Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5650 1.2887 0.2416 0.3788 0.2933 0.6250 0.4587 0.4587 1.0235 0.3175 0.5051 0.3788 0.2604 0.3546 0.2066 0.3021 0.7246 0.3049
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.5495 1.4065 0.2203 0.3236 0.2475 0.6579 0.4425 0.3378 0.9259 0.3077 0.5988 0.3425 0.2500 0.4115 0.1965 0.3650 0.2222 0.6803 0.2976
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.5291 1.3680 0.2232 0.3345 0.6711 0.5236 0.3861 1.0010 0.3165 0.5525 0.3195 0.2732 0.4444 0.2137 0.3300 0.2427 0.6211 0.3012
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.5102 1.1919 0.1835 0.3195 0.2320 0.6289 0.4167 0.3333 0.8772 0.2653 0.5208 0.3106 0.2141 0.3937 0.2079 0.3215 0.1773 0.5618 0.2801
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.5181 1.2821 0.2151 0.3040 0.2457 0.6329 0.4444 0.3788 0.9615 0.2857 0.5988 0.3802 0.2558 0.3610 0.2132 0.3086 0.2123 0.5780 0.2703
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.4975 1.2626 0.2310 0.3195 0.2463 0.6250 0.4049 0.3690 0.9259 0.2786 0.5236 0.3205 0.2315 0.3290 0.2141 0.3448 0.2041 0.6289 0.2611
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.4926 1.2330 0.1988 0.3333 0.2475 0.6452 0.4878 0.3521 0.9524 0.2681 0.5917 0.3509 0.2262 0.3546 0.1953 0.3195 0.2404 0.6024 0.2688
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.4785 1.0893 0.1873 0.3068 0.6024 0.4065 0.3484 0.8621 0.2326 0.4566 0.2874 0.2045 0.3003 0.2128 0.3205 0.1812 0.5495 0.2653
RVH-421 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.4975 1.0893 0.1828 0.2375 0.6024 0.4202 0.3636 0.9174 0.2381 0.5051 0.3115 0.2660 0.3311 0.1898 0.3185 0.2375 0.6211 0.2551
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.5128 1.3123 0.2217 0.3049 0.2252 0.6061 0.4032 0.3215 0.9009 0.2681 0.5102 0.2976 0.2070 0.4049 0.3448 0.1980 0.6579 0.2833
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.5465 1.2270 0.1887 0.3049 0.2564 0.6369 0.3937 0.3247 0.9524 0.2611 0.5780 0.2817 0.2247 0.3953 0.1976 0.3058 0.2058 0.6250 0.2584
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.5128 1.1834 0.1923 0.2967 0.2494 0.6024 0.3861 0.3345 0.9901 0.2564 0.5208 0.2994 0.2475 0.3731 0.2020 0.2801 0.2786 0.5714 0.2833
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.5155 1.2330 0.1996 0.2778 0.6211 0.4310 0.3802 0.9615 0.2359 0.5917 0.2950 0.2740 0.3876 0.1876 0.3390 0.2463 0.6024 0.2625
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.4951 1.3021 0.1822 0.3155 0.2558 0.6711 0.4065 0.3460 0.9346 0.2336 0.5102 0.3012 0.2169 0.3425 0.1984 0.3623 0.1972 0.6024 0.2865
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.4926 1.2438 0.1980 0.3040 0.2681 0.6667 0.4566 0.3677 0.9709 0.3012 0.5952 0.3345 0.2469 0.3448 0.1894 0.3509 0.2045 0.6329 0.2506
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.4785 0.9901 0.2924 0.1946 0.5917 0.3534 0.2907 0.9174 0.2033 0.4348 0.2717 0.1934 0.2907 0.1972 0.2710 0.1678 0.5747 0.2299
RVH-421 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.4785 1.0020 0.1802 0.2959 0.2079 0.5780 0.3704 0.3690 0.9259 0.2342 0.4739 0.2732 0.2137 0.2959 0.1898 0.3175 0.2326 0.6711 0.2611
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5525 1.3514 0.2128 0.3021 0.2740 0.6579 0.4167 0.3690 1.0776 0.2532 0.5917 0.3322 0.2770 0.4016 0.1733 0.2141 0.6410 0.2732
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5405 1.2579 0.1842 0.2882 0.2475 0.6061 0.3876 0.9804 0.2817 0.6494 0.3247 0.2500 0.4065 0.1852 0.3215 0.2128 0.6289 0.3236
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5587 1.2870 0.1919 0.2874 0.2273 0.6410 0.3922 0.3546 1.0173 0.2778 0.5882 0.3290 0.2463 0.4049 0.1976 0.3290 0.1898 0.5882 0.3436
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5319 1.2594 0.2183 0.2604 0.2273 0.5780 0.4115 0.4167 1.0020 0.2740 0.6135 0.3215 0.2667 0.3774 0.1736 0.3413 0.2370 0.6494 0.2874
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5291 1.3755 0.1887 0.2933 0.2114 0.6494 0.4082 0.3623 0.9804 0.2907 0.6024 0.3333 0.3425 0.1789 0.3460 0.2000 0.5848 0.2899
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5155 1.2594 0.2033 0.2688 0.2481 0.5988 0.4032 0.3968 0.9804 0.2577 0.6173 0.2941 0.2364 0.3390 0.1957 0.3226 0.2070 0.6061 0.2874
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5208 1.1173 0.1957 0.2778 0.2398 0.5814 0.3891 0.3401 0.9615 0.2359 0.2985 0.2070 0.3290 0.1927 0.1822 0.5917 0.2985
RVH-421 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.5102 1.1325 0.2119 0.2653 0.2283 0.5650 0.4115 0.4149 1.0331 0.2404 0.5556 0.3236 0.2132 0.3745 0.1905 0.3571 0.2096 0.6061 0.2890