Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0041 0.0044 0.0068 0.0050 0.0124 0.0232 0.0820 0.5376 0.1218 0.0038 0.0408 0.0080 0.0515 0.0121 0.0057 0.1425 0.0219
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0539 0.7092 0.0040 0.0044 0.0094 0.0058 0.0097 0.0190 0.0505 0.7143 0.0954 0.0033 0.0164 0.0094 0.0304 0.0050 0.0778 0.0183 0.0102 0.1727 0.0168
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0566 0.7519 0.0037 0.0037 0.0090 0.0068 0.0104 0.0193 0.0523 0.6024 0.1086 0.0033 0.0166 0.0087 0.0047 0.0900 0.0176 0.0103 0.2203 0.0196
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0467 0.4878 0.0042 0.0032 0.0062 0.0047 0.0089 0.0135 0.0605 0.5291 0.1099 0.0026 0.0074 0.0192 0.0065 0.0798 0.0105 0.0069 0.1698 0.0118
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0483 0.3788 0.0045 0.0036 0.0058 0.0053 0.0096 0.0147 0.0655 0.4902 0.1029 0.0028 0.0155 0.0068 0.0263 0.0066 0.0833 0.0115 0.0061 0.1742 0.0121
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0439 0.5780 0.0034 0.0034 0.0078 0.0050 0.0081 0.0156 0.0492 0.8621 0.1018 0.0029 0.0161 0.0090 0.0184 0.0051 0.1057 0.0145 0.0116 0.2392 0.0126
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0453 0.6211 0.0035 0.0033 0.0078 0.0060 0.0089 0.0149 0.0521 0.8333 0.1088 0.0032 0.0162 0.0085 0.0251 0.0053 0.1134 0.0162 0.0099 0.2882 0.0134
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0564 0.5181 0.0035 0.0033 0.0055 0.0043 0.0091 0.0164 0.0678 0.4464 0.1107 0.0029 0.0155 0.0078 0.0217 0.0071 0.0684 0.0087 0.0059 0.1397 0.0154
COLO 858 0.0000
uM
 24
hr
 6
InnoScan/Mapix
 0.0618 0.4926 0.0040 0.0039 0.0049 0.0057 0.0102 0.0205 0.0730 0.4405 0.1068 0.0036 0.0172 0.0098 0.0286 0.0075 0.0697 0.0104 0.0064 0.1546 0.0157
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0556 0.7692 0.0041 0.0036 0.0098 0.0057 0.0096 0.0187 0.0599 0.5952 0.1235 0.0031 0.0176 0.0093 0.0317 0.0051 0.0682 0.0176 0.0097 0.1488 0.0264
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0550 0.7813 0.0037 0.0035 0.0089 0.0102 0.0169 0.0539 0.5405 0.1290 0.0031 0.0176 0.0078 0.0336 0.0050 0.0809 0.0159 0.0103 0.2008 0.0162
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0513 0.4016 0.0042 0.0030 0.0065 0.0043 0.0080 0.0139 0.0633 0.5882 0.1289 0.0027 0.0118 0.0069 0.0196 0.0061 0.0831 0.0099 0.0085 0.1942 0.0137
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0498 0.4348 0.0046 0.0033 0.0065 0.0049 0.0090 0.0137 0.0663 0.5780 0.1235 0.0031 0.0120 0.0070 0.0216 0.0071 0.0896 0.0110 0.0098 0.2222 0.0129
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0529 0.4184 0.0036 0.0035 0.0074 0.0049 0.0101 0.0175 0.0582 0.5780 0.1047 0.0032 0.0130 0.0070 0.0143 0.0050 0.0803 0.0133 0.0084 0.1580 0.0170
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0529 0.4425 0.0040 0.0037 0.0063 0.0052 0.0108 0.0164 0.0585 0.5465 0.1037 0.0034 0.0130 0.0065 0.0152 0.0058 0.0911 0.0137 0.0094 0.1996 0.0135
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0543 0.4673 0.0039 0.0029 0.0057 0.0048 0.0076 0.0123 0.0706 0.4292 0.1198 0.0028 0.0157 0.0079 0.0220 0.0072 0.0694 0.0091 0.0057 0.1223
COLO 858 0.0000
uM
 24
hr
 7
InnoScan/Mapix
 0.0578 0.5025 0.0042 0.0037 0.0059 0.0050 0.0091 0.0149 0.0707 0.4237 0.1277 0.0034 0.0165 0.0088 0.0227 0.0076 0.0532 0.0096 0.0051 0.1577 0.0122
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0501 0.5952 0.0035 0.0030 0.0065 0.0037 0.0075 0.0140 0.0616 0.5525 0.1368 0.0031 0.0143 0.0082 0.0289 0.0074 0.0677 0.0082 0.1351 0.0181
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0527 0.6061 0.0033 0.0028 0.0058 0.0040 0.0079 0.0122 0.0541 0.5291 0.1267 0.0026 0.0148 0.0076 0.0300 0.0053 0.0786 0.0083 0.0087 0.1876 0.0126
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0741 0.5556 0.0037 0.0034 0.0050 0.0039 0.0098 0.0158 0.0880 0.4484 0.1698 0.0034 0.0168 0.0077 0.0281 0.0057 0.0618 0.0085 0.0210 0.1407 0.0201
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0697 0.5291 0.0038 0.0039 0.0060 0.0043 0.0110 0.0182 0.0745 0.4525 0.1558 0.0040 0.0167 0.0078 0.0313 0.0078 0.0675 0.0093 0.0272 0.1572
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0488 0.4329 0.0037 0.0039 0.0054 0.0038 0.0136 0.0672 0.4717 0.1385 0.0026 0.0148 0.0073 0.0169 0.0055 0.0675 0.0097 0.0059 0.1276 0.0153
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0504 0.4149 0.0037 0.0033 0.0057 0.0041 0.0106 0.0149 0.0565 0.4444 0.0029 0.0134 0.0151 0.0055 0.0756 0.0092 0.0065 0.1497 0.0122
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0752 0.5348 0.0044 0.0034 0.0062 0.0043 0.0081 0.0154 0.0824 0.4566 0.1488 0.0029 0.0165 0.0074 0.0206 0.0071 0.0442 0.0090 0.0053 0.1195 0.0142