Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0516 0.3584 0.0056 0.0090 0.0770 0.0343 0.0943 0.0153 0.0233 0.0700 0.0285 0.0148 0.0186 0.0249 0.0249 0.0133
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0584 0.3185 0.0032 0.0094 0.0634 0.0321 0.0909 0.0139 0.0199 0.0571 0.0276 0.0128 0.0141 0.0254 0.0229 0.0153
WM-115 0.0000
uM
 1
hr
 1
Odyssey/Microvigene
 0.0521 0.3610 0.0057 0.0100 0.0701 0.0319 0.0902 0.0148 0.0208 0.0596 0.0241 0.0151 0.0200 0.0235 0.0441
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0468 0.2732 0.0063 0.0128 0.0655 0.0252 0.0810 0.0143 0.0257 0.0796 0.0230 0.0285 0.0205 0.0318 0.0483 0.0252
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0519 0.3155 0.0039 0.0113 0.0808 0.0272 0.0965 0.0247 0.0696 0.0254 0.0265 0.0164 0.0274 0.0348 0.0108
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0569 0.2857 0.0054 0.0090 0.0646 0.0291 0.0847 0.0143 0.0225 0.0654 0.0254 0.0245 0.0159 0.0278 0.0382 0.0163
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0479 0.3356 0.0053 0.0095 0.0739 0.0327 0.0850 0.0175 0.0242 0.0590 0.0266 0.0165 0.0214 0.0239 0.0357 0.0159
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0469 0.2778 0.0037 0.0101 0.0677 0.0264 0.0883 0.0134 0.0264 0.0821 0.0253 0.0292 0.0186 0.0399 0.0028
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0425 0.3125 0.0045 0.0090 0.0813 0.0318 0.0945 0.0169 0.0273 0.0718 0.0287 0.0132 0.0189 0.0259 0.0423 0.0133
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0534 0.2976 0.0036 0.0082 0.0648 0.0292 0.0883 0.0128 0.0227 0.0636 0.0299 0.0221 0.0165 0.0279 0.0288 0.0123
WM-115 0.0000
uM
 1
hr
 2
Odyssey/Microvigene
 0.0496 0.3584 0.0056 0.0083 0.0765 0.0339 0.1011 0.0176 0.0272 0.0645 0.0263 0.0145 0.0206 0.0273 0.0318 0.0242
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0463 0.2857 0.0100 0.0602 0.0276 0.0702 0.0155 0.0217 0.0664 0.0276 0.0260 0.0213 0.0243 0.0417 0.0148
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0456 0.3125 0.0045 0.0095 0.0796 0.0322 0.0858 0.0169 0.0247 0.0716 0.0290 0.0210 0.0178 0.0242 0.0261 0.0131
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0541 0.2809 0.0044 0.0213 0.0584 0.0306 0.0729 0.0149 0.0214 0.0602 0.0269 0.0150 0.0162 0.0259 0.0375 0.0159
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0505 0.3068 0.0049 0.0221 0.0658 0.0371 0.0880 0.0209 0.0224 0.0609 0.0291 0.0186 0.0185 0.0237 0.0290 0.0183
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0413 0.2841 0.0043 0.0085 0.0661 0.0238 0.0903 0.0148 0.0255 0.0742 0.0249 0.0223 0.0185 0.0245 0.0375 0.0148
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0416 0.3268 0.0038 0.0105 0.0863 0.0314 0.0974 0.0180 0.0263 0.0803 0.0296 0.0155 0.0206 0.0278 0.0232 0.0169
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0540 0.2865 0.0043 0.0140 0.0604 0.0254 0.0679 0.0151 0.0233 0.0632 0.0261 0.0113 0.0244 0.0243 0.0145
WM-115 0.0000
uM
 1
hr
 3
Odyssey/Microvigene
 0.0452 0.3300 0.0053 0.0192 0.0768 0.0388 0.0842 0.0198 0.0257 0.0693 0.0299 0.0159 0.0183 0.0266 0.0232 0.0160
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0420 0.2865 0.0028 0.0089 0.0540 0.0206 0.0597 0.0138 0.0238 0.0770 0.0253 0.0130 0.0162 0.0206 0.0346 0.0109
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0419 0.3165 0.0048 0.0108 0.0806 0.0329 0.0729 0.0175 0.0261 0.0810 0.0305 0.0189 0.0134 0.0230 0.0246 0.0170
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0464 0.2959 0.0033 0.0087 0.0609 0.0265 0.0589 0.0224 0.0613 0.0259 0.0145 0.0155 0.0240 0.0277 0.0136
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0375 0.3068 0.0060 0.0102 0.0747 0.0366 0.0883 0.0200 0.0250 0.0721 0.0312 0.0194 0.0201 0.0248 0.0308 0.0142
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0416 0.3030 0.0041 0.0100 0.0606 0.0296 0.0670 0.0202 0.0269 0.0795 0.0293 0.0150 0.0218 0.0244 0.0354 0.0127
WM-115 0.0000
uM
 1
hr
 4
Odyssey/Microvigene
 0.0405 0.3448 0.0049 0.0119 0.0918 0.0360 0.0845 0.0231 0.0276 0.0891 0.0377 0.0174 0.0229 0.0298 0.0204 0.0165