Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | T47D | 316.0 | nM | 6 | hr | 106.87 | 44.20 | p-4EBP1 | 121.81 | |
| MK2206 | T47D | 316.0 | nM | 24 | hr | 130.59 | 62.14 | p-4EBP1 | 310.05 | |
| Triciribine | T47D | 316.0 | nM | 6 | hr | 111.77 | 48.64 | p-4EBP1 | 161.35 | |
| Triciribine | T47D | 316.0 | nM | 24 | hr | 157.83 | 82.22 | p-4EBP1 | 351.16 | |
| Dactolisib | T47D | 316.0 | nM | 6 | hr | 97.57 | 46.09 | p-4EBP1 | 19.39 | |
| Dactolisib | T47D | 316.0 | nM | 24 | hr | 111.62 | 43.30 | p-4EBP1 | 27.62 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 264.89 | 62.49 | p-4EBP1 | 79.32 | |
| Gefitinib | AU565 | 1.0 | uM | 24 | hr | 390.23 | 89.80 | p-4EBP1 | 136.69 | |
| Torkinib | AU565 | 562.0 | nM | 6 | hr | 389.35 | 64.70 | p-4EBP1 | 8.63 | |
| Torkinib | AU565 | 562.0 | nM | 24 | hr | 387.16 | 67.76 | p-4EBP1 | 12.75 | |
| GSK1059615 | AU565 | 1.0 | uM | 6 | hr | 264.43 | 54.13 | p-4EBP1 | 61.31 | |
| GSK1059615 | AU565 | 1.0 | uM | 24 | hr | 423.21 | 75.48 | p-4EBP1 | 91.63 | |
| MK2206 | AU565 | 1.0 | uM | 6 | hr | 371.49 | 74.30 | p-4EBP1 | 132.74 | |
| MK2206 | AU565 | 1.0 | uM | 24 | hr | 346.99 | 77.16 | p-4EBP1 | 117.05 | |
| Triciribine | AU565 | 1.0 | uM | 6 | hr | 316.03 | 71.68 | p-4EBP1 | 93.64 | |
| Triciribine | AU565 | 1.0 | uM | 24 | hr | 471.10 | 88.89 | p-4EBP1 | 126.64 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 296.50 | 62.96 | p-4EBP1 | 8.36 | |
| Dactolisib | AU565 | 1.0 | uM | 24 | hr | 448.62 | 70.82 | p-4EBP1 | 11.25 | |
| Gefitinib | HCC1954 | 1.0 | uM | 6 | hr | 156.16 | 58.86 | p-4EBP1 | 73.75 | |
| Gefitinib | HCC1954 | 1.0 | uM | 24 | hr | 247.74 | 98.10 | p-4EBP1 | 132.98 | |
| Torkinib | HCC1954 | 562.0 | nM | 6 | hr | 246.53 | 53.89 | p-4EBP1 | 11.13 | |
| Torkinib | HCC1954 | 562.0 | nM | 24 | hr | 241.15 | 80.46 | p-4EBP1 | 25.02 | |
| GSK1059615 | HCC1954 | 1.0 | uM | 6 | hr | 273.35 | 59.02 | p-4EBP1 | 37.69 | |
| GSK1059615 | HCC1954 | 1.0 | uM | 24 | hr | 266.55 | 58.39 | p-4EBP1 | 31.57 | |
| MK2206 | HCC1954 | 1.0 | uM | 6 | hr | 275.11 | 71.95 | p-4EBP1 | 104.01 | |