Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | HCC1954 | 1.0 | uM | 24 | hr | 258.18 | 98.30 | p-4EBP1 | 123.38 | |
| Triciribine | HCC1954 | 1.0 | uM | 6 | hr | 268.50 | 82.45 | p-4EBP1 | 129.61 | |
| Triciribine | HCC1954 | 1.0 | uM | 24 | hr | 248.68 | 104.04 | p-4EBP1 | 111.33 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 235.94 | 70.90 | p-4EBP1 | 12.72 | |
| Dactolisib | HCC1954 | 1.0 | uM | 24 | hr | 261.53 | 69.35 | p-4EBP1 | 13.43 | |
| Gefitinib | MCF 10A | 1.0 | uM | 6 | hr | 177.50 | 141.59 | p-4EBP1 | 296.45 | |
| Gefitinib | MCF 10A | 1.0 | uM | 24 | hr | 193.25 | 39.11 | p-4EBP1 | 149.85 | |
| Torkinib | MCF 10A | 562.0 | nM | 6 | hr | 548.27 | 160.67 | p-4EBP1 | 34.82 | |
| Torkinib | MCF 10A | 562.0 | nM | 24 | hr | 466.11 | 102.34 | p-4EBP1 | 39.03 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 6 | hr | 253.19 | 137.65 | p-4EBP1 | 84.97 | |
| GSK1059615 | MCF 10A | 1.0 | uM | 24 | hr | 254.24 | 97.62 | p-4EBP1 | 65.10 | |
| MK2206 | MCF 10A | 1.0 | uM | 6 | hr | 364.05 | 144.66 | p-4EBP1 | 348.04 | |
| MK2206 | MCF 10A | 1.0 | uM | 24 | hr | 333.36 | 178.53 | p-4EBP1 | 206.09 | |
| Triciribine | MCF 10A | 1.0 | uM | 6 | hr | 366.42 | 145.81 | p-4EBP1 | 349.54 | |
| Triciribine | MCF 10A | 1.0 | uM | 24 | hr | 293.94 | 151.27 | p-4EBP1 | 182.99 | |
| Dactolisib | MCF 10A | 1.0 | uM | 6 | hr | 310.00 | 111.77 | p-4EBP1 | 8.07 | |
| Dactolisib | MCF 10A | 1.0 | uM | 24 | hr | 377.56 | 57.43 | p-4EBP1 | 8.40 | |
| Gefitinib | T47D | 1.0 | uM | 6 | hr | 162.18 | 94.35 | p-4EBP1 | 478.48 | |
| Gefitinib | T47D | 1.0 | uM | 24 | hr | 135.21 | 98.09 | p-4EBP1 | 378.64 | |
| Torkinib | T47D | 562.0 | nM | 6 | hr | 147.99 | 68.95 | p-4EBP1 | 37.29 | |
| Torkinib | T47D | 562.0 | nM | 24 | hr | 117.11 | 44.11 | p-4EBP1 | 29.15 | |
| GSK1059615 | T47D | 1.0 | uM | 6 | hr | 161.17 | 62.18 | p-4EBP1 | 336.28 | |
| GSK1059615 | T47D | 1.0 | uM | 24 | hr | 146.44 | 51.05 | p-4EBP1 | 258.70 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 93.95 | 29.78 | p-4EBP1 | 59.88 | |
| MK2206 | T47D | 1.0 | uM | 24 | hr | 130.30 | 53.57 | p-4EBP1 | 296.02 | |