Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5780 1.4993 0.2119 0.2710 0.2571 0.6536 0.4348 0.3390 0.3300 0.6410 0.3472 0.2551 0.4149 0.2020 0.4167 0.2033 0.6061 0.3345
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5988 1.3477 0.1901 0.2597 0.2994 0.6329 0.4444 0.3759 0.9709 0.2801 0.6897 0.3390 0.2755 0.4219 0.1783 0.3534 0.2353 0.6061 0.3077
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5525 1.1390 0.1733 0.2710 0.2165 0.5848 0.4098 0.3268 0.9615 0.2703 0.5682 0.3257 0.2342 0.3690 0.2114 0.2762 0.1880 0.5556 0.2681
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.6098 1.3245 0.1942 0.2817 0.2525 0.5747 0.4237 0.3759 0.9804 0.2710 0.5848 0.3390 0.2653 0.4049 0.1818 0.3268 0.2359 0.5848 0.3106
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.4975 1.0846 0.1739 0.2801 0.2212 0.5618 0.3802 0.3145 1.0050 0.2392 0.4831 0.2985 0.2242 0.3268 0.1923 0.2717 0.1672 0.5780 0.2865
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5236 1.0504 0.1764 0.2786 0.2933 0.5525 0.3968 0.3356 0.9091 0.2331 0.5348 0.3077 0.2183 0.3155 0.1859 0.3021 0.2016 0.5848 0.2725
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.4975 0.9434 0.1577 0.2907 0.2188 0.5650 0.3759 0.3279 0.9434 0.2146 0.4032 0.3003 0.2012 0.2725 0.1961 0.2710 0.1812 0.5405 0.2639
RVH-421 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5465 1.1236 0.1786 0.2653 0.2304 0.5714 0.3968 0.3509 0.9615 0.2387 0.4386 0.3322 0.2212 0.3185 0.2020 0.2865 0.2114 0.6024 0.2941
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.5102 1.0741 0.1718 0.2695 0.2268 0.8696 0.3968 0.3115 0.7813 0.2632 0.4878 0.3068 0.2353 0.2703 0.1880 0.3425 0.1988 0.5208 0.2584
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.4673 1.0846 0.2024 0.2725 0.2916 0.8547 0.4310 0.3300 0.7299 0.2445 0.5128 0.3205 0.2404 0.2710 0.1916 0.3534 0.5025 0.2695
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.4405 0.8772 0.1471 0.2825 0.1852 0.6803 0.3521 0.2916 0.7042 0.2247 0.4348 0.2857 0.2049 0.2421 0.2079 0.2770 0.2066 0.4739 0.2193
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.4878 0.8772 0.1773 0.2481 0.2155 0.6993 0.3571 0.3175 0.7143 0.2105 0.4762 0.3058 0.2079 0.2481 0.1931 0.2825 0.1953 0.5102 0.2387
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.4951 1.2788 0.1812 0.2793 0.2755 0.7752 0.4367 0.3257 0.8065 0.2681 0.5525 0.3236 0.2688 0.2941 0.1912 0.4132 0.2299 0.5882 0.2941
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.5102 1.3263 0.2096 0.2755 0.2865 0.8065 0.5128 0.3509 0.7813 0.2703 0.6173 0.2959 0.1976 0.4878 0.5814 0.3040
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.4717 1.0753 0.1805 0.2857 0.2247 0.7299 0.4184 0.3322 0.7752 0.2481 0.4651 0.3185 0.2179 0.2710 0.2045 0.3356 0.1988 0.5263 0.2421
RVH-421 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.5076 1.0604 0.1938 0.2632 0.2439 0.7092 0.4630 0.3509 0.8333 0.2433 0.5128 0.3279 0.2762 0.2890 0.1848 0.3559 0.2283 0.5780 0.2747
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4951 1.1148 0.1802 0.2732 0.2564 0.8547 0.3922 0.3436 0.7813 0.2653 0.4566 0.3185 0.2183 0.2890 0.1721 0.3185 0.2174 0.5525 0.2817
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4926 1.0834 0.1949 0.2660 0.2577 0.8772 0.4348 0.3049 0.8265 0.2469 0.5587 0.2857 0.2008 0.2778 0.1873 0.3155 0.2410 0.5525 0.2725
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4785 0.9804 0.1733 0.2625 0.2359 0.7407 0.3953 0.3021 0.8772 0.2242 0.4739 0.2740 0.2066 0.2941 0.1972 0.2890 0.1675 0.5495 0.2681
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4695 1.0428 0.1880 0.2525 0.2591 0.7937 0.4292 0.3690 0.8772 0.2326 0.5495 0.3058 0.2278 0.2732 0.1812 0.3175 0.2132 0.5714 0.2882
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4739 1.2804 0.1890 0.2825 0.2639 0.7463 0.4292 0.3049 0.7692 0.2410 0.4695 0.3279 0.2119 0.2994 0.1822 0.3610 0.1855 0.5319 0.2674
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4878 1.2887 0.1953 0.2519 0.2525 0.7752 0.4739 0.3584 0.7692 0.2732 0.5952 0.3077 0.2169 0.3236 0.1890 0.3509 0.2252 0.5780 0.2646
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4717 0.9615 0.1667 0.2725 0.2208 0.6944 0.3546 0.2817 0.8197 0.2083 0.4630 0.2703 0.1894 0.2370 0.1855 0.2817 0.1812 0.5102
RVH-421 0.0000
uM
 10
hr
 2
Odyssey/Microvigene
 0.4785 1.0256 0.1748 0.2625 0.2381 0.7042 0.3922 0.3484 0.8547 0.2336 0.4695 0.2924 0.2347 0.2451 0.1873 0.3205 0.2232 0.5556 0.2747
RVH-421 0.0000
uM
 10
hr
 3
Odyssey/Microvigene
 0.5435 1.2048 0.1773 0.2874 0.2558 0.9009 0.4115 0.3215 0.7874 0.2625 0.5128 0.3086 0.2165 0.2924 0.1988 0.3268 0.2083 0.5780 0.2825