Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0480 0.3922 0.0041 0.0035 0.0074 0.0052 0.0097 0.0153 0.0647 0.5882 0.1294 0.0032 0.0188 0.0082 0.0219 0.0062 0.0955 0.0135 0.0064 0.2110 0.0130
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0523 0.4367 0.0037 0.0035 0.0079 0.0052 0.0087 0.0171 0.0562 0.5291 0.1259 0.0030 0.0122 0.0080 0.0192 0.0068 0.0627 0.0150 0.0078 0.1410 0.0168
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0535 0.4673 0.0039 0.0033 0.0082 0.0057 0.0096 0.0162 0.0498 0.4739 0.1203 0.0031 0.0125 0.0073 0.0201 0.0051 0.0729 0.0162 0.0087 0.1842 0.0155
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0544 0.4831 0.0044 0.0028 0.0056 0.0042 0.0099 0.0131 0.0778 0.3984 0.1458 0.0026 0.0134 0.0072 0.0206 0.0084 0.0570 0.0095 0.0055 0.1218 0.0133
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0517 0.3817 0.0043 0.0037 0.0064 0.0046 0.0092 0.0148 0.0804 0.3759 0.1405 0.0036 0.0146 0.0077 0.0257 0.0073 0.0577 0.0109 0.0048 0.1350 0.0121
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0464 0.6024 0.0035 0.0028 0.0066 0.0042 0.0080 0.0140 0.0527 0.6135 0.1133 0.0025 0.0174 0.0082 0.0178 0.0055 0.0907 0.0109 0.0099 0.1773 0.0129
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0461 0.5952 0.0036 0.0033 0.0058 0.0052 0.0089 0.0152 0.0562 0.5747 0.1159 0.0030 0.0194 0.0082 0.0215 0.0061 0.0972 0.0115 0.0103 0.2123 0.0122
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.0623 0.5814 0.0040 0.0029 0.0049 0.0039 0.0069 0.0133 0.0605 0.4831 0.0680 0.0026 0.0187 0.0076 0.0226 0.0070 0.0502 0.0085 0.0059 0.1122 0.0235
COLO 858 0.0000
uM
 24
hr
 3
InnoScan/Mapix
 0.5882 0.0048 0.0038 0.0056 0.0047 0.0091 0.0170 0.0650 0.4926 0.0756 0.0035 0.0195 0.0087 0.0275 0.0085 0.0489 0.0098 0.0058 0.1351 0.0225
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0525 0.6849 0.0043 0.0036 0.0090 0.0053 0.0094 0.0172 0.0512 0.6250 0.1089 0.0030 0.0156 0.0089 0.0294 0.0076 0.0806 0.0198 0.0107 0.1681 0.0313
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0534 0.6849 0.0042 0.0037 0.0096 0.0063 0.0108 0.0172 0.0461 0.5917 0.1111 0.0035 0.0166 0.0084 0.0302 0.0050 0.0188 0.0109 0.0188
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0541 0.4115 0.0040 0.0030 0.0055 0.0039 0.0081 0.0133 0.0786 0.4808 0.1572 0.0030 0.0131 0.0068 0.0213 0.0068 0.0801 0.0089 0.0187 0.1570 0.0222
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0502 0.4310 0.0041 0.0032 0.0066 0.0046 0.0104 0.0164 0.0684 0.4951 0.1393 0.0042 0.0143 0.0071 0.0219 0.0066 0.0769 0.0091 0.0218 0.1764 0.0205
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0494 0.3650 0.0031 0.0061 0.0040 0.0088 0.0145 0.0665 0.5376 0.1475 0.0026 0.0147 0.0067 0.0132 0.0051 0.0721 0.0112 0.0067 0.1445 0.0132
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
 0.0511 0.3636 0.0042 0.0035 0.0064 0.0045 0.0093 0.0144 0.0564 0.4255 0.1191 0.0030 0.0125 0.0062 0.0155 0.0062 0.0767 0.0112 0.0068 0.1855 0.0144
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 4
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0587 0.7874 0.0046 0.0048 0.0107 0.0054 0.0115 0.0234 0.0499 0.5155 0.1241 0.0037 0.0190 0.0103 0.0376 0.0074 0.0722 0.0214 0.0093 0.1368 0.0182
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0547 1.2346 0.0043 0.0052 0.0102 0.0067 0.0127 0.0238 0.0768 0.5291 0.1383 0.0036 0.0200 0.0088 0.0518 0.0074 0.0880 0.0177 0.0133 0.2070 0.0191
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0545 0.5747 0.0044 0.0035 0.0083 0.0041 0.0099 0.0208 0.0738 0.6410 0.1431 0.0028 0.0179 0.0087 0.0303 0.0060 0.0876 0.0102 0.0085 0.1835 0.0118
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0539 0.8547 0.0041 0.0035 0.0064 0.0052 0.0100 0.0207 0.0686 0.6173 0.1534 0.0028 0.0127 0.0103 0.0440 0.0065 0.0963 0.0107 0.0074 0.2217 0.0141
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0582 0.5236 0.0037 0.0049 0.0085 0.0052 0.0091 0.0251 0.0580 0.5988 0.1274 0.0037 0.0138 0.0086 0.0179 0.0053 0.0880 0.0160 0.0096 0.1647 0.0166
COLO 858 0.0000
uM
 24
hr
 5
InnoScan/Mapix
 0.0584 0.7874 0.0047 0.0039 0.0082 0.0061 0.0102 0.0184 0.0549 0.6173 0.1111 0.0033 0.0153 0.0077 0.0280 0.0056 0.1042 0.0138 0.0102 0.2299 0.0176