HMS LINCS Project

Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)

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Small molecules	Cells
10057:MK2206 10098:Gefitinib 10172:GSK1059615 10232:Dactolisib 10280:Triciribine 10345:Torkinib	50091:AU565 50213:HCC1954 50541:T47D 50583:MCF 10A

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Small Molecule	Cell Line	Drug Concentration	Conc Units	Timepoint	Timepoint Units	Measured pERK (Alexa 488)	Measured p-Rb (Alexa 568)	Alexa Fluor 647	Measured p-Akt/p-4EBP1/p-S6 (Alexa 647)	Image
GSK1059615	AU565	3.16	nM	6	hr	200.56	51.17	p-S6	137.70
GSK1059615	AU565	3.16	nM	24	hr	440.35	113.48	p-S6	485.38
MK2206	AU565	3.16	nM	6	hr	319.11	82.93	p-S6	274.09
MK2206	AU565	3.16	nM	24	hr	404.06	121.42	p-S6	486.31
Triciribine	AU565	3.16	nM	6	hr	209.45	53.63	p-S6	117.77
Triciribine	AU565	3.16	nM	24	hr	473.16	122.60	p-S6	505.82
Dactolisib	AU565	3.16	nM	6	hr	233.58	56.94	p-S6	86.02
Dactolisib	AU565	3.16	nM	24	hr	452.52	107.67	p-S6	260.74
Gefitinib	HCC1954	3.16	nM	6	hr	262.69	66.97	p-S6	129.24
Gefitinib	HCC1954	3.16	nM	24	hr	281.78	127.34	p-S6	326.53
Torkinib	HCC1954	0.422	nM	6	hr	202.89	44.42	p-S6	92.64
Torkinib	HCC1954	0.422	nM	24	hr	277.40	106.98	p-S6	408.00
GSK1059615	HCC1954	3.16	nM	6	hr	411.46	92.54	p-S6	333.28
GSK1059615	HCC1954	3.16	nM	24	hr	274.26	111.65	p-S6	305.54
MK2206	HCC1954	3.16	nM	6	hr	319.23	90.52	p-S6	430.33
MK2206	HCC1954	3.16	nM	24	hr	287.75	112.46	p-S6	300.86
Triciribine	HCC1954	3.16	nM	6	hr	165.07	56.66	p-S6	125.32
Triciribine	HCC1954	3.16	nM	24	hr	300.37	110.91	p-S6	306.04
Dactolisib	HCC1954	3.16	nM	6	hr	240.89	89.23	p-S6	235.56
Dactolisib	HCC1954	3.16	nM	24	hr	321.07	124.41	p-S6	414.54
Gefitinib	MCF 10A	3.16	nM	6	hr	407.59	187.11	p-S6	480.67
Gefitinib	MCF 10A	3.16	nM	24	hr	402.15	179.91	p-S6	538.67
Torkinib	MCF 10A	0.422	nM	6	hr	477.98	179.03	p-S6	723.83
Torkinib	MCF 10A	0.422	nM	24	hr	454.80	162.47	p-S6	458.78
GSK1059615	MCF 10A	3.16	nM	6	hr	446.31	184.25	p-S6	782.26

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