Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0815 0.3311 0.0043 0.0068 0.0531 0.0363 0.0689 0.0213 0.0211 0.0461 0.0250 0.0190 0.0114 0.0298 0.0465 0.0225
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0830 0.3745 0.0057 0.0075 0.0586 0.0395 0.0728 0.0270 0.0229 0.0414 0.0260 0.0193 0.0130 0.0279 0.0361 0.0231
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0670 0.3704 0.0040 0.0078 0.0514 0.0381 0.0750 0.0187 0.0232 0.0516 0.0217 0.0209 0.0138 0.0344 0.0312 0.0264
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0830 0.4405 0.0063 0.0073 0.0567 0.0432 0.0816 0.0234 0.0272 0.0442 0.0245 0.0222 0.0118 0.0280 0.0362 0.0253
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0732 0.3448 0.0045 0.0069 0.0456 0.0447 0.1005 0.0212 0.0206 0.0347 0.0278 0.0188 0.0110 0.0275 0.0308 0.0260
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0729 0.3861 0.0067 0.0080 0.0532 0.0368 0.0963 0.0231 0.0224 0.0393 0.0249 0.0218 0.0159 0.0240 0.0508 0.0221
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0664 0.3175 0.0042 0.0084 0.0472 0.0332 0.0876 0.0218 0.0262 0.0529 0.0240 0.0374 0.0226 0.0317 0.0522 0.0272
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0684 0.3484 0.0067 0.0076 0.0604 0.0344 0.1029 0.0190 0.0253 0.0464 0.0270 0.0236 0.0129 0.0282 0.0459 0.0133
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0746 0.3135 0.0041 0.0070 0.0561 0.0317 0.0846 0.0230 0.0429 0.0247 0.0233 0.0146 0.0247 0.0347 0.0204
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0616 0.3731 0.0062 0.0083 0.0594 0.0388 0.0940 0.0224 0.0248 0.0394 0.0302 0.0237 0.0203 0.0252 0.0451 0.0199
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0614 0.3145 0.0048 0.0067 0.0458 0.0329 0.1223 0.0154 0.0250 0.0558 0.0216 0.0342 0.0187 0.0318 0.0404 0.0265
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0635 0.3546 0.0057 0.0069 0.0553 0.0330 0.1389 0.0187 0.0517 0.0285 0.0268 0.0190 0.0272 0.0504 0.0167
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0810 0.3125 0.0044 0.0062 0.0446 0.0353 0.1136 0.0172 0.0232 0.0409 0.0271 0.0212 0.0115 0.0254 0.0393 0.0198
WM-115 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.0684 0.3891 0.0057 0.0069 0.0515 0.0365 0.1193 0.0221 0.0243 0.0428 0.0233 0.0233 0.0184 0.0285 0.0400 0.0288
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0603 0.3165 0.0075 0.0484 0.0234 0.0186 0.0244 0.0457 0.0271 0.0389 0.0190 0.0272 0.0578 0.0262
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0612 0.3484 0.0041 0.0061 0.0629 0.0342 0.1015 0.0248 0.0263 0.0443 0.0295 0.0265 0.0158 0.0239 0.0423 0.0175
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0724 0.3040 0.0046 0.0070 0.0541 0.0303 0.1068 0.0187 0.0239 0.0394 0.0291 0.0283 0.0129 0.0245 0.0539 0.0228
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0621 0.3448 0.0054 0.0080 0.0602 0.0393 0.1186 0.0240 0.0262 0.0406 0.0311 0.0241 0.0179 0.0236 0.0403 0.0242
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0571 0.2849 0.0043 0.0065 0.0424 0.0301 0.1085 0.0168 0.0282 0.0487 0.0334 0.0190 0.0255 0.0529 0.0247
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0518 0.3322 0.0040 0.0092 0.0554 0.0355 0.1196 0.0211 0.0274 0.0528 0.0300 0.0271 0.0224 0.0271 0.0415 0.0160
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
WM-115 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.0681 0.3774 0.0062 0.0081 0.0615 0.0448 0.1082 0.0230 0.0257 0.0450 0.0285 0.0265 0.0165 0.0295 0.0580 0.0217
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0579 0.3290 0.0044 0.0101 0.0442 0.0298 0.0887 0.0192 0.0231 0.0449 0.0150 0.0231 0.0297 0.0191
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene
 0.0582 0.3690 0.0061 0.0068 0.0599 0.0375 0.1020 0.0199 0.0280 0.0518 0.0303 0.0281 0.0141 0.0241 0.0372 0.0230
WM-115 0.0000
uM
 10
hr
 4
Odyssey/Microvigene