Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 1.0 | nM | 24 | hr | 413.46 | 130.22 | p-4EBP1 | 201.44 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 371.79 | 87.94 | p-4EBP1 | 131.82 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 496.90 | 135.91 | p-4EBP1 | 227.34 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 260.00 | 60.48 | p-4EBP1 | 77.46 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 497.45 | 123.10 | p-4EBP1 | 179.16 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 232.57 | 59.57 | p-4EBP1 | 68.44 | |
| Gefitinib | HCC1954 | 1.0 | nM | 24 | hr | 287.16 | 132.24 | p-4EBP1 | 145.96 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 179.96 | 38.18 | p-4EBP1 | 49.70 | |
| Torkinib | HCC1954 | 0.1 | nM | 24 | hr | 294.96 | 117.90 | p-4EBP1 | 132.58 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 397.30 | 83.48 | p-4EBP1 | 105.62 | |
| GSK1059615 | HCC1954 | 1.0 | nM | 24 | hr | 312.64 | 118.83 | p-4EBP1 | 134.00 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 356.01 | 86.76 | p-4EBP1 | 124.05 | |
| MK2206 | HCC1954 | 1.0 | nM | 24 | hr | 296.62 | 131.41 | p-4EBP1 | 160.11 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 163.46 | 53.08 | p-4EBP1 | 72.71 | |
| Triciribine | HCC1954 | 1.0 | nM | 24 | hr | 308.42 | 119.21 | p-4EBP1 | 134.79 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 250.11 | 85.06 | p-4EBP1 | 113.97 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 273.28 | 115.82 | p-4EBP1 | 137.09 | |
| Gefitinib | MCF 10A | 1.0 | nM | 6 | hr | 409.89 | 169.83 | p-4EBP1 | 287.83 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 310.85 | 145.93 | p-4EBP1 | 297.30 | |
| Torkinib | MCF 10A | 0.1 | nM | 6 | hr | 454.27 | 168.14 | p-4EBP1 | 359.55 | |
| Torkinib | MCF 10A | 0.1 | nM | 24 | hr | 351.84 | 149.96 | p-4EBP1 | 312.03 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 6 | hr | 416.58 | 176.11 | p-4EBP1 | 365.78 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 24 | hr | 339.65 | 151.01 | p-4EBP1 | 311.96 | |
| MK2206 | MCF 10A | 1.0 | nM | 6 | hr | 464.75 | 155.56 | p-4EBP1 | 392.89 | |
| MK2206 | MCF 10A | 1.0 | nM | 24 | hr | 310.01 | 157.52 | p-4EBP1 | 304.75 | |