Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0773 0.3597 0.0037 0.0029 0.0086 0.0672 0.0775 0.0178 0.0148 0.1016 0.0129 0.0221 0.0229 0.0049 0.0077 0.0032 0.0150 0.0127 0.0025 0.0681 0.0229
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0963 0.3401 0.0025 0.0030 0.0124 0.0861 0.0678 0.0201 0.0183 0.0877 0.0133 0.0301 0.0264 0.0058 0.0094 0.0043 0.0137 0.0156 0.0049 0.0778 0.0245
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0949 0.3817 0.0039 0.0026 0.0111 0.0892 0.0669 0.0168 0.0168 0.0991 0.0141 0.0293 0.0265 0.0065 0.0077 0.0054 0.0150 0.0170 0.0031 0.0816 0.0203
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0602 0.2994 0.0026 0.0026 0.0033 0.0400 0.0606 0.0176 0.0096 0.0800 0.0079 0.0145 0.0172 0.0029 0.0060 0.0026 0.0138 0.0060 0.0026 0.0392 0.0189
WM-115 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0782 0.3922 0.0032 0.0032 0.0059 0.0510 0.0684 0.0178 0.0100 0.0974 0.0066 0.0169 0.0224 0.0042 0.0062 0.0031 0.0166 0.0087 0.0025 0.0414
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1129 0.3300 0.0035 0.0038 0.0158 0.1255 0.1261 0.0323 0.0249 0.0870 0.0192 0.0408 0.0335 0.0081 0.0089 0.0055 0.0192 0.0235 0.0040 0.1075 0.0339
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1217 0.3704 0.0037 0.0042 0.0124 0.1152 0.0982 0.0264 0.0242 0.0955 0.0183 0.0334 0.0323 0.0083 0.0091 0.0055 0.0165 0.0198 0.0046 0.1153 0.0258
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1159 0.3401 0.0039 0.0031 0.0116 0.1325 0.1191 0.0292 0.0264 0.0988 0.0168 0.0375 0.0388 0.0073 0.0112 0.0050 0.0259 0.0187 0.0039 0.0818 0.0325
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.1316 0.3953 0.0037 0.0043 0.0122 0.1299 0.1109 0.0259 0.0252 0.1164 0.0169 0.0339 0.0361 0.0092 0.0108 0.0041 0.0208 0.0238 0.0043 0.0763 0.0432
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0851 0.3378 0.0026 0.0031 0.0119 0.0781 0.0875 0.0203 0.0149 0.0750 0.0123 0.0303 0.0240 0.0058 0.0076 0.0044 0.0145 0.0154 0.0027 0.0735 0.0203
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0970 0.3891 0.0030 0.0032 0.0097 0.0740 0.0565 0.0156 0.0134 0.0852 0.0123 0.0244 0.0236 0.0074 0.0077 0.0033 0.0103 0.0155 0.0029 0.0806 0.0156
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0691 0.3268 0.0027 0.0025 0.0041 0.0525 0.0646 0.0162 0.0114 0.0625 0.0073 0.0153 0.0209 0.0035 0.0066 0.0030 0.0130 0.0073 0.0025 0.0466 0.0360
WM-115 0.0000
uM
 48
hr
 4
InnoScan/Mapix
 0.0779 0.3891 0.0032 0.0031 0.0061 0.0560 0.0702 0.0163 0.0111 0.0874 0.0070 0.0161 0.0220 0.0048 0.0063 0.0031 0.0154 0.0114 0.0024 0.0446 0.0542
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1445 0.3831 0.0049 0.0042 0.0126 0.1241 0.1445 0.0331 0.0279 0.1009 0.0300 0.0454 0.0402 0.0110 0.0092 0.0047 0.0167 0.0314 0.0050 0.0970 0.0334
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1261 0.4444 0.0061 0.0054 0.0162 0.1104 0.1661 0.0306 0.0320 0.1045 0.0263 0.0419 0.0367 0.0085 0.0115 0.0049 0.0252 0.0314 0.0051 0.1006 0.0254
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1212 0.3636 0.0035 0.0035 0.0083 0.0869 0.0709 0.0233 0.0155 0.0938 0.0150 0.0325 0.0272 0.0061 0.0084 0.0040 0.0135 0.0176 0.0034 0.0545 0.0310
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0907 0.4348 0.0037 0.0032 0.0098 0.0804 0.0986 0.0215 0.0156 0.1091 0.0174 0.0266 0.0273 0.0065 0.0069 0.0043 0.0138 0.0156 0.0030 0.0815 0.0219
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1479 0.3937 0.0056 0.0060 0.0128 0.1148 0.1653 0.0423 0.0340 0.0997 0.0250 0.0465 0.0386 0.0104 0.0145 0.0051 0.0165 0.0296 0.0050 0.0823
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.1261 0.4608 0.0072 0.0054 0.0127 0.1099 0.1610 0.0379 0.0286 0.1034 0.0224 0.0478 0.0360 0.0098 0.0113 0.0049 0.0215 0.0252 0.0046 0.1174 0.0279
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0824 0.3345 0.0039 0.0038 0.0098 0.0641 0.1031 0.0292 0.0153 0.0873 0.0102 0.0209 0.0272 0.0044 0.0086 0.0037 0.0148 0.0128 0.0031 0.0587 0.0291
WM-115 0.0000
uM
 48
hr
 5
InnoScan/Mapix
 0.0863 0.4115 0.0045 0.0037 0.0130 0.0698 0.1040 0.0283 0.0141 0.0914 0.0104 0.0244 0.0251 0.0052 0.0079 0.0037 0.0176 0.0145 0.0032 0.0752 0.0267
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1130 0.3345 0.0034 0.0036 0.0114 0.1174 0.0294 0.0221 0.0932 0.0204 0.0385 0.0313 0.0089 0.0037 0.0136 0.0257 0.0037 0.0799 0.0174
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.1114 0.3597 0.0038 0.0039 0.0099 0.0988 0.0847 0.0223 0.0235 0.1012 0.0193 0.0382 0.0276 0.0059 0.0083 0.0040 0.0141 0.0203 0.0038 0.0806 0.0144
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0717 0.3030 0.0023 0.0026 0.0066 0.0692 0.0646 0.0209 0.0124 0.0929 0.0103 0.0205 0.0244 0.0040 0.0061 0.0032 0.0117 0.0123 0.0027 0.0453 0.0166
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0700 0.3521 0.0032 0.0028 0.0079 0.0708 0.0627 0.0224 0.0125 0.1013 0.0109 0.0195 0.0226 0.0044 0.0081 0.0031 0.0105 0.0115 0.0027 0.0596 0.0183