Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | AU565 | 3.16 | nM | 24 | hr | 499.47 | 132.73 | p-4EBP1 | 223.49 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 266.08 | 62.37 | p-4EBP1 | 79.48 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 471.12 | 120.05 | p-4EBP1 | 173.15 | |
| Gefitinib | HCC1954 | 3.16 | nM | 6 | hr | 329.26 | 77.85 | p-4EBP1 | 84.40 | |
| Gefitinib | HCC1954 | 3.16 | nM | 24 | hr | 284.51 | 134.48 | p-4EBP1 | 147.08 | |
| Torkinib | HCC1954 | 0.422 | nM | 6 | hr | 228.09 | 50.66 | p-4EBP1 | 63.64 | |
| Torkinib | HCC1954 | 0.422 | nM | 24 | hr | 277.89 | 115.75 | p-4EBP1 | 127.68 | |
| GSK1059615 | HCC1954 | 3.16 | nM | 6 | hr | 361.17 | 77.71 | p-4EBP1 | 99.96 | |
| GSK1059615 | HCC1954 | 3.16 | nM | 24 | hr | 281.50 | 119.97 | p-4EBP1 | 138.63 | |
| MK2206 | HCC1954 | 3.16 | nM | 6 | hr | 289.40 | 81.72 | p-4EBP1 | 109.94 | |
| MK2206 | HCC1954 | 3.16 | nM | 24 | hr | 297.19 | 127.29 | p-4EBP1 | 144.76 | |
| Triciribine | HCC1954 | 3.16 | nM | 6 | hr | 173.10 | 49.87 | p-4EBP1 | 76.11 | |
| Triciribine | HCC1954 | 3.16 | nM | 24 | hr | 319.70 | 121.37 | p-4EBP1 | 131.18 | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 247.11 | 85.47 | p-4EBP1 | 110.31 | |
| Dactolisib | HCC1954 | 3.16 | nM | 24 | hr | 280.64 | 118.51 | p-4EBP1 | 139.15 | |
| Gefitinib | MCF 10A | 3.16 | nM | 6 | hr | 427.56 | 166.37 | p-4EBP1 | 288.16 | |
| Gefitinib | MCF 10A | 3.16 | nM | 24 | hr | 318.35 | 167.30 | p-4EBP1 | 230.98 | |
| Torkinib | MCF 10A | 0.422 | nM | 6 | hr | 467.62 | 170.70 | p-4EBP1 | 349.85 | |
| Torkinib | MCF 10A | 0.422 | nM | 24 | hr | 359.78 | 160.84 | p-4EBP1 | 247.62 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 6 | hr | 448.80 | 175.75 | p-4EBP1 | 378.05 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 24 | hr | 311.41 | 146.07 | p-4EBP1 | 258.38 | |
| MK2206 | MCF 10A | 3.16 | nM | 6 | hr | 368.90 | 158.40 | p-4EBP1 | 358.95 | |
| MK2206 | MCF 10A | 3.16 | nM | 24 | hr | 294.02 | 162.02 | p-4EBP1 | 256.12 | |
| Triciribine | MCF 10A | 3.16 | nM | 6 | hr | 370.81 | 159.07 | p-4EBP1 | 360.11 | |
| Triciribine | MCF 10A | 3.16 | nM | 24 | hr | 254.77 | 156.64 | p-4EBP1 | 238.19 | |