Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 24
hr
 8
InnoScan/Mapix
 0.0715 0.5263 0.0048 0.0043 0.0061 0.0096 0.0172 0.0722 0.3937 0.1493 0.0036 0.0167 0.0083 0.0218 0.0072 0.0456 0.0094 0.0046 0.1299 0.0166
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.6494 0.0089 0.0078 0.0091 0.0203 0.0554 0.3460 0.0337 0.0032 0.0198 0.0108 0.0331 0.0062 0.0773 0.0147 0.0096 0.0196
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0579 1.0870 0.0054 0.0028 0.0089 0.0092 0.0155 0.0650 0.3484 0.0462 0.0027 0.0210 0.0107 0.0480 0.0075 0.0957 0.0159 0.0104 0.1518 0.0165
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0522 0.4831 0.0041 0.0032 0.0073 0.0099 0.0086 0.0155 0.0602 0.4386 0.0374 0.0028 0.0147 0.0095 0.0263 0.0046 0.1055 0.0118 0.0095 0.1502 0.0253
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0485 0.6289 0.0037 0.0030 0.0087 0.0099 0.0092 0.0136 0.0684 0.4329 0.0423 0.0026 0.0164 0.0107 0.0367 0.0071 0.1166 0.0117 0.0098 0.1799 0.0149
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0581 0.6579 0.0050 0.0032 0.0064 0.0073 0.0081 0.0197 0.0607 0.4292 0.0531 0.0030 0.0186 0.0117 0.0302 0.0047 0.0866 0.0080 0.0106 0.1592 0.0149
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
 0.0566 1.0638 0.0045 0.0027 0.0076 0.0087 0.0086 0.0129 0.0728 0.4425 0.0532 0.0025 0.0212 0.0120 0.0420 0.0064 0.0959 0.0088 0.0111 0.2079 0.0163
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0623 0.5952 0.0056 0.0088 0.0099 0.0100 0.0494 0.3831 0.0351 0.0042 0.0162 0.0246 0.0045 0.0882 0.0135 0.0088 0.1238 0.0277
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0639 0.6135 0.0054 0.0036 0.0098 0.0096 0.0100 0.0188 0.0503 0.3534 0.0344 0.0035 0.0174 0.0097 0.0376 0.0046 0.0995 0.0136 0.0095 0.1650 0.0265
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0458 0.4695 0.0042 0.0035 0.0068 0.0092 0.0089 0.0158 0.0590 0.4167 0.0326 0.0035 0.0133 0.0087 0.0225 0.0075 0.1007 0.0082 0.0097 0.1799 0.0191
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0480 0.4525 0.0037 0.0030 0.0073 0.0108 0.0089 0.0145 0.0619 0.4016 0.0314 0.0028 0.0146 0.0091 0.0311 0.0055 0.1050 0.0104 0.0092 0.2024 0.0169
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0453 0.6135 0.0055 0.0034 0.0056 0.0073 0.0097 0.0165 0.0702 0.5076 0.0525 0.0031 0.0176 0.0100 0.0241 0.0044 0.0934 0.0082 0.0101 0.1927 0.0137
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0497 0.6536 0.0047 0.0027 0.0061 0.0083 0.0085 0.0126 0.0755 0.4808 0.0513 0.0036 0.0195 0.0100 0.0302 0.0048 0.1015 0.0100 0.0084 0.2381 0.0187
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0457 0.3195 0.0037 0.0031 0.0073 0.0102 0.0087 0.0152 0.0556 0.3472 0.0368 0.0028 0.0108 0.0070 0.0163 0.0054 0.0786 0.0089 0.0077 0.1645 0.0164
COLO 858 0.0000
uM
 48
hr
 2
InnoScan/Mapix
 0.0488 0.4237 0.0044 0.0033 0.0071 0.0122 0.0097 0.0144 0.0573 0.3690 0.0397 0.0029 0.0139 0.0084 0.0219 0.0071 0.0806 0.0101 0.0085 0.1799 0.0146
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0570 0.6623 0.0060 0.0116 0.0124 0.0118 0.0259 0.0623 0.3175 0.0433 0.0169 0.0092 0.0287 0.0072 0.0737 0.0190 0.0088 0.1267
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0619 0.6803 0.0046 0.0042 0.0129 0.0142 0.0111 0.0202 0.0633 0.2941 0.0443 0.0038 0.0174 0.0097 0.0298 0.0054 0.0847 0.0186 0.0099 0.1653 0.0250
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0442 0.4098 0.0038 0.0035 0.0064 0.0071 0.0094 0.0160 0.0657 0.3597 0.0317 0.0035 0.0116 0.0080 0.0202 0.0069 0.0894 0.0075 0.0085 0.1504 0.0160
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0415 0.4132 0.0044 0.0028 0.0081 0.0082 0.0086 0.0147 0.0569 0.3509 0.0335 0.0029 0.0124 0.0081 0.0242 0.0061 0.0946 0.0082 0.0079 0.1707 0.0158
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0519 0.7143 0.0047 0.0067 0.0065 0.0109 0.0231 0.0732 0.3984 0.0453 0.0038 0.0183 0.0099 0.0291 0.0044 0.0920 0.0073 0.0148 0.1818 0.0181
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0537 0.7752 0.0054 0.0042 0.0061 0.0071 0.0101 0.0193 0.0697 0.3704 0.0499 0.0039 0.0203 0.0107 0.0299 0.0051 0.1029 0.0082 0.0145 0.2188 0.0155
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0444 0.3484 0.0040 0.0036 0.0074 0.0089 0.0085 0.0154 0.0529 0.2660 0.0304 0.0029 0.0125 0.0065 0.0143 0.0050 0.0672 0.0078 0.0063 0.1332 0.0156
COLO 858 0.0000
uM
 48
hr
 3
InnoScan/Mapix
 0.0452 0.3155 0.0040 0.0036 0.0070 0.0098 0.0090 0.0159 0.0517 0.2710 0.0329 0.0030 0.0111 0.0082 0.0219 0.0063 0.0698 0.0083 0.0068 0.1326 0.0153