Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0673 0.2632 0.0026 0.0023 0.0049 0.0293 0.0589 0.0139 0.0116 0.0771 0.0165 0.0185 0.0351 0.0058 0.0058 0.0027 0.0080 0.0176 0.0029 0.0175 0.0145
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0532 0.3155 0.0030 0.0014 0.0054 0.0270 0.0521 0.0102 0.0105 0.0803 0.0104 0.0207 0.0311 0.0043 0.0054 0.0022 0.0090 0.0135 0.0035 0.0185 0.0102
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0370 0.2506 0.0027 0.0016 0.0027 0.0199 0.0569 0.0109 0.0085 0.0760 0.0082 0.0135 0.0269 0.0032 0.0049 0.0025 0.0081 0.0087 0.0026 0.0104 0.0148
WM-115 0.0000
uM
 5
hr
 5
InnoScan/Mapix
 0.0386 0.3049 0.0028 0.0017 0.0043 0.0224 0.0618 0.0101 0.0095 0.0726 0.0085 0.0135 0.0265 0.0047 0.0048 0.0026 0.0101 0.0116 0.0022 0.0134 0.0164
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0651 0.2463 0.0026 0.0014 0.0075 0.0424 0.0614 0.0168 0.0147 0.0757 0.0191 0.0185 0.0298 0.0057 0.0023 0.0091 0.0026 0.0152 0.0124
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0606 0.2950 0.0032 0.0014 0.0058 0.0378 0.0574 0.0154 0.0126 0.0778 0.0138 0.0190 0.0319 0.0042 0.0062 0.0018 0.0086 0.0174 0.0150 0.0115
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0513 0.2347 0.0021 0.0016 0.0034 0.0348 0.0491 0.0127 0.0086 0.0838 0.0094 0.0175 0.0215 0.0038 0.0059 0.0020 0.0090 0.0099 0.0021 0.0112
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0439 0.2660 0.0027 0.0015 0.0042 0.0262 0.0502 0.0107 0.0083 0.0924 0.0098 0.0129 0.0232 0.0037 0.0045 0.0028 0.0091 0.0095 0.0030 0.0145 0.0134
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0420 0.2268 0.0020 0.0045 0.0240 0.0484 0.0113 0.0084 0.0817 0.0135 0.0175 0.0284 0.0043 0.0058 0.0023 0.0079 0.0117 0.0023 0.0136 0.0119
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0400 0.2625 0.0030 0.0013 0.0041 0.0235 0.0479 0.0116 0.0099 0.0915 0.0121 0.0136 0.0299 0.0036 0.0050 0.0023 0.0102 0.0096 0.0022 0.0138
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0409 0.2398 0.0020 0.0017 0.0024 0.0190 0.0571 0.0090 0.0079 0.0754 0.0088 0.0110 0.0241 0.0029 0.0046 0.0022 0.0076 0.0061 0.0023 0.0120 0.0115
WM-115 0.0000
uM
 5
hr
 6
InnoScan/Mapix
 0.0349 0.2793 0.0027 0.0017 0.0036 0.0202 0.0494 0.0094 0.0071 0.0854 0.0074 0.0111 0.0242 0.0035 0.0045 0.0026 0.0087 0.0076 0.0022 0.0133 0.0118
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0426 0.2433 0.0022 0.0013 0.0610 0.0132 0.0103 0.0418 0.0099 0.0162 0.0281 0.0060 0.0023 0.0089 0.0151 0.0024 0.0136 0.0139
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0441 0.2710 0.0023 0.0011 0.0046 0.0266 0.0403 0.0091 0.0097 0.0605 0.0090 0.0168 0.0242 0.0039 0.0045 0.0017 0.0098 0.0089 0.0023 0.0137
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0367 0.2494 0.0021 0.0019 0.0040 0.0280 0.0477 0.0108 0.0092 0.0651 0.0112 0.0118 0.0229 0.0042 0.0053 0.0022 0.0095 0.0108 0.0023 0.0149 0.0150
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0369 0.2801 0.0024 0.0020 0.0037 0.0294 0.0432 0.0088 0.0076 0.0703 0.0088 0.0121 0.0255 0.0044 0.0049 0.0020 0.0103 0.0093 0.0024 0.0132 0.0150
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0519 0.2268 0.0022 0.0023 0.0051 0.0238 0.0601 0.0111 0.0103 0.0786 0.0091 0.0159 0.0282 0.0047 0.0049 0.0032 0.0078 0.0184 0.0021 0.0148 0.0140
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0429 0.2513 0.0028 0.0014 0.0041 0.0249 0.0421 0.0090 0.0088 0.0824 0.0088 0.0134 0.0245 0.0044 0.0043 0.0029 0.0080 0.0094 0.0023 0.0144
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0369 0.2188 0.0025 0.0020 0.0025 0.0205 0.0528 0.0089 0.0084 0.0665 0.0072 0.0105 0.0264 0.0033 0.0043 0.0023 0.0092 0.0078 0.0023 0.0143 0.0169
WM-115 0.0000
uM
 5
hr
 7
InnoScan/Mapix
 0.0396 0.2625 0.0026 0.0017 0.0036 0.0214 0.0512 0.0078 0.0076 0.0800 0.0074 0.0122 0.0284 0.0039 0.0039 0.0025 0.0088 0.0085 0.0022 0.0143 0.0157
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0508 0.2674 0.0025 0.0021 0.0779 0.0139 0.0110 0.0463 0.0103 0.0144 0.0268 0.0042 0.0055 0.0030 0.0091 0.0026 0.0194 0.0209
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0509 0.2857 0.0030 0.0018 0.0045 0.0246 0.0608 0.0101 0.0076 0.0507 0.0105 0.0138 0.0051 0.0025 0.0104 0.0088 0.0024 0.0201 0.0176
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0444 0.2416 0.0023 0.0022 0.0042 0.0221 0.0646 0.0090 0.0093 0.0700 0.0057 0.0100 0.0255 0.0042 0.0049 0.0030 0.0110 0.0070 0.0027 0.0142 0.0158
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0421 0.2646 0.0023 0.0021 0.0037 0.0209 0.0498 0.0075 0.0086 0.0868 0.0055 0.0089 0.0252 0.0042 0.0044 0.0026 0.0083 0.0063 0.0027 0.0134 0.0252
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0432 0.2439 0.0023 0.0018 0.0041 0.0209 0.0619 0.0119 0.0102 0.0538 0.0094 0.0123 0.0280 0.0044 0.0044 0.0033 0.0127 0.0099 0.0026 0.0162 0.0135