Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0494 0.0023 0.0017 0.0025 0.0235 0.0418 0.0102 0.0106 0.0087 0.0120 0.0221 0.0035 0.0075 0.0024 0.0092 0.0065 0.0022 0.0152 0.0260
WM-115 0.0000
uM
 10
hr
 3
InnoScan/Mapix
 0.0528 0.3774 0.0029 0.0019 0.0049 0.0254 0.0557 0.0102 0.0091 0.1082 0.0089 0.0131 0.0230 0.0042 0.0075 0.0028 0.0116 0.0114 0.0021 0.0140 0.0299
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0616 0.3290 0.0024 0.0021 0.0084 0.0367 0.0709 0.0169 0.0118 0.0887 0.0145 0.0192 0.0262 0.0059 0.0092 0.0034 0.0116 0.0024 0.0172 0.0268
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0737 0.3690 0.0017 0.0067 0.0438 0.0495 0.0135 0.0108 0.1020 0.0151 0.0165 0.0303 0.0059 0.0083 0.0023 0.0089 0.0113 0.0027 0.0193 0.0295
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0531 0.0021 0.0017 0.0037 0.0279 0.0644 0.0104 0.0097 0.0085 0.0118 0.0213 0.0042 0.0059 0.0032 0.0114 0.0086 0.0026 0.0171 0.0216
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0593 0.3759 0.0021 0.0020 0.0040 0.0268 0.0449 0.0085 0.0093 0.0992 0.0086 0.0121 0.0235 0.0052 0.0057 0.0027 0.0088 0.0101 0.0025 0.0151 0.0343
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0592 0.3322 0.0023 0.0020 0.0063 0.0290 0.0598 0.0142 0.0126 0.0905 0.0113 0.0174 0.0289 0.0046 0.0086 0.0032 0.0125 0.0113 0.0026 0.0182 0.0211
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0687 0.3802 0.0023 0.0020 0.0046 0.0261 0.0442 0.0087 0.0115 0.0907 0.0109 0.0141 0.0231 0.0046 0.0075 0.0025 0.0104 0.0124 0.0026 0.0150 0.0171
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
WM-115 0.0000
uM
 10
hr
 4
InnoScan/Mapix
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0929 0.3268 0.0033 0.0018 0.0073 0.0643 0.0134 0.0974 0.0300 0.0386 0.0082 0.0026 0.0090 0.0038 0.0244 0.0204
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0846 0.4049 0.0030 0.0099 0.0442 0.0682 0.0139 0.0176 0.1033 0.0190 0.0266 0.0340 0.0051 0.0024 0.0108 0.0212 0.0033 0.0241 0.0126
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0597 0.3521 0.0025 0.0018 0.0037 0.0271 0.0517 0.0132 0.0104 0.1006 0.0103 0.0158 0.0211 0.0049 0.0081 0.0033 0.0091 0.0094 0.0025 0.0120 0.0179
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0510 0.3802 0.0029 0.0018 0.0046 0.0265 0.0607 0.0127 0.0105 0.0101 0.0138 0.0244 0.0041 0.0077 0.0031 0.0103 0.0115 0.0022 0.0165 0.0149
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0986 0.3534 0.0029 0.0018 0.0055 0.0314 0.0583 0.0132 0.0108 0.1026 0.0141 0.0204 0.0267 0.0054 0.0064 0.0027 0.0076 0.0161 0.0033 0.0150 0.0150
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0702 0.4184 0.0032 0.0018 0.0061 0.0317 0.0599 0.0144 0.1011 0.0130 0.0222 0.0271 0.0044 0.0086 0.0024 0.0105 0.0103 0.0023 0.0202 0.0096
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0565 0.3356 0.0026 0.0019 0.0037 0.0235 0.0552 0.0132 0.0107 0.0991 0.0104 0.0147 0.0241 0.0039 0.0074 0.0031 0.0093 0.0093 0.0025 0.0163 0.0210
WM-115 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0568 0.3802 0.0030 0.0019 0.0050 0.0262 0.0654 0.0135 0.0103 0.1023 0.0102 0.0149 0.0244 0.0043 0.0072 0.0026 0.0115 0.0107 0.0023 0.0209 0.0306
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0776 0.3155 0.0024 0.0017 0.0072 0.0456 0.0599 0.0929 0.0176 0.0250 0.0281 0.0079 0.0020 0.0113 0.0200 0.0024 0.0259 0.0132
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0873 0.3571 0.0030 0.0014 0.0057 0.0419 0.0467 0.0113 0.0134 0.1063 0.0163 0.0225 0.0270 0.0046 0.0084 0.0020 0.0089 0.0171 0.0022 0.0202 0.0100
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0511 0.3086 0.0022 0.0018 0.0029 0.0269 0.0525 0.0123 0.0088 0.0968 0.0094 0.0125 0.0221 0.0041 0.0076 0.0024 0.0101 0.0069 0.0026 0.0140 0.0137
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0478 0.3677 0.0030 0.0016 0.0038 0.0281 0.0521 0.0108 0.0097 0.1155 0.0095 0.0119 0.0194 0.0035 0.0070 0.0025 0.0116 0.0075 0.0026 0.0173 0.0116
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0534 0.3215 0.0024 0.0019 0.0045 0.0264 0.0433 0.0110 0.0087 0.0929 0.0112 0.0156 0.0240 0.0045 0.0084 0.0025 0.0083 0.0153 0.0022 0.0133 0.0142
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0705 0.3534 0.0027 0.0016 0.0039 0.0255 0.0404 0.0114 0.0095 0.1086 0.0118 0.0142 0.0216 0.0035 0.0079 0.0024 0.0097 0.0107 0.0022 0.0146 0.0129
WM-115 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0539 0.3155 0.0024 0.0021 0.0035 0.0220 0.0475 0.0108 0.0094 0.0951 0.0083 0.0227 0.0201 0.0031 0.0068 0.0025 0.0098 0.0062 0.0024 0.0129 0.0143