Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4608 0.9259 0.1560 0.2525 0.2179 0.7519 0.3937 0.3115 0.8929 0.2232 0.4444 0.2801 0.2101 0.2597 0.1869 0.2985 0.1786 0.2532
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4630 0.9524 0.1754 0.2532 0.2387 0.7634 0.4348 0.3311 0.9091 0.2088 0.5181 0.2770 0.2283 0.2732 0.2000 0.2924 0.2288 0.5587 0.2632
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4831 1.2107 0.1792 0.2710 0.2732 0.7634 0.3861 0.3390 0.7874 0.2342 0.4673 0.2916 0.2119 0.2933 0.1815 0.3571 0.1957 0.5682 0.2717
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4975 1.2005 0.2101 0.2890 0.2639 0.8000 0.3106 0.7937 0.2667 0.6098 0.3049 0.2421 0.2825 0.1908 0.3922 0.2457 0.5747 0.2994
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4505 0.9009 0.1527 0.2817 0.1905 0.6494 0.3425 0.2817 0.8621 0.2024 0.3922 0.2681 0.2416 0.1938 0.2703 0.1745 0.5556 0.2347
RVH-421 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.4831 0.9174 0.1695 0.2849 0.2079 0.6623 0.3745 0.3257 0.8850 0.2101 0.4367 0.2933 0.2160 0.2494 0.2075 0.2967 0.1901 0.2500
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.5051 1.0428 0.1852 0.2695 0.2571 0.8265 0.4032 0.3125 0.9009 0.2375 0.4651 0.2874 0.2262 0.2809 0.1890 0.3115 0.1890 0.5714 0.2762
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4926 0.9709 0.1957 0.2667 0.2494 0.7874 0.4444 0.3012 0.8475 0.2257 0.5181 0.2941 0.2033 0.2618 0.1859 0.2632 0.1984 0.5747 0.2890
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4926 0.8929 0.1541 0.2688 0.2247 0.7042 0.3861 0.2933 0.8772 0.1957 0.4292 0.2994 0.1852 0.2558 0.1908 0.2916 0.1938 0.5405 0.3068
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4608 0.8621 0.1880 0.2571 0.2217 0.6623 0.3861 0.3472 0.8929 0.1916 0.4546 0.2882 0.2179 0.2558 0.1992 0.2833 0.2053 0.5618 0.2597
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4831 1.0917 0.1949 0.2890 0.2513 0.7246 0.3774 0.3086 0.7874 0.2375 0.4739 0.2950 0.2237 0.2481 0.1745 0.3345 0.1848 0.5495 0.2688
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4444 1.0309 0.1866 0.2591 0.2416 0.6993 0.3922 0.3175 0.8000 0.2404 0.4951 0.2762 0.2000 0.2639 0.1988 0.2985 0.2004 0.5848 0.2538
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.5102 0.8696 0.1543 0.3115 0.2020 0.6757 0.3367 0.2793 0.8475 0.1988 0.3846 0.2833 0.1898 0.2232 0.2625 0.1887 0.5181 0.2653
RVH-421 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.4951 0.8850 0.1621 0.3068 0.2033 0.6623 0.3546 0.3623 0.8547 0.2222 0.4348 0.2817 0.2146 0.2427 0.2183 0.2890 0.2079 0.5263 0.2591
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4902 0.9259 0.1689 0.2545 0.2310 0.6803 0.3718 0.2916 0.2137 0.4184 0.2959 0.1883 0.2416 0.2049 0.3257 0.1779 0.5025 0.2732
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4808 0.8333 0.1623 0.2445 0.2519 0.6452 0.3861 0.3030 0.8929 0.1976 0.4444 0.2916 0.2024 0.2558 0.2028 0.2841 0.1848 0.5348 0.2632
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.5465 0.8475 0.1776 0.3040 0.2237 0.6993 0.3817 0.3290 0.9091 0.2488 0.4484 0.3125 0.2278 0.2703 0.3003 0.2079 0.5465 0.2632
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.5848 0.9615 0.1792 0.2833 0.2421 0.6993 0.4098 0.3497 0.9174 0.2551 0.4951 0.2469 0.2710 0.2092 0.2916 0.2033 0.6173 0.2639
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.4951 0.8621 0.1631 0.2924 0.1923 0.6452 0.3226 0.2890 0.9346 0.2198 0.3891 0.2874 0.1949 0.2237 0.2053 0.2463 0.1764 0.4739 0.2825
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.5376 0.8850 0.1600 0.2717 0.1965 0.6369 0.3559 0.2809 0.2770 0.4167 0.2994 0.2079 0.2208 0.2092 0.2667 0.1761 0.4785 0.2717
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
RVH-421 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5587 1.2063 0.1862 0.3690 0.2416 0.6410 0.4525 0.3802 0.9346 0.2681 0.4878 0.2762 0.2416 0.3484 0.2012 0.3345 0.1908 0.7813 0.2762
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5465 1.2453 0.2439 0.4016 0.2740 0.6329 0.3817 0.9091 0.2874 0.5376 0.3030 0.2494 0.3690 0.2604 0.3333 0.2667 0.7813 0.2747
RVH-421 0.0000
uM
 24
hr
 1
Odyssey/Microvigene
 0.5236 1.1364 0.2257 0.3968 0.2294 0.6211 0.4425 0.3937 0.9091 0.2571 0.5051 0.2882 0.2283 0.3460 0.2347 0.3401 0.2119 0.8065 0.2632