Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0174 0.3802 0.0023 0.0031 0.0137 0.1220 0.0840 0.0148 0.0121 0.1634 0.0206 0.0254 0.0735 0.0092 0.0053 0.0033 0.0101 0.0268 0.0036 0.0154 0.0207
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0143 0.3049 0.0026 0.0029 0.0137 0.1054 0.1033 0.0103 0.0140 0.1112 0.0173 0.0213 0.0699 0.0075 0.0043 0.0031 0.0133 0.0264 0.0045 0.0136 0.0207
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0099 0.3030 0.0022 0.0020 0.0125 0.0578 0.0554 0.0104 0.0099 0.1105 0.0096 0.0167 0.0604 0.0067 0.0047 0.0025 0.0088 0.0150 0.0025 0.0060 0.0206
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0091 0.3279 0.0024 0.0020 0.0149 0.0655 0.0523 0.0091 0.0108 0.1188 0.0100 0.0121 0.0524 0.0077 0.0049 0.0029 0.0087 0.0175 0.0031 0.0064 0.0294
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1003 0.3460 0.0034 0.0039 0.0146 0.1736 0.0904 0.0256 0.0280 0.1264 0.0249 0.0452 0.0464 0.0105 0.0083 0.0042 0.0132 0.0319 0.0043 0.0539 0.0174
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1057 0.3953 0.0040 0.0047 0.0158 0.1626 0.1049 0.0246 0.0287 0.1499 0.0258 0.0398 0.0502 0.0092 0.0089 0.0149 0.0259 0.0040 0.0635 0.0246
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0608 0.3290 0.0028 0.0025 0.0588 0.0674 0.0171 0.0127 0.1179 0.0108 0.0189 0.0281 0.0042 0.0057 0.0028 0.0114 0.0100 0.0029 0.0255 0.0169
WM-115 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0688 0.3846 0.0042 0.0032 0.0096 0.0710 0.0774 0.0184 0.0151 0.1351 0.0103 0.0192 0.0290 0.0053 0.0060 0.0033 0.0139 0.0144 0.0029 0.0287 0.0265
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0285 0.3937 0.0022 0.0030 0.0175 0.1754 0.0873 0.0119 0.0155 0.1316 0.0187 0.0245 0.0502 0.0102 0.0051 0.0029 0.0086 0.0236 0.0034 0.0218 0.0218
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0253 0.3425 0.0025 0.0017 0.0163 0.1267 0.0557 0.0164 0.0155 0.1357 0.0172 0.0234 0.0493 0.0083 0.0048 0.0032 0.0101 0.0175 0.0034 0.0197 0.0209
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0155 0.3401 0.0017 0.0025 0.0068 0.0629 0.0495 0.0102 0.0078 0.1271 0.0093 0.0145 0.0355 0.0069 0.0046 0.0034 0.0083 0.0119 0.0025 0.0127 0.0258
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0152 0.3745 0.0022 0.0017 0.0069 0.0610 0.0525 0.0078 0.0105 0.1513 0.0091 0.0148 0.0352 0.0062 0.0045 0.0023 0.0070 0.0124 0.0024 0.0128 0.0173
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1019 0.3413 0.0033 0.0050 0.0177 0.1883 0.1008 0.0344 0.0304 0.1191 0.0242 0.0421 0.0455 0.0096 0.0077 0.0059 0.0146 0.0370 0.0042 0.0637 0.0242
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1041 0.3802 0.0045 0.0048 0.0162 0.1639 0.1264 0.0296 0.0298 0.1342 0.0243 0.0419 0.0463 0.0117 0.0079 0.0067 0.0165 0.0301 0.0042 0.0665 0.0181
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0680 0.2916 0.0025 0.0026 0.0041 0.0700 0.0655 0.0180 0.0157 0.1112 0.0100 0.0172 0.0279 0.0033 0.0053 0.0029 0.0108 0.0083 0.0030 0.0368 0.0236
WM-115 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0691 0.3861 0.0038 0.0032 0.0098 0.0772 0.0801 0.0171 0.0145 0.1471 0.0103 0.0200 0.0240 0.0058 0.0061 0.0038 0.0147 0.0150 0.0029 0.0357 0.0230
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0406 0.3690 0.0022 0.0032 0.0188 0.1271 0.0861 0.0189 0.0170 0.1433 0.0175 0.0237 0.0371 0.0084 0.0059 0.0044 0.0172 0.0224 0.0031 0.0382 0.0185
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0441 0.3460 0.0028 0.0025 0.0151 0.1142 0.0645 0.0138 0.0160 0.1346 0.0150 0.0206 0.0326 0.0077 0.0047 0.0045 0.0105 0.0164 0.0038 0.0417 0.0178
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0350 0.3571 0.0022 0.0023 0.0075 0.0776 0.0667 0.0147 0.0160 0.1271 0.0112 0.0183 0.0303 0.0062 0.0046 0.0035 0.0130 0.0128 0.0031 0.0326 0.0206
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0402 0.4464 0.0025 0.0029 0.0085 0.0737 0.0562 0.0127 0.0145 0.1198 0.0114 0.0165 0.0346 0.0076 0.0046 0.0030 0.0089 0.0166 0.0032 0.0325 0.0247
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1020 0.3509 0.0036 0.0035 0.0189 0.1416 0.1264 0.0212 0.1095 0.0207 0.0342 0.0419 0.0091 0.0085 0.0047 0.0218 0.0039 0.0478 0.0266
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.1171 0.3937 0.0035 0.0037 0.0157 0.1269 0.0988 0.0175 0.0182 0.1174 0.0204 0.0347 0.0392 0.0095 0.0082 0.0054 0.0169 0.0243 0.0041 0.0508 0.0156
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0569 0.3165 0.0029 0.0027 0.0078 0.0566 0.0842 0.0199 0.0144 0.0995 0.0092 0.0158 0.0240 0.0045 0.0055 0.0029 0.0136 0.0076 0.0043 0.0309 0.0161
WM-115 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0665 0.3817 0.0032 0.0033 0.0093 0.0589 0.0695 0.0178 0.0140 0.1423 0.0096 0.0156 0.0265 0.0051 0.0066 0.0033 0.0150 0.0114 0.0027 0.0303
WM-115 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0984 0.3378 0.0036 0.0033 0.0172 0.1869 0.1689 0.0301 0.0287 0.1223 0.0327 0.0472 0.0526 0.0134 0.0056 0.0047 0.0138 0.0433 0.0549 0.0308