Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0493 0.2950 0.0081 0.0581 0.0289 0.0778 0.0176 0.0195 0.0500 0.0253 0.0139 0.0096 0.0298 0.0398 0.0094
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0479 0.2347 0.0059 0.0081 0.0454 0.0266 0.0838 0.0137 0.0222 0.0504 0.0271 0.0269 0.0192 0.0280 0.0345 0.0201
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0416 0.2660 0.0057 0.0073 0.0583 0.0287 0.0924 0.0179 0.0237 0.0463 0.0277 0.0153 0.0218 0.0248 0.0402 0.0130
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0454 0.2268 0.0052 0.0071 0.0363 0.0198 0.0817 0.0149 0.0222 0.0620 0.0249 0.0251 0.0216 0.0308 0.0483 0.0246
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0473 0.2625 0.0052 0.0067 0.0421 0.0296 0.0915 0.0153 0.0220 0.0564 0.0267 0.0236 0.0174 0.0264 0.0424 0.0138
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0495 0.2398 0.0046 0.0059 0.0371 0.0237 0.0754 0.0140 0.0196 0.0465 0.0233 0.0173 0.0123 0.0269 0.0314 0.0158
WM-115 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.0441 0.2793 0.0047 0.0062 0.0424 0.0312 0.0854 0.0169 0.0228 0.0458 0.0244 0.0131 0.0184 0.0226 0.0365 0.0162
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0355 0.2433 0.0062 0.0446 0.0187 0.0418 0.0161 0.0192 0.0520 0.0205 0.0146 0.0238 0.0391
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0386 0.2710 0.0066 0.0519 0.0282 0.0605 0.0205 0.0233 0.0595 0.0243 0.0158 0.0119 0.0252 0.0234 0.0130
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0426 0.2494 0.0045 0.0069 0.0456 0.0225 0.0651 0.0127 0.0214 0.0468 0.0235 0.0231 0.0168 0.0234 0.0382 0.0133
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0362 0.2801 0.0047 0.0079 0.0593 0.0337 0.0703 0.0177 0.0228 0.0494 0.0240 0.0166 0.0179 0.0232 0.0274 0.0143
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0373 0.2268 0.0072 0.0377 0.0217 0.0786 0.0156 0.0229 0.0606 0.0142 0.0196 0.0232 0.0414 0.0140
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0375 0.2513 0.0047 0.0051 0.0478 0.0297 0.0824 0.0167 0.0237 0.0644 0.0269 0.0163 0.0149 0.0239 0.0271 0.0136
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0428 0.2188 0.0044 0.0061 0.0379 0.0231 0.0665 0.0142 0.0189 0.0492 0.0259 0.0119 0.0104 0.0232 0.0272 0.0150
WM-115 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.0395 0.2625 0.0045 0.0060 0.0464 0.0361 0.0800 0.0180 0.0217 0.0470 0.0274 0.0145 0.0176 0.0248 0.0308 0.0167
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0429 0.2674 0.0029 0.0053 0.0359 0.0260 0.0463 0.0137 0.0180 0.0467 0.0217 0.0115 0.0159 0.0240 0.0447 0.0143
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0458 0.2857 0.0046 0.0060 0.0649 0.0310 0.0507 0.0163 0.0230 0.0537 0.0257 0.0167 0.0139 0.0213 0.0346 0.0192
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0457 0.2416 0.0039 0.0058 0.0372 0.0289 0.0700 0.0131 0.0183 0.0453 0.0245 0.0141 0.0140 0.0248 0.0454 0.0203
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0426 0.2646 0.0062 0.0063 0.0492 0.0356 0.0868 0.0163 0.0181 0.0484 0.0265 0.0153 0.0138 0.0258 0.0371 0.0212
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0364 0.2439 0.0037 0.0059 0.0318 0.0232 0.0538 0.0158 0.0192 0.0507 0.0271 0.0217 0.0169 0.0209 0.0497 0.0154
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0378 0.2740 0.0056 0.0062 0.0462 0.0342 0.0642 0.0197 0.0219 0.0568 0.0303 0.0147 0.0168 0.0240 0.0356 0.0174
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.2304 0.0102 0.0130 0.0375 0.0409 0.0572 0.0221 0.0476 0.0372 0.0167 0.0148 0.0306 0.0471 0.0289
WM-115 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.0456 0.2653 0.0134 0.0140 0.0510 0.0804 0.0281 0.0283 0.0556 0.0427 0.0215 0.0179 0.0318 0.0516 0.0298
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0637 0.3205 0.0048 0.0066 0.0531 0.0320 0.0786 0.0199 0.0218 0.0485 0.0250 0.0230 0.0170 0.0326 0.0466 0.0205
WM-115 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.0787 0.3876 0.0055 0.0082 0.0605 0.0401 0.0818 0.0226 0.0265 0.0361 0.0279 0.0213 0.0110 0.0250 0.0358 0.0181