Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0449 0.3984 0.0037 0.0029 0.0053 0.0046 0.0074 0.0137 0.0715 0.2404 0.1177 0.0025 0.0200 0.0068 0.0125 0.0053 0.0412 0.0079 0.0051 0.0490 0.0155
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0411 0.4425 0.0040 0.0028 0.0045 0.0050 0.0078 0.0120 0.0658 0.2203 0.1133 0.0028 0.0207 0.0071 0.0136 0.0063 0.0427 0.0081 0.0057 0.0615 0.0138
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0578 0.4902 0.0038 0.0027 0.0060 0.0058 0.0075 0.0129 0.0614 0.3846 0.0025 0.0244 0.0073 0.0150 0.0063 0.0732 0.0095 0.0057 0.0144
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0510 0.4098 0.0041 0.0031 0.0054 0.0046 0.0067 0.0158 0.0833 0.3636 0.1439 0.0026 0.0223 0.0092 0.0157 0.0350 0.0088 0.0043 0.0402 0.0223
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0524 0.4000 0.0041 0.0036 0.0052 0.0051 0.0073 0.0141 0.0691 0.3257 0.1314 0.0028 0.0218 0.0074 0.0159 0.0073 0.0395 0.0074 0.0054 0.0554 0.0178
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0781 0.4630 0.0042 0.0036 0.0061 0.0055 0.0103 0.0183 0.0664 0.4546 0.1091 0.0031 0.0269 0.0088 0.0184 0.0070 0.0463 0.0088 0.0045 0.0735 0.0179
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0707 0.4525 0.0045 0.0043 0.0059 0.0060 0.0112 0.0171 0.0707 0.4831 0.1227 0.0267 0.0084 0.0190 0.0074 0.0537 0.0101 0.0055 0.0779 0.0210
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0601 0.5025 0.0046 0.0028 0.0061 0.0055 0.0071 0.0137 0.0876 0.4149 0.1832 0.0027 0.0250 0.0080 0.0157 0.0066 0.0529 0.0096 0.0061 0.0497 0.0230
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
 0.0605 0.4739 0.0047 0.0030 0.0064 0.0060 0.0076 0.0133 0.0748 0.4049 0.1656 0.0029 0.0243 0.0073 0.0133 0.0062 0.0567 0.0100 0.0066 0.0620 0.0204
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 8
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0534 0.5025 0.0042 0.0042 0.0059 0.0040 0.0096 0.0204 0.0694 0.4367 0.1675 0.0034 0.0164 0.0089 0.0298 0.0079 0.0590 0.0099 0.0064 0.1037 0.0168
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0631 0.7752 0.0043 0.0048 0.0069 0.0044 0.0108 0.0200 0.0793 0.4219 0.1887 0.0035 0.0182 0.0086 0.0422 0.0078 0.0675 0.0111 0.0068 0.1414 0.0206
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0692 0.7299 0.0046 0.0033 0.0075 0.0047 0.0096 0.0190 0.0672 0.4926 0.0992 0.0030 0.0209 0.0098 0.0363 0.0075 0.0514 0.0105 0.0053 0.1115 0.0263
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0751 0.8929 0.0049 0.0040 0.0084 0.0055 0.0106 0.0197 0.0765 0.4975 0.1168 0.0033 0.0225 0.0114 0.0481 0.0075 0.0505 0.0130 0.0051 0.1321 0.0316
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0567 0.5917 0.0039 0.0039 0.0058 0.0039 0.0091 0.0205 0.0814 0.4255 0.1585 0.0031 0.0184 0.0100 0.0247 0.0529 0.0094 0.0058 0.1134 0.0147
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0640 0.8696 0.0043 0.0044 0.0063 0.0050 0.0100 0.0208 0.0825 0.4219 0.1534 0.0038 0.0236 0.0099 0.0389 0.0074 0.0561 0.0122 0.0054 0.1387 0.0202
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
COLO 858 0.0000
uM
 24
hr
 1
InnoScan/Mapix
 0.0960 0.6757 0.0053 0.0047 0.0072 0.0061 0.0901 0.5587 0.1161 0.0035 0.0332 0.0609 0.0123 0.0063 0.1466 0.0298
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0475 0.4184 0.0038 0.0036 0.0069 0.0041 0.0073 0.0146 0.0623 0.6757 0.1475 0.0028 0.0119 0.0095 0.0211 0.0055 0.0773 0.0124 0.0097 0.1695 0.0117
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0491 0.4762 0.0037 0.0031 0.0069 0.0049 0.0085 0.0143 0.0740 0.6135 0.1653 0.0029 0.0126 0.0077 0.0295 0.0064 0.0864 0.0123 0.0094 0.2128 0.0134
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0599 0.5076 0.0041 0.0034 0.0060 0.0045 0.0083 0.0159 0.0739 0.5405 0.1350 0.0032 0.0159 0.0251 0.0075 0.0779 0.0093 0.0072 0.1718 0.0162
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0626 0.5747 0.0040 0.0042 0.0062 0.0054 0.0098 0.0818 0.5025 0.1326 0.0038 0.0167 0.0082 0.0078 0.0816 0.0112 0.0068 0.1961 0.0143
COLO 858 0.0000
uM
 24
hr
 2
InnoScan/Mapix
 0.0457 0.3460 0.0044 0.0030 0.0062 0.0041 0.0088 0.0150 0.0695 0.5848 0.1368 0.0027 0.0171 0.0067 0.0136 0.0059 0.0862 0.0103 0.0076 0.1689 0.0114