Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0765 0.3236 0.0027 0.0030 0.0065 0.0597 0.0612 0.0260 0.0110 0.0839 0.0113 0.0223 0.0240 0.0048 0.0078 0.0030 0.0096 0.0127 0.0026 0.0537 0.0181
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0719 0.3774 0.0035 0.0023 0.0063 0.0583 0.0599 0.0178 0.0114 0.0903 0.0117 0.0240 0.0191 0.0041 0.0066 0.0034 0.0119 0.0106 0.0024 0.0630 0.0126
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0663 0.2976 0.0027 0.0028 0.0034 0.0477 0.0701 0.0214 0.0131 0.0806 0.0078 0.0157 0.0208 0.0033 0.0065 0.0030 0.0129 0.0082 0.0025 0.0413 0.0193
WM-115 0.0000
uM
 48
hr
 6
InnoScan/Mapix
 0.0653 0.3677 0.0035 0.0028 0.0049 0.0535 0.0654 0.0204 0.0131 0.0839 0.0078 0.0169 0.0196 0.0040 0.0062 0.0032 0.0145 0.0084 0.0027 0.0556
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0835 0.3030 0.0024 0.0026 0.0083 0.0628 0.0216 0.0133 0.0836 0.0165 0.0242 0.0196 0.0074 0.0029 0.0102 0.0026 0.0531 0.0159
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0794 0.3356 0.0032 0.0063 0.0571 0.0539 0.0211 0.0120 0.1052 0.0134 0.0260 0.0189 0.0038 0.0071 0.0027 0.0087 0.0100 0.0024 0.0568 0.0158
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0517 0.2916 0.0026 0.0028 0.0060 0.0424 0.0596 0.0215 0.0092 0.0847 0.0084 0.0184 0.0158 0.0031 0.0075 0.0034 0.0117 0.0072 0.0028 0.0351 0.0223
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0541 0.3448 0.0035 0.0027 0.0058 0.0413 0.0562 0.0178 0.0090 0.0939 0.0093 0.0196 0.0197 0.0036 0.0072 0.0028 0.0125 0.0070 0.0026 0.0513 0.0166
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0739 0.2985 0.0025 0.0031 0.0066 0.0608 0.0585 0.0167 0.0101 0.0786 0.0089 0.0178 0.0193 0.0039 0.0072 0.0035 0.0111 0.0091 0.0027 0.0544 0.0186
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0559 0.3497 0.0038 0.0029 0.0055 0.0519 0.0584 0.0222 0.0095 0.0851 0.0089 0.0201 0.0158 0.0031 0.0069 0.0030 0.0141 0.0076 0.0025 0.0570 0.0179
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0737 0.2725 0.0025 0.0024 0.0038 0.0411 0.0513 0.0197 0.0140 0.0609 0.0058 0.0165 0.0143 0.0028 0.0077 0.0025 0.0142 0.0070 0.0025 0.0379 0.0197
WM-115 0.0000
uM
 48
hr
 7
InnoScan/Mapix
 0.0635 0.3413 0.0031 0.0026 0.0048 0.0475 0.0570 0.0211 0.0139 0.0728 0.0068 0.0158 0.0165 0.0034 0.0077 0.0030 0.0177 0.0069 0.0025 0.0467 0.0224
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0749 0.2941 0.0027 0.0030 0.0062 0.0568 0.0773 0.0275 0.0150 0.0704 0.0092 0.0215 0.0216 0.0050 0.0082 0.0028 0.0156 0.0096 0.0029 0.0489 0.0242
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0608 0.3086 0.0027 0.0026 0.0052 0.0573 0.0598 0.0196 0.0766 0.0092 0.0151 0.0038 0.0069 0.0033 0.0158 0.0072 0.0030 0.0494 0.0310
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0641 0.2577 0.0028 0.0027 0.0050 0.0584 0.0712 0.0205 0.0140 0.0787 0.0080 0.0158 0.0228 0.0037 0.0077 0.0037 0.0176 0.0079 0.0026 0.0496 0.0210
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0760 0.3086 0.0025 0.0028 0.0050 0.0543 0.0595 0.0167 0.0126 0.0844 0.0079 0.0155 0.0232 0.0040 0.0076 0.0031 0.0141 0.0089 0.0028 0.0500 0.0321
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0867 0.3021 0.0032 0.0029 0.0067 0.0622 0.0927 0.0255 0.0153 0.0769 0.0098 0.0197 0.0255 0.0043 0.0079 0.0037 0.0176 0.0109 0.0029 0.0534 0.0171
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0850 0.3497 0.0030 0.0030 0.0053 0.0543 0.0675 0.0175 0.0150 0.0803 0.0098 0.0178 0.0232 0.0043 0.0071 0.0035 0.0145 0.0091 0.0028 0.0547 0.0160
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0631 0.2778 0.0025 0.0026 0.0044 0.0500 0.0556 0.0221 0.0143 0.0835 0.0076 0.0171 0.0215 0.0037 0.0120 0.0037 0.0197 0.0065 0.0027 0.0303
WM-115 0.0000
uM
 48
hr
 8
InnoScan/Mapix
 0.0842 0.3378 0.0028 0.0028 0.0053 0.0559 0.0580 0.0244 0.0151 0.0873 0.0076 0.0169 0.0227 0.0045 0.0104 0.0032 0.0195 0.0103 0.0030 0.0580 0.0491
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0470 0.2890 0.0030 0.0085 0.0678 0.0788 0.0125 0.0221 0.0780 0.0256 0.0222 0.0322 0.0424 0.0158
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0519 0.3448 0.0049 0.0097 0.0735 0.0291 0.0973 0.0140 0.0251 0.0610 0.0259 0.0157 0.0109 0.0240 0.0252 0.0131
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0546 0.2907 0.0036 0.0078 0.0628 0.0319 0.0760 0.0137 0.0194 0.0723 0.0241 0.0155 0.0155 0.0274 0.0355 0.0152
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0535 0.3584 0.0071 0.0091 0.0693 0.0336 0.0813 0.0164 0.0244 0.0672 0.0261 0.0158 0.0146 0.0259 0.0280 0.0165
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0433 0.3003 0.0039 0.0081 0.0684 0.0309 0.0747 0.0149 0.0236 0.0805 0.0205 0.0214 0.0224 0.0331 0.0202 0.0133