Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | MCF 10A | 0 (control) | 6 | hr | 478.65 | 167.37 | p-4EBP1 | 401.00 | | |
| MK2206 | MCF 10A | 0 (control) | 24 | hr | 294.60 | 141.21 | p-4EBP1 | 347.45 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 480.71 | 167.76 | p-4EBP1 | 402.88 | | |
| Triciribine | MCF 10A | 0 (control) | 24 | hr | 306.71 | 141.21 | p-4EBP1 | 333.89 | | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 444.47 | 164.51 | p-4EBP1 | 404.34 | | |
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 265.37 | 131.31 | p-4EBP1 | 328.31 | | |
| Gefitinib | T47D | 0 (control) | 6 | hr | 193.18 | 93.79 | p-4EBP1 | 445.45 | | |
| Gefitinib | T47D | 0 (control) | 24 | hr | 150.41 | 115.14 | p-4EBP1 | 415.57 | | |
| Torkinib | T47D | 0 (control) | 6 | hr | 176.74 | 87.35 | p-4EBP1 | 505.65 | | |
| Torkinib | T47D | 0 (control) | 24 | hr | 155.50 | 106.52 | p-4EBP1 | 408.57 | | |
| GSK1059615 | T47D | 0 (control) | 6 | hr | 149.75 | 83.94 | p-4EBP1 | 463.86 | | |
| GSK1059615 | T47D | 0 (control) | 24 | hr | 163.50 | 102.73 | p-4EBP1 | 416.56 | | |
| MK2206 | T47D | 0 (control) | 6 | hr | 87.33 | 30.74 | p-4EBP1 | 69.19 | | |
| MK2206 | T47D | 0 (control) | 24 | hr | 156.18 | 103.82 | p-4EBP1 | 379.68 | | |
| Triciribine | T47D | 0 (control) | 6 | hr | 96.15 | 34.17 | p-4EBP1 | 92.30 | | |
| Triciribine | T47D | 0 (control) | 24 | hr | 151.15 | 101.76 | p-4EBP1 | 381.76 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 88.35 | 28.53 | p-4EBP1 | 84.26 | | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 158.04 | 108.23 | p-4EBP1 | 356.79 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 244.78 | 52.81 | p-4EBP1 | 55.56 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 565.14 | 135.83 | p-4EBP1 | 162.84 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 226.90 | 50.34 | p-4EBP1 | 59.83 | |
| Torkinib | AU565 | 0.1 | nM | 24 | hr | 447.23 | 126.56 | p-4EBP1 | 206.68 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 304.69 | 73.43 | p-4EBP1 | 77.12 | |
| GSK1059615 | AU565 | 1.0 | nM | 24 | hr | 447.95 | 122.25 | p-4EBP1 | 188.36 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 323.13 | 77.03 | p-4EBP1 | 111.42 | |