.0039 0.0076 0.0038 0.0151 0.0097 0.0026 0.0643 0.0256