Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
RVH-421 0.0000
uM
 10
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 10
hr
 8
InnoScan/Mapix
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2066 0.5181 0.0104 0.0073 0.0077 0.0093 0.0146 0.2208 0.0128 0.1890 0.0145 0.0072 0.0050 0.0213 0.0050 0.0173 0.0103 0.0058 0.0825 0.0865
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2381 0.6173 0.0095 0.0025 0.0076 0.0116 0.0152 0.2342 0.0115 0.2604 0.0224 0.0091 0.0055 0.0225 0.0073 0.0207 0.0095 0.0053 0.0897 0.0643
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2237 0.5495 0.0050 0.0043 0.0063 0.0088 0.0110 0.3175 0.0092 0.0076 0.0051 0.0239 0.0051 0.0265 0.0075 0.0073 0.0935 0.0622
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2500 0.6211 0.0069 0.0066 0.0066 0.0108 0.0133 0.2976 0.0090 0.2674 0.0208 0.0087 0.0060 0.0260 0.0077 0.0269 0.0091 0.0062 0.1167 0.0618
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2123 0.4032 0.0081 0.0050 0.0065 0.0094 0.0104 0.3185 0.0098 0.2342 0.0158 0.0072 0.0049 0.0176 0.0050 0.0183 0.0076 0.0059 0.1017 0.1527
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.2463 0.4902 0.0065 0.0058 0.0063 0.0129 0.0124 0.3185 0.0098 0.2532 0.0212 0.0084 0.0059 0.0178 0.0066 0.0289 0.0087 0.0050 0.0903 0.1223
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 24
hr
 1
InnoScan/Mapix
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1410 0.3086 0.0140 0.0110 0.0119 0.0201 0.1698 0.0182 0.2268 0.0150 0.0051 0.0041 0.0147 0.0039 0.0267 0.0141 0.1206 0.0311
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1425 0.3559 0.0110 0.0095 0.0076 0.0152 0.0210 0.1701 0.0166 0.2646 0.0057 0.0043 0.0151 0.0053 0.0276 0.0139 0.0058 0.1171 0.0406
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1894 0.5263 0.0077 0.0065 0.0076 0.0112 0.0164 0.2160 0.0146 0.2703 0.0164 0.0068 0.0050 0.0214 0.0050 0.0185 0.0086 0.0062 0.0884 0.0686
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.2283 0.5587 0.0112 0.0099 0.0079 0.0137 0.0184 0.1938 0.0153 0.2288 0.0177 0.0077 0.0059 0.0233 0.0198 0.0102 0.0054 0.0878 0.0987
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1117 0.4386 0.0080 0.0065 0.0080 0.0130 0.0154 0.1770 0.0158 0.2119 0.0113 0.0073 0.0046 0.0172 0.0033 0.0217 0.0083 0.0054 0.0973 0.0404
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.1295 0.4926 0.0088 0.0082 0.0072 0.0170 0.0166 0.1825 0.0150 0.2273 0.0118 0.0071 0.0058 0.0173 0.0042 0.0193 0.0092 0.0065 0.1014 0.0503
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
RVH-421 0.0000
uM
 24
hr
 2
InnoScan/Mapix
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1387 0.3984 0.0112 0.0087 0.0094 0.0109 0.0185 0.1701 0.0147 0.2525 0.0152 0.0059 0.0041 0.0157 0.0041 0.0392 0.0161 0.1475 0.0391
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1393 0.4630 0.0100 0.0084 0.0072 0.0145 0.0175 0.1905 0.0163 0.0156 0.0059 0.0047 0.0154 0.0061 0.0403 0.0142 0.0061 0.1678 0.0334
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1650 0.5181 0.0057 0.0050 0.0067 0.0090 0.0101 0.2183 0.0105 0.2252 0.0066 0.0041 0.0198 0.0046 0.0192 0.0109 0.0060 0.0851 0.0784
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1647 0.5208 0.0076 0.0072 0.0066 0.0116 0.0129 0.2597 0.0133 0.2326 0.0160 0.0128 0.0058 0.0215 0.0063 0.0209 0.0090 0.0055 0.0890 0.0887
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1449 0.3448 0.0103 0.0107 0.0096 0.0148 0.0196 0.1808 0.0201 0.2252 0.0173 0.0054 0.0039 0.0122 0.0033 0.0295 0.0150 0.0081 0.1164 0.0390
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.1279 0.3663 0.0151 0.0118 0.0090 0.0192 0.0240 0.1795 0.0272 0.2304 0.0144 0.0054 0.0049 0.0119 0.0047 0.0338 0.0152 0.0062 0.1202 0.0286
RVH-421 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.2268 0.4808 0.0053 0.0039 0.0060 0.0124 0.0093 0.2410 0.0071 0.2101 0.0148 0.0065 0.0041 0.0171 0.0073 0.0265 0.0117 0.0067 0.0937 0.0318