Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0455 0.2740 0.0027 0.0017 0.0193 0.0452 0.0081 0.0092 0.0642 0.0084 0.0109 0.0279 0.0046 0.0046 0.0024 0.0084 0.0094 0.0025 0.0164 0.0101
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0471 0.2304 0.0030 0.0026 0.0042 0.0202 0.0097 0.0087 0.0572 0.0091 0.0194 0.0271 0.0045 0.0054 0.0036 0.0123 0.0076 0.0033 0.0176 0.0178
WM-115 0.0000
uM
 5
hr
 8
InnoScan/Mapix
 0.0475 0.2653 0.0032 0.0027 0.0041 0.0217 0.0711 0.0091 0.0100 0.0804 0.0078 0.0145 0.0266 0.0049 0.0052 0.0034 0.0109 0.0086 0.0030 0.0160 0.0326
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0705 0.3205 0.0030 0.0021 0.0040 0.0344 0.0593 0.0147 0.0110 0.0786 0.0177 0.0246 0.0044 0.0084 0.0027 0.0083 0.0029 0.0130 0.0232
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0538 0.3876 0.0032 0.0022 0.0049 0.0310 0.0606 0.0118 0.0118 0.0818 0.0135 0.0136 0.0205 0.0037 0.0083 0.0026 0.0101 0.0100 0.0029 0.0151 0.0201
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0583 0.3311 0.0028 0.0017 0.0030 0.0271 0.0498 0.0132 0.0106 0.0689 0.0131 0.0141 0.0213 0.0037 0.0076 0.0029 0.0080 0.0086 0.0025 0.0127 0.0167
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0540 0.3745 0.0027 0.0021 0.0041 0.0276 0.0596 0.0114 0.0106 0.0728 0.0136 0.0126 0.0229 0.0040 0.0067 0.0033 0.0097 0.0101 0.0022 0.0170 0.0215
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0649 0.3704 0.0029 0.0018 0.0034 0.0243 0.0470 0.0120 0.0094 0.0750 0.0105 0.0135 0.0254 0.0043 0.0067 0.0031 0.0084 0.0098 0.0025 0.0103 0.0212
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0526 0.4405 0.0031 0.0017 0.0037 0.0248 0.0550 0.0114 0.0107 0.0816 0.0107 0.0124 0.0249 0.0037 0.0063 0.0030 0.0094 0.0083 0.0023 0.0135 0.0204
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0549 0.3448 0.0026 0.0017 0.0033 0.0243 0.0560 0.0141 0.0114 0.1005 0.0105 0.0133 0.0216 0.0036 0.0083 0.0026 0.0104 0.0074 0.0026 0.0120 0.0231
WM-115 0.0000
uM
 10
hr
 1
InnoScan/Mapix
 0.0636 0.3861 0.0029 0.0020 0.0047 0.0295 0.0131 0.0123 0.0963 0.0103 0.0134 0.0257 0.0038 0.0084 0.0030 0.0097 0.0028 0.0145
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0745 0.3175 0.0022 0.0016 0.0058 0.0390 0.0133 0.0122 0.0876 0.0155 0.0212 0.0250 0.0056 0.0088 0.0020 0.0113 0.0164 0.0024 0.0171 0.0133
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.3484 0.0029 0.0015 0.0054 0.0330 0.0464 0.0142 0.0112 0.1029 0.0150 0.0195 0.0272 0.0044 0.0083 0.0022 0.0101 0.0125 0.0022 0.0184
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0490 0.3135 0.0021 0.0018 0.0032 0.0290 0.0460 0.0117 0.0086 0.0846 0.0106 0.0134 0.0191 0.0041 0.0074 0.0022 0.0089 0.0096 0.0117 0.0151
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0504 0.3731 0.0033 0.0016 0.0044 0.0293 0.0471 0.0127 0.0090 0.0940 0.0108 0.0134 0.0209 0.0039 0.0066 0.0023 0.0104 0.0089 0.0023 0.0134 0.0192
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0545 0.3145 0.0021 0.0017 0.0039 0.0250 0.0503 0.0120 0.0154 0.1223 0.0107 0.0149 0.0236 0.0048 0.0078 0.0023 0.0082 0.0125 0.0022 0.0155 0.0118
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0502 0.3546 0.0029 0.0016 0.0046 0.0271 0.0465 0.0110 0.0102 0.1389 0.0120 0.0132 0.0231 0.0058 0.0082 0.0029 0.0101 0.0125 0.0022 0.0169 0.0116
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0589 0.3125 0.0021 0.0016 0.0026 0.0206 0.0409 0.0107 0.0093 0.1136 0.0087 0.0120 0.0206 0.0031 0.0061 0.0024 0.0084 0.0062 0.0023 0.0138 0.0139
WM-115 0.0000
uM
 10
hr
 2
InnoScan/Mapix
 0.0598 0.3891 0.0031 0.0018 0.0050 0.0247 0.0518 0.0111 0.0104 0.1193 0.0087 0.0129 0.0225 0.0042 0.0055 0.0027 0.0106 0.0105 0.0023 0.0148 0.0185
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0698 0.3165 0.0023 0.0026 0.0070 0.0406 0.0628 0.0147 0.0148 0.0186 0.0215 0.0267 0.0050 0.0093 0.0029 0.0093 0.0180 0.0024 0.0285 0.0135
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0731 0.3484 0.0025 0.0015 0.0066 0.0424 0.0474 0.0108 0.0155 0.1015 0.0155 0.0204 0.0276 0.0047 0.0087 0.0023 0.0087 0.0136 0.0023 0.0245 0.0113
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0548 0.3040 0.0023 0.0021 0.0033 0.0267 0.0459 0.0115 0.0099 0.1068 0.0101 0.0110 0.0176 0.0037 0.0076 0.0026 0.0099 0.0080 0.0027 0.0150 0.0201
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0504 0.3448 0.0027 0.0018 0.0037 0.0274 0.0433 0.0095 0.0089 0.1186 0.0107 0.0125 0.0223 0.0045 0.0074 0.0022 0.0092 0.0090 0.0025 0.0166 0.0215
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0542 0.2849 0.0022 0.0025 0.0064 0.0282 0.0473 0.0148 0.0103 0.1085 0.0123 0.0217 0.0275 0.0050 0.0099 0.0020 0.0106 0.0125 0.0023 0.0188 0.0223
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0549 0.3322 0.0026 0.0016 0.0052 0.0259 0.0402 0.0113 0.0098 0.1196 0.0120 0.0207 0.0289 0.0053 0.0093 0.0021 0.0122 0.0021 0.0192 0.0104