Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0602 0.3704 0.0040 0.0034 0.0079 0.0062 0.0078 0.0126 0.0706 0.3345 0.1205 0.0036 0.0208 0.0069 0.0145 0.0058 0.0692 0.0104 0.0163 0.0741 0.0330
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0389 0.4739 0.0038 0.0031 0.0083 0.0073 0.0084 0.0141 0.1126 0.5102 0.1996 0.0029 0.0190 0.0062 0.0153 0.0002 0.0946 0.0136 0.0097 0.1112 0.0158
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0402 0.4464 0.0034 0.0035 0.0090 0.0077 0.0092 0.0143 0.0941 0.4951 0.2033 0.0032 0.0199 0.0061 0.0158 0.0034 0.1054 0.0133 0.0106 0.1372 0.0185
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0560 0.5618 0.0043 0.0029 0.0048 0.0052 0.0082 0.0142 0.0671 0.2994 0.0955 0.0029 0.0247 0.0078 0.0178 0.0075 0.0387 0.0060 0.0043 0.0572 0.0143
COLO 858 0.0000
uM
 10
hr
 4
InnoScan/Mapix
 0.0580 0.5465 0.0040 0.0050 0.0064 0.0097 0.0172 0.0709 0.2817 0.1104 0.0037 0.0273 0.0092 0.0200 0.0071 0.0377 0.0085 0.0045 0.0569 0.0142
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0425 0.2558 0.0046 0.0043 0.0112 0.0076 0.0099 0.0188 0.2985 0.0931 0.0033 0.0135 0.0070 0.0118 0.0069 0.0566 0.0240 0.0049 0.0523 0.0167
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0385 0.3759 0.0037 0.0046 0.0103 0.0097 0.0098 0.0184 0.0727 0.2778 0.0981 0.0030 0.0154 0.0063 0.0165 0.0072 0.0560 0.0189 0.0054 0.0642 0.0183
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0410 0.3788 0.0037 0.0032 0.0070 0.0060 0.0076 0.0133 0.0652 0.3333 0.1309 0.0026 0.0186 0.0080 0.0156 0.0090 0.0684 0.0110 0.0056 0.0654 0.0120
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0432 0.4926 0.0042 0.0034 0.0083 0.0074 0.0082 0.0134 0.3155 0.1328 0.0026 0.0214 0.0085 0.0218 0.0060 0.0674 0.0114 0.0049 0.0760 0.0151
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0389 0.3759 0.0054 0.0034 0.0075 0.0064 0.0071 0.0137 0.0651 0.3436 0.1149 0.0032 0.0148 0.0070 0.0126 0.0048 0.1074 0.0128 0.0097 0.1013 0.0119
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0385 0.6329 0.0039 0.0075 0.0078 0.0081 0.0139 0.0724 0.3425 0.1235 0.0035 0.0162 0.0074 0.0184 0.0052 0.1079 0.0108 0.0101 0.1179 0.0145
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0545 0.5917 0.0044 0.0032 0.0061 0.0056 0.0082 0.0163 0.0661 0.3690 0.0829 0.0028 0.0246 0.0087 0.0204 0.0065 0.0429 0.0078 0.0053 0.0510 0.0266
COLO 858 0.0000
uM
 10
hr
 5
InnoScan/Mapix
 0.0603 0.7042 0.0049 0.0039 0.0064 0.0063 0.0090 0.0189 0.0683 0.3584 0.0941 0.0032 0.0261 0.0098 0.0257 0.0072 0.0438 0.0098 0.0049 0.0635 0.0342
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0389 0.2445 0.0031 0.0027 0.0055 0.0046 0.0064 0.0096 0.0599 0.3534 0.0938 0.0022 0.0138 0.0069 0.0116 0.0053 0.0728 0.0087 0.0066 0.0616 0.0117
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0361 0.2740 0.0030 0.0024 0.0052 0.0056 0.0061 0.0092 0.0621 0.3145 0.0985 0.0026 0.0145 0.0061 0.0171 0.0068 0.0842 0.0080 0.0076 0.0745 0.0129
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0600 0.4902 0.0038 0.0028 0.0053 0.0053 0.0070 0.0117 0.0662 0.3636 0.0678 0.0025 0.0216 0.0078 0.0147 0.0072 0.0598 0.0080 0.0055 0.0779 0.0185
COLO 858 0.0000
uM
 10
hr
 6
InnoScan/Mapix
 0.0646 0.4717 0.0040 0.0034 0.0050 0.0065 0.0080 0.0153 0.0649 0.3610 0.0743 0.0031 0.0234 0.0083 0.0214 0.0072 0.0536 0.0090 0.0055 0.0866 0.0185
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0363 0.3546 0.0037 0.0028 0.0063 0.0049 0.0065 0.0119 0.0631 0.3003 0.1346 0.0025 0.0194 0.0090 0.0143 0.0057 0.0507 0.0098 0.0053 0.0507 0.0164
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0393 0.3937 0.0036 0.0026 0.0061 0.0057 0.0067 0.0110 0.0569 0.2674 0.1202 0.0027 0.0195 0.0070 0.0145 0.0052 0.0598 0.0088 0.0059 0.0661 0.0123
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0560 0.3546 0.0043 0.0029 0.0085 0.0065 0.0076 0.0139 0.0580 0.2976 0.1054 0.0029 0.0205 0.0076 0.0149 0.0062 0.0366 0.0099 0.0047 0.0493 0.0244
COLO 858 0.0000
uM
 10
hr
 7
InnoScan/Mapix
 0.0528 0.3788 0.0043 0.0084 0.0080 0.0089 0.0148 0.0587 0.3401 0.1022 0.0032 0.0225 0.0079 0.0066 0.0386 0.0047 0.0551 0.0251