Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0734 0.2890 0.0029 0.0023 0.0079 0.0671 0.0711 0.0134 0.0122 0.0788 0.0159 0.0272 0.0459 0.0073 0.0021 0.0079 0.0209 0.0027 0.0132 0.0149
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0605 0.3448 0.0024 0.0017 0.0089 0.0564 0.0643 0.0127 0.0123 0.0973 0.0135 0.0244 0.0438 0.0055 0.0067 0.0020 0.0095 0.0142 0.0026 0.0137 0.0084
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0410 0.2907 0.0025 0.0018 0.0040 0.0441 0.0434 0.0105 0.0092 0.0760 0.0098 0.0144 0.0295 0.0033 0.0061 0.0023 0.0066 0.0091 0.0024 0.0095 0.0114
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0390 0.3584 0.0026 0.0021 0.0050 0.0416 0.0585 0.0101 0.0090 0.0813 0.0095 0.0127 0.0308 0.0038 0.0060 0.0030 0.0080 0.0107 0.0023 0.0122 0.0103
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0625 0.3003 0.0021 0.0019 0.0061 0.0482 0.0429 0.0108 0.0106 0.0747 0.0123 0.0173 0.0397 0.0055 0.0053 0.0026 0.0065 0.0148 0.0022 0.0095 0.0109
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0530 0.3584 0.0024 0.0020 0.0066 0.0511 0.0455 0.0101 0.0100 0.0943 0.0113 0.0156 0.0389 0.0047 0.0061 0.0032 0.0070 0.0128 0.0117 0.0096
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0459 0.3185 0.0026 0.0023 0.0041 0.0365 0.0537 0.0115 0.0090 0.0909 0.0081 0.0128 0.0348 0.0037 0.0059 0.0022 0.0079 0.0063 0.0027 0.0082 0.0154
WM-115 0.0000
uM
 1
hr
 1
InnoScan/Mapix
 0.0527 0.3610 0.0031 0.0019 0.0065 0.0402 0.0754 0.0099 0.0097 0.0902 0.0082 0.0140 0.0338 0.0043 0.0057 0.0032 0.0102 0.0107 0.0034 0.0104 0.0146
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0782 0.2732 0.0025 0.0017 0.0134 0.0776 0.0694 0.0117 0.0155 0.0810 0.0197 0.0259 0.0519 0.0071 0.0077 0.0025 0.0097 0.0279 0.0028 0.0183 0.0128
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0740 0.3155 0.0029 0.0012 0.0125 0.0742 0.0513 0.0113 0.0144 0.0965 0.0177 0.0241 0.0503 0.0068 0.0070 0.0021 0.0090 0.0191 0.0024 0.0172 0.0107
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0476 0.2857 0.0019 0.0021 0.0046 0.0417 0.0507 0.0103 0.0104 0.0847 0.0093 0.0151 0.0307 0.0037 0.0070 0.0023 0.0078 0.0135 0.0023 0.0099 0.0112
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0452 0.3356 0.0027 0.0017 0.0061 0.0424 0.0493 0.0111 0.0092 0.0850 0.0099 0.0143 0.0298 0.0051 0.0081 0.0024 0.0089 0.0115 0.0021 0.0114 0.0109
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0610 0.2778 0.0020 0.0023 0.0084 0.0611 0.0530 0.0100 0.0125 0.0883 0.0152 0.0204 0.0464 0.0063 0.0059 0.0022 0.0074 0.0180 0.0024 0.0117 0.0100
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0591 0.3125 0.0027 0.0013 0.0089 0.0587 0.0457 0.0090 0.0108 0.0945 0.0146 0.0183 0.0451 0.0062 0.0060 0.0018 0.0083 0.0171 0.0022 0.0139 0.0092
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0458 0.2976 0.0018 0.0018 0.0032 0.0372 0.0395 0.0096 0.0088 0.0883 0.0081 0.0128 0.0315 0.0033 0.0056 0.0022 0.0090 0.0065 0.0025 0.0100 0.0098
WM-115 0.0000
uM
 1
hr
 2
InnoScan/Mapix
 0.0448 0.3584 0.0030 0.0018 0.0056 0.0405 0.0685 0.0094 0.0097 0.1011 0.0081 0.0131 0.0314 0.0045 0.0052 0.0025 0.0095 0.0099 0.0024 0.0117 0.0115
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0627 0.2857 0.0024 0.0021 0.0101 0.0541 0.0587 0.0126 0.0124 0.0702 0.0134 0.0187 0.0397 0.0059 0.0071 0.0024 0.0078 0.0169 0.0024 0.0163 0.0110
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0639 0.3125 0.0024 0.0014 0.0094 0.0501 0.0466 0.0102 0.0112 0.0858 0.0125 0.0177 0.0383 0.0054 0.0080 0.0024 0.0085 0.0148 0.0023 0.0161 0.0088
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0465 0.2809 0.0023 0.0020 0.0108 0.0358 0.0576 0.0106 0.0090 0.0729 0.0093 0.0132 0.0285 0.0039 0.0067 0.0028 0.0100 0.0087 0.0117 0.0143
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0490 0.3068 0.0028 0.0017 0.0109 0.0379 0.0565 0.0090 0.0094 0.0880 0.0097 0.0127 0.0302 0.0044 0.0063 0.0027 0.0118 0.0090 0.0026 0.0115 0.0114
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0650 0.2841 0.0021 0.0025 0.0111 0.0693 0.0588 0.0149 0.0130 0.0903 0.0165 0.0201 0.0517 0.0066 0.0061 0.0038 0.0084 0.0270 0.0025 0.0158 0.0134
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0651 0.3268 0.0024 0.0023 0.0096 0.0656 0.0536 0.0089 0.0138 0.0974 0.0152 0.0187 0.0517 0.0089 0.0064 0.0035 0.0091 0.0210 0.0026 0.0163 0.0089
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0407 0.2865 0.0018 0.0355 0.0422 0.0088 0.0079 0.0679 0.0099 0.0130 0.0287 0.0043 0.0041 0.0029 0.0098 0.0071 0.0024 0.0113 0.0139
WM-115 0.0000
uM
 1
hr
 3
InnoScan/Mapix
 0.0481 0.3300 0.0025 0.0016 0.0086 0.0398 0.0625 0.0078 0.0087 0.0842 0.0094 0.0126 0.0327 0.0047 0.0051 0.0023 0.0087 0.0101 0.0023 0.0104 0.0105
WM-115 0.0000
uM
 1
hr
 4
InnoScan/Mapix
 0.0600 0.2865 0.0025 0.0020 0.0132 0.0590 0.0861 0.0144 0.0129 0.0597 0.0150 0.0217 0.0475 0.0077 0.0067 0.0036 0.0100 0.0200 0.0036 0.0136 0.0202