Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0355 0.3448 0.0035 0.0018 0.0047 0.0361 0.0610 0.0167 0.0082 0.0785 0.0093 0.0178 0.0281 0.0041 0.0061 0.0024 0.0104 0.0109 0.0022 0.0124 0.0175
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0359 0.2088 0.0020 0.0015 0.0034 0.0208 0.0504 0.0095 0.0077 0.0812 0.0114 0.0116 0.0243 0.0044 0.0052 0.0024 0.0067 0.0096 0.0065 0.0148
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0347 0.2475 0.0027 0.0014 0.0033 0.0226 0.0397 0.0088 0.0068 0.0892 0.0097 0.0099 0.0271 0.0033 0.0040 0.0021 0.0077 0.0100 0.0019 0.0075 0.0147
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0422 0.0019 0.0019 0.0020 0.0188 0.0489 0.0075 0.0070 0.0068 0.0198 0.0289 0.0032 0.0034 0.0022 0.0064 0.0027 0.0095 0.0148
WM-115 0.0000
uM
 5
hr
 2
InnoScan/Mapix
 0.0333 0.2347 0.0029 0.0018 0.0045 0.0207 0.0560 0.0087 0.0073 0.0909 0.0074 0.0142 0.0284 0.0040 0.0026 0.0025 0.0078 0.0096 0.0023 0.0096
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0415 0.2326 0.0021 0.0023 0.0069 0.0360 0.0561 0.0150 0.0115 0.0413 0.0168 0.0276 0.0046 0.0055 0.0021 0.0088 0.0022 0.0119 0.0208
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0512 0.2519 0.0028 0.0069 0.0296 0.0417 0.0112 0.0099 0.0572 0.0102 0.0164 0.0261 0.0048 0.0055 0.0017 0.0111 0.0152 0.0125 0.0112
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0359 0.2725 0.0022 0.0019 0.0040 0.0245 0.0480 0.0106 0.0109 0.0698 0.0087 0.0151 0.0242 0.0036 0.0057 0.0107 0.0069 0.0022 0.0131 0.0134
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0472 0.3040 0.0026 0.0016 0.0039 0.0243 0.0486 0.0085 0.0078 0.0829 0.0094 0.0133 0.0252 0.0043 0.0056 0.0024 0.0102 0.0100 0.0022 0.0116
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0381 0.2519 0.0022 0.0020 0.0049 0.0227 0.0518 0.0118 0.0090 0.0779 0.0100 0.0132 0.0288 0.0042 0.0052 0.0029 0.0086 0.0116 0.0022 0.0120 0.0195
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0427 0.2874 0.0026 0.0017 0.0043 0.0264 0.0438 0.0092 0.0084 0.0858 0.0091 0.0122 0.0282 0.0042 0.0054 0.0029 0.0077 0.0128 0.0022 0.0116 0.0115
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0368 0.0022 0.0018 0.0024 0.0193 0.0454 0.0083 0.0085 0.0075 0.0142 0.0249 0.0033 0.0023 0.0080 0.0072 0.0022 0.0123 0.0210
WM-115 0.0000
uM
 5
hr
 3
InnoScan/Mapix
 0.0424 0.2667 0.0025 0.0017 0.0043 0.0219 0.0583 0.0068 0.0074 0.0852 0.0076 0.0115 0.0254 0.0040 0.0047 0.0026 0.0096 0.0106 0.0106 0.0239
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0491 0.2564 0.0022 0.0021 0.0102 0.0335 0.1032 0.0120 0.0177 0.0297 0.0067 0.0069 0.0049 0.0114 0.0028 0.0151 0.0165
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0544 0.2976 0.0015 0.0089 0.0279 0.0514 0.0162 0.0110 0.0660 0.0109 0.0153 0.0283 0.0062 0.0059 0.0022 0.0090 0.0127 0.0029 0.0160 0.0114
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0435 0.2732 0.0021 0.0017 0.0049 0.0247 0.0664 0.0103 0.0104 0.0716 0.0086 0.0123 0.0249 0.0051 0.0043 0.0030 0.0103 0.0128 0.0028 0.0158 0.0153
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0477 0.2907 0.0021 0.0019 0.0044 0.0267 0.0503 0.0080 0.0090 0.0746 0.0087 0.0129 0.0277 0.0064 0.0048 0.0025 0.0088 0.0128 0.0026 0.0126 0.0148
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0414 0.2778 0.0022 0.0017 0.0066 0.0251 0.0704 0.0121 0.0105 0.0664 0.0113 0.0145 0.0314 0.0054 0.0056 0.0037 0.0111 0.0124 0.0029 0.0135 0.0176
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0503 0.3086 0.0024 0.0018 0.0054 0.0229 0.0525 0.0085 0.0097 0.0781 0.0110 0.0140 0.0281 0.0062 0.0047 0.0031 0.0090 0.0114 0.0029 0.0128 0.0115
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0370 0.0023 0.0015 0.0043 0.0205 0.0817 0.0084 0.0078 0.0084 0.0118 0.0271 0.0043 0.0059 0.0025 0.0091 0.0026 0.0110 0.0247
WM-115 0.0000
uM
 5
hr
 4
InnoScan/Mapix
 0.0446 0.3012 0.0024 0.0020 0.0050 0.0221 0.0651 0.0085 0.0081 0.0715 0.0075 0.0127 0.0267 0.0050 0.0050 0.0024 0.0108 0.0125 0.0090
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0650 0.2283 0.0032 0.0017 0.0061 0.0472 0.0755 0.0144 0.0119 0.0744 0.0223 0.0478 0.0052 0.0020 0.0109 0.0252 0.0027 0.0166
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0588 0.2801 0.0024 0.0014 0.0039 0.0427 0.0707 0.0118 0.0152 0.0235 0.0368 0.0051 0.0056 0.0022 0.0126 0.0230 0.0027 0.0166 0.0089
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0489 0.2571 0.0025 0.0015 0.0031 0.0269 0.0553 0.0133 0.0084 0.0815 0.0102 0.0170 0.0250 0.0036 0.0056 0.0030 0.0079 0.0106 0.0024 0.0106 0.0169
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0466 0.2985 0.0028 0.0020 0.0039 0.0261 0.0657 0.0106 0.0089 0.0792 0.0110 0.0133 0.0267 0.0045 0.0051 0.0032 0.0092 0.0156 0.0020 0.0152 0.0112