Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5556 0.9434 0.1486 0.3559 0.1866 0.5650 0.3571 0.3448 0.8130 0.2916 0.4525 0.3135 0.2278 0.2916 0.2591 0.2732 0.1678 0.5650 0.3003
RVH-421 0.0000
uM
 5
hr
 1
Odyssey/Microvigene
 0.5848 0.9434 0.1701 0.3534 0.1901 0.5682 0.3677 0.3584 0.8696 0.2809 0.4878 0.3205 0.2545 0.3068 0.2262 0.2725 0.2283 0.6211 0.2532
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5025 1.2837 0.2058 0.2874 0.3484 0.5988 0.3802 0.3448 0.9615 0.2639 0.4975 0.3125 0.2278 0.4274 0.1795 0.3322 0.1862 0.6329 0.2732
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5128 1.2270 0.2132 0.2967 0.3378 0.6098 0.4115 0.3521 0.9901 0.2786 0.5714 0.3003 0.2304 0.3774 0.1876 0.3247 0.2222 0.6494 0.2639
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5155 1.1628 0.1812 0.2976 0.2660 0.5780 0.3831 0.3311 0.9901 0.2433 0.4951 0.2899 0.1984 0.3623 0.1873 0.3290 0.1742 0.5917 0.2710
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.5208 1.2092 0.1976 0.2882 0.2778 0.5952 0.4274 0.9615 0.2370 0.5587 0.3040 0.2506 0.3623 0.1779 0.3247 0.2326 0.6289 0.2890
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.4608 1.1086 0.1701 0.2817 0.2198 0.5181 0.3690 0.3356 0.9901 0.2212 0.4367 0.2755 0.1976 0.3236 0.1792 0.2950 0.1672 0.5495 0.2801
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.4587 1.0989 0.1934 0.2849 0.2242 0.5263 0.4405 0.3472 1.0000 0.2088 0.5000 0.3165 0.2075 0.3390 0.1795 0.3175 0.2364 0.5587 0.2451
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.4651 0.9346 0.1468 0.2994 0.1613 0.4951 0.3333 0.9346 0.2132 0.4000 0.2445 0.1669 0.2688 0.2066 0.2494 0.1698 0.5102 0.2370
RVH-421 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.4651 0.9434 0.1675 0.2762 0.1890 0.5236 0.3497 0.3677 0.9709 0.2137 0.4202 0.2618 0.1988 0.2762 0.2155 0.2849 0.2212 0.5747 0.2778
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5618 1.3369 0.1984 0.2916 0.2577 0.5814 0.4082 0.3584 0.9434 0.2558 0.5952 0.3268 0.2278 0.3861 0.1812 0.3759 0.1873 0.5917 0.2725
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5076 1.2674 0.1957 0.2639 0.2387 0.5814 0.3906 0.3610 0.9346 0.2525 0.6173 0.3236 0.2387 0.3774 0.1919 0.3322 0.2336 0.5988 0.2725
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5319 1.2579 0.2062 0.2755 0.2232 0.5814 0.4167 0.3571 0.9901 0.2604 0.5714 0.3322 0.2208 0.4149 0.1733 0.3401 0.1859 0.6024
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5319 1.2987 0.2299 0.2841 0.2611 0.5465 0.4132 0.4237 0.9174 0.2688 0.6024 0.3058 0.2571 0.4032 0.1866 0.3623 0.2222 0.2950
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.5025 1.2500 0.1946 0.2907 0.2119 0.5747 0.3831 0.3215 0.9346 0.2336 0.5291 0.2849 0.2310 0.3125 0.1953 0.3040 0.1828 0.5682 0.2762
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.4785 1.1186 0.1701 0.2618 0.2128 0.5525 0.3817 0.3817 1.0000 0.2101 0.5405 0.3012 0.2336 0.3106 0.1855 0.2841 0.2008 0.6024 0.2660
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.4695 0.9174 0.1580 0.2976 0.1898 0.5236 0.3484 0.3135 0.9259 0.2058 0.4132 0.2762 0.1887 0.2778 0.2445 0.1709 0.5587 0.2801
RVH-421 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.4878 0.9259 0.1724 0.2933 0.1812 0.5102 0.3584 0.3861 1.0267 0.2037 0.4329 0.2770 0.1980 0.2985 0.2016 0.2571 0.1992 0.5618 0.2695
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5747 1.6000 0.2183 0.2646 0.3125 0.6452 0.4405 0.3425 0.2762 0.6494 0.3534 0.2747 0.4115 0.2016 0.3906 0.2278 0.6024 0.3906
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5917 1.3210 0.2141 0.2916 0.3003 0.5988 0.4695 0.4098 0.9615 0.2994 0.6667 0.3571 0.2577 0.4566 0.1828 0.3968 0.2212 0.6211 0.3322
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5348 1.1249 0.1815 0.2899 0.2169 0.5650 0.4115 0.3759 1.0163 0.2433 0.5319 0.3185 0.2114 0.3906 0.2132 0.3247 0.2273 0.5848 0.2899
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.6135 1.3643 0.1965 0.2967 0.2475 0.5814 0.4219 0.3718 0.9901 0.2793 0.5780 0.3333 0.2500 0.4274 0.1972 0.3497 0.2262 0.5988 0.2924
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.4831 1.0526 0.1761 0.2950 0.2247 0.6098 0.4184 0.3718 1.0549 0.2618 0.5102 0.3058 0.2410 0.3623 0.1912 0.3086 0.5917 0.3802
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5376 1.1050 0.1980 0.3068 0.2506 0.6098 0.4082 0.3623 0.9709 0.2481 0.5747 0.3610 0.2564 0.3279 0.2008 0.3247 0.2288 0.6024 0.2762
RVH-421 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.5000 1.0183 0.2203 0.3745 0.2653 0.6250 0.4255 0.4184 0.9524 0.3106 0.4739 0.3774 0.2320 0.3460 0.2278 0.3279 0.2398 0.6757 0.3003