Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5465 1.1765 0.2137 0.3460 0.2463 0.6289 0.4630 0.4255 0.9346 0.2584 0.5682 0.3378 0.2632 0.3759 0.2262 0.3215 0.2392 0.8403 0.2924
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.4951 1.0604 0.1848 0.3802 0.2268 0.6135 0.4115 0.3891 0.8403 0.2463 0.4608 0.2890 0.2193 0.2933 0.2183 0.3279 0.1880 0.7299 0.2618
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5128 1.0753 0.1866 0.3425 0.2525 0.6410 0.4115 0.4184 0.8547 0.2392 0.5155 0.3155 0.2294 0.3115 0.2132 0.3049 0.2070 0.7463 0.2809
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5376 0.9804 0.1898 0.3788 0.2232 0.6410 0.4425 0.4000 0.8696 0.2667 0.4673 0.2976 0.2347 0.3185 0.2326 0.3086 0.1751 0.7634 0.2725
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5650 0.9615 0.1919 0.3195 0.2370 0.6494 0.4425 0.4237 0.9259 0.2611 0.5128 0.3145 0.2577 0.3378 0.2079 0.3145 0.2381 0.7874 0.2793
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5465 1.2547 0.1992 0.3215 0.2381 0.5988 0.4329 0.4049 0.9709 0.2646 0.4695 0.2532 0.2278 0.3788 0.2024 0.3215 0.1862 0.7634 0.2882
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5181 1.2547 0.2020 0.3472 0.2500 0.6061 0.3831 0.9346 0.2506 0.5780 0.2770 0.2331 0.3610 0.2024 0.3448 0.2105 0.7692 0.2660
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5128 1.1876 0.1799 0.3367 0.2232 0.5917 0.4202 0.3534 0.9709 0.2451 0.4951 0.2770 0.2278 0.3559 0.2037 0.3215 0.1799 0.7937 0.3012
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5319 1.1947 0.2053 0.3058 0.2506 0.5882 0.4505 0.3718 0.9804 0.2513 0.5780 0.3068 0.2416 0.3968 0.2062 0.3413 0.2165 0.8265 0.3058
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4739 1.1429 0.1724 0.3401 0.2370 0.6369 0.4167 0.4274 0.9259 0.2463 0.4651 0.2625 0.2299 0.3049 0.2020 0.3268 0.2198 0.6897 0.2681
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5000 1.1919 0.1946 0.3195 0.2695 0.6494 0.4167 0.3677 0.8772 0.2294 0.5714 0.2786 0.2506 0.3030 0.1988 0.3623 0.2342 0.8333 0.2825
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.4975 0.9434 0.1876 0.3413 0.1969 0.5882 0.3891 0.3559 0.8696 0.2237 0.4255 0.2660 0.2899 0.2053 0.2660 0.1629 0.6944 0.2667
RVH-421 0.0000
uM
 24
hr
 2
Odyssey/Microvigene
 0.5348 0.9901 0.1828 0.3145 0.2070 0.5952 0.4237 0.4098 0.9259 0.2304 0.4292 0.2890 0.2404 0.2976 0.1949 0.2994 0.2247 0.7874 0.2967
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5348 1.2642 0.1761 0.3175 0.2273 0.6135 0.4255 0.3559 0.8475 0.2506 0.5076 0.2825 0.2137 0.3690 0.1890 0.3155 0.1996 0.7692 0.2924
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5208 1.1723 0.2114 0.3012 0.2217 0.5650 0.3891 0.9009 0.2469 0.5650 0.2674 0.3125 0.3584 0.1931 0.2710 0.1992 0.7874 0.2976
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5291 1.2092 0.1992 0.3322 0.2037 0.5714 0.4149 0.3623 0.8772 0.2387 0.5465 0.2801 0.1980 0.3831 0.2075 0.2747 0.1873 0.7634 0.3106
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5405 1.1249 0.2146 0.3135 0.2387 0.5780 0.4310 0.4292 0.9434 0.2500 0.5848 0.2916 0.2433 0.3584 0.2137 0.3165 0.2132 0.7813 0.3003
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5051 1.2594 0.1923 0.3125 0.2375 0.6667 0.4310 0.3460 0.8929 0.2584 0.5650 0.2747 0.2203 0.3236 0.2062 0.3311 0.2222 0.7194 0.2882
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5181 1.1820 0.2146 0.3096 0.2410 0.6329 0.4184 0.4546 0.8929 0.2481 0.5952 0.2857 0.3663 0.3534 0.2045 0.3096 0.2232 0.7299 0.2817
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5181 1.0101 0.1898 0.3497 0.2174 0.6250 0.4202 0.3802 0.9524 0.2304 0.4739 0.2841 0.1972 0.3030 0.2174 0.2695 0.1908 0.7407 0.2933
RVH-421 0.0000
uM
 24
hr
 3
Odyssey/Microvigene
 0.5155 1.0101 0.2053 0.3311 0.2326 0.5952 0.4255 0.4587 1.0020 0.2421 0.5025 0.2967 0.2320 0.3322 0.2165 0.3497 0.2370 0.7692 0.2890
RVH-421 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.5714 1.4535 0.2123 0.3205 0.2584 0.6410 0.4425 0.3731 0.2660 0.6289 0.3356 0.2674 0.3953 0.1912 0.3497 0.2165 0.7752 0.3546
RVH-421 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.5435 1.2723 0.2058 0.2941 0.2639 0.5952 0.4739 0.3831 0.9091 0.2611 0.6173 0.3115 0.2433 0.4184 0.1957 0.3425 0.2079 0.7874 0.3226
RVH-421 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.5618 1.1173 0.2079 0.3115 0.2381 0.6024 0.4425 0.3774 0.9804 0.2801 0.5917 0.2874 0.2410 0.3984 0.1996 0.3077 0.1996 0.7874 0.3106
RVH-421 0.0000
uM
 24
hr
 4
Odyssey/Microvigene
 0.6135 1.3736 0.2110 0.2950 0.2591 0.5882 0.4717 0.4098 0.9709 0.2747 0.6329 0.3436 0.2667 0.4016 0.2123 0.3322 0.2342 0.8197 0.3257